Alexandra Balola

Computer-guided enzyme engineering of PET hydrolase mutants towards improved PET affinity

Alexandra Balola, Sofia Ferreira, Caio Silva Souza, Diana Lousa et autres

Polyethylene Terephthalate (PET) is an extensively used plastic whose durability and resistance to degradation contribute to growing environmental pollution and concerns. Enzymatic PET degradation, particularly via PETase from Ideonella sakaiensis, has emerged as a sustainable approach due to its ability to depolymerize …

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Additional file 1 of Computer-guided enzyme engineering of PET hydrolase mutants towards improved PET affinity

Alexandra Balola, Sofia Ferreira, Caio Silva Souza, Diana Lousa et autres

Supplementary Material 1

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Additional file 1 of Computer-guided enzyme engineering of PET hydrolase mutants towards improved PET affinity

Alexandra Balola, Sofia Ferreira, Caio Silva Souza, Diana Lousa et autres

Supplementary Material 1

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Computer-guided enzyme engineering of PET hydrolase mutants towards improved PET affinity

Alexandra Balola, Sofia Ferreira, Caio Silva Souza, Diana Lousa et autres

Abstract Polyethylene Terephthalate (PET) is an extensively used plastic whose durability and resistance to degradation contribute to growing environmental pollution and concerns. Enzymatic PET degradation, particularly via PETase from Ideonella sakaiensis, has emerged as a sustainable approach due to its ability to …

From plastic waste to bioprocesses: Using ethylene glycol from polyethylene terephthalate biodegradation to fuel Escherichia coli metabolism and produce value-added compounds

Alexandra Balola, Sofia Ferreira, Isabel Rocha

metabolic and protein engineering strategies. Consequently, we assess the potential of EG as a versatile alternative to conventional carbon sources like glucose, facilitating the closure of the loop between the highly available PET waste and the production of valuable biochemicals. This review …

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Computer-aided design and implementation of efficient biosynthetic pathways to produce high added-value products derived from tyrosine in Escherichia coli

Sofia Ferreira, Alexandra Balola, Anastasia Sveshnikova, Vassily Hatzimanikatis et autres

Developing efficient bioprocesses requires selecting the best biosynthetic pathways, which can be challenging and time-consuming due to the vast amount of data available in databases and literature. The extension of the shikimate pathway for the biosynthesis of commercially attractive molecules often involves …

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Molecular determinants of the SARS-CoV-2 fusion peptide activity

Carolina C. Buga, Mariana Valério, Alexandra Balola, Ana Sofia Ferreira et autres

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, emerged in late 2019 and quickly spread worldwide, resulting in over 125 million infections and 2.7 million deaths as of March 2021 accordingly to the World Health Organization. Despite the great advances achieved by …

Adaptation and Survival of Burkholderia cepacia and B. contaminans During Long-Term Incubation in Saline Solutions Containing Benzalkonium Chloride

Mariana Tavares, Mariya Kozak, Alexandra Balola, Carla P. Coutinho et autres

The Burkholderia cepacia complex (Bcc) is a group of opportunistic pathogenic bacteria with a remarkable metabolic capacity and broad genotypic/phenotypic plasticity, allowing their adaptation to hostile conditions, including nutrient depleted solutions containing antimicrobial agents. Bcc bacteria are feared contaminants in pharmaceutical industries …

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Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products

Mariana Tavares, Mariya Kozak, Alexandra Balola, Isabel Sá‐Correia

complex (Bcc), comprising 24 closely related opportunistic pathogenic species. These bacteria have a widespread environmental distribution, an extraordinary metabolic versatility, a complex genome with three chromosomes, and a high capacity for rapid mutation and adaptation. Additionally, they present an inherent resistance to …

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