Philip A. Kocheril

Functional Bond-Selective Microscopy: Physically Informed Insights in Chemistry and Biology

Philip A. Kocheril, Ryan E. Leighton, Noor Naji, Dongkwan Lee et autres

Conspectus Biological systems are immensely complex, comprising diverse molecular species organized across multiple spatial and temporal scales. Through intricate networks of tightly regulated chemical reactions, cells are able to maintain homeostasis, respond to environmental stimuli, and meet energetic demands. Despite massive technological …

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Stretching the limits: Decoding hidden biology with vibrational probes

Ziguang Yang, Philip A. Kocheril, Adrian Colazo, Rahuljeet S. Chadha et autres

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MetaboRamics: Highly multiplexed metabolic imaging by stimulated Raman for spatial metabolomics in live cells

Rahuljeet S Chadha, Adrian Colazo, Joseph A Ambarian, Ziguang Yang et autres

Abstract Cellular metabolism is highly dynamic, intertwined and spatially heterogenous, yet methods that can simultaneously visualize multiple metabolic pathways in living systems remain largely limited. Here, we present MetaboRamics: highly multiplexed metabo lic imaging by stimulated Ram an for spatial metabolom ics …

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Toward accurate predictions of bond-selective fluorescence spectra

Philip A. Kocheril, Ryan E. Leighton, Noor Naji, Dongkwan Lee et autres

Vibrational-encoded fluorescence spectro-microscopies are emerging as powerful tools for studying molecular vibrations with the unparalleled sensitivity of fluorescence spectroscopy. We recently described one such technique, termed bond-selective fluorescence-detected infrared-excited (BonFIRE) spectro-microscopy. Currently, prospects of BonFIRE toward rational molecular design are limited, but …

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Optical metabolic imaging of the tricarboxylic acid cycle

Rahuljeet S. Chadha, Philip A. Kocheril, Adrian Colazo, Edmund D. Kapelczak et autres

Abstract The tricarboxylic acid (TCA) cycle lies at the core of cellular metabolism, integrating energy production, biosynthesis and redox homeostasis, yet direct quantitative imaging of its activity in living systems remains challenging. Here we introduce MATRIX-SRS (Metabolic Activity TRacing of the trIcarboXylic …

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Towards accurate predictions of bond-selective fluorescence spectra

Philip A. Kocheril, Ryan Leighton, Noor Naji, Dongkwan Lee et autres

Vibrational-encoded fluorescence spectro-microscopies are emerging as powerful tools for studying molecular vibrations with the unparalleled sensitivity of fluorescence spectroscopy. We recently described one such technique, termed bond-selective fluorescence-detected infrared-excited (BonFIRE) spectro-microscopy. Currently, prospects of BonFIRE towards rational molecular design are limited, but …

Far-field single-molecule vibrational spectroscopy and imaging

Philip A. Kocheril, Haomin Wang, Ryan Eric Leighton, Dongkwan Lee et autres

Single-molecule (SM) optical spectroscopy and imaging approaches have proven transformative, allowing for deep insights into molecular dynamics and behaviour. Despite their intrinsically weaker signals, vibrational spectro-microscopies have advanced significantly within the past decade, allowing for far-field vibrational spectroscopy and imaging at the …

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Single‐Cell Metabolic Imaging Reveals Glycogen‐Driven Adaptations in Endothelial Cells

Rahuljeet Chadha, Benjamin Yang, Dongqiang Yuan, Edmund D. Kapelczak et autres

Endothelial dysfunction (ED) is a defining feature of diabetes mellitus (DM) and a key contributor to many metabolic and cardiovascular diseases. Endothelial cells (ECs) are known to be highly glycolytic and primarily rely on glucose to meet their energy demands. However, the …

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Single-Molecule Vibrational Thermometry

Philip A. Kocheril, Dongkwan Lee, Noor Naji, Rahuljeet Chadha et autres

temperature measurements. Here, we describe Boltzmann-edge vibrational thermometry (BET) detected by anti-Stokes fluorescence, where the relative population of vibrationally excited molecules acts as a calibration-free reporter of local temperature based on the Boltzmann distribution. We demonstrate that BET microscopy is readily compatible …

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Author response for "Two-dimensional bond-selective fluorescence spectroscopy: Violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging"

Philip A. Kocheril, Jiajun Du, Haomin Wang, Ryan Leighton et autres

Two-dimensional bond-selective fluorescence spectroscopy: Violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging

Philip A. Kocheril, Jiajun Du, Haomin Wang, Ryan Leighton et autres

Multidimensional spectroscopies have shaped our understanding of molecular phenomena, but they are often limited in sensitivity. In this work, we describe two-dimensional bond-selective fluorescence-detected infrared-excited (2D-BonFIRE) spectro-microscopy: an ultrasensitive two-dimensional spectroscopy and hyperspectral imaging technique. 2D-BonFIRE spectra are richly detailed, allowing for …

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Single-molecule Vibrational Thermometry

Philip A. Kocheril, Dongkwan Lee, Noor Naji, Rahuljeet Chadha et autres

Molecular probes of temperature (termed “molecular thermometers”) have become broadly used for in situ temperature measurements. Here, we describe Boltzmann-edge vibrational thermometry (BET) detected by anti-Stokes fluorescence, where the relative population of vibrationally excited molecules acts as a calibration-free reporter of local …

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