Yoshiki Hashimoto

Establishment of monkey jejunum CRYPT-derived cells to investigate species difference between monkeys and humans

Hiroyuki Kusuhara, Ikuko Kaisaki, Yoshiki Hashimoto, Kenpei Tai et autres

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Quantitative analysis of the impact of C421A mutation on BCRP activity in human ileal CRYPT-derived differentiated cells

Mana Namai, Yoshiki Hashimoto, Kazuyoshi Michiba, Osamu Shimomura et autres

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Investigation of differentiation conditions for canine duodenum, upper jejunum, and ileum crypt-derived cells for drug transport and metabolism

Hiroki Takezaki, Yoshiki Hashimoto, Ikuko Kaisaki, Masaki Kinehara et autres

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Characterization of intestinal transporters in human ileal spheroid–derived differentiated cells for the prediction of intestinal drug absorption

Kazuyoshi Michiba, Mana Namai, Yoshiki Hashimoto, Osamu Shimomura et autres

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Three- and four-stranded nucleic acid structures and their ligands

Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi et autres

Nucleic acids have the potential to form not only duplexes, but also various non-canonical secondary structures in living cells. Non-canonical structures play regulatory functions mainly in the central dogma. Therefore, nucleic acid targeting molecules are potential novel therapeutic drugs that can target …

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Development of the serotonin release assay with enterochromaffin cell-rich human intestinal organoids for the risk evaluation of drug-induced emesis

Yoshiki Hashimoto, Kazuya Maeda, Osamu Shimomura, Yoshihiro Miyazaki et autres

Drug-induced emesis is one of the major symptoms of gastrointestinal toxicity. Preclinical risk assessment of emesis has been challenging owing to the lack of suitable animal models or in vitro assay systems. One of the triggers of emesis is an excessive serotonin …

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Development of serotonin release assay with cultured enterochromaffin cells for the risk assessment of emesis induced by tyrosine kinase inhibitors

Yoshiki Hashimoto, Kazuya Maeda, Osamu Shimomura, Yoshihiro Miyazaki et autres

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Importance of Drug Transporters as Determinants of Pharmacokinetics of Drugs

Tomoki Koishikawa, Yoshiki Hashimoto, Hiroyuki Kusuhara

薬物トランスポーターは、多様な薬物を基質として認識し、生体内において血中滞留性や組織分布の決定要因として重要な役割を果たす。本稿では、筆者らの研究成果も含めて、薬物トランスポーター研究に関して、分子論から臨床研究までを紹介する。①薬物トランスポーターの立体構造が解かれ、多様な化合物を基質として認識する機構や輸送駆動力に関する知見が得られた。②microphysiological system/complex in vitro systemの登場により、ヒト臓器機能を模倣したin vitroモデルの開発が進展した。③薬物トランスポーターの輸送活性を反映したバイオマーカーを利用することで、薬物相互作用研究など、薬物トランスポーターの輸送活性の変動を評価することが可能となった。

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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition

Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi, Daisuke Miyoshi

Sequence-selective G-quadruplex ligands are valuable for controlling gene expression. Here, we established a new fluorescence displacement assay using a NRAS G-quadruplex selective fluorescent probe to identify sequence-selective DNA G-quadruplex ligands. These sequence-selective NRAS G-quadruplex ligands retained their binding affinity even in the …

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Evaluation of the risk of diarrhea induced by epidermal growth factor receptor tyrosine kinase inhibitors with cultured intestinal stem cells originated from crypts in humans and monkeys

Yoshiki Hashimoto, Kazuya Maeda, Osamu Shimomura, Yoshihiro Miyazaki et autres

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Study of 18.2-42.0GHz injection-locked frequency doubler with transformer input

Yoshiki Hashimoto, Naoki Kaneda, Kiyotaka Komoku, Nobuyuki Itoh

This paper presents ultra-wideband 18.2-42.0GHz LC injection-locked frequency doubler (ILFD) with transformer inputs. To expand the locking range, the proposed ILFD incorporates the transformer connected to the source of the gain-cell transistor to obtain high linearity of the input circuitry, moreover, the …

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Simple and fast screening for structure-selective G-quadruplex ligands

Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda et autres

Structural selectivity of G-quadruplex ligands is essential for cellular applications since there is an excess of nucleic acids forming duplex structures compared to G-quadruplex structures in living cells. In this study, we developed new structure-selective G-quadruplex ligands utilizing a simple and fast …

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