Swapna Nandakumar

Single Cell Transcriptomics and Surface Protein Expression Reveal Distinct Cellular and Molecular Phenotypes in Human RPESC-RPE and PSC-RPE

Swapna Nandakumar, Farhad Farjood, Taylor Bertucci, Steven Lotz et autres

Current retinal pigment epithelium (RPE) cell replacement strategies in trials for age-related macular degeneration (AMD) are based on either pluripotent stem cell (PSC) or adult RPE stem cell (RPESC) sources. We used Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Seq) to …

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Single-cell transcriptome and surfaceome profiling of the adult human retinal pigment epithelium

Farhad Farjood, Swapna Nandakumar, Taylor Bertucci, Thomas R. Kiehl et autres

The retinal pigment epithelium (RPE) is a pigmented monolayer of cells beneath the neural retina that supports photoreceptor cell function essential for vision. Our study explores the diversity of adult human RPE subpopulations and associated implications for retinal biology. Employing cellular indexing …

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Corrigendum to “Derivation of three induced pluripotent stem cell lines under feeder-free culture conditions from peripheral blood mononuclear cells (PBMC) of Indian patients suffering from inherited retinal diseases carrying different mutations” [Stem Cell Res. 45 (2020) 101757]

Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Rajani Battu, Rajarshi Pal

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Neuronal and cardiac toxicity of pharmacological compounds identified through transcriptomic analysis of human pluripotent stem cell-derived embryoid bodies

Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Harshini Surendran, Savita Datar et autres

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Interventional Strategies for Parkinson Disease: Can Neural Precursor Cells Forge a Path Ahead?

Swapna Nandakumar, Pradnya Shahani, Indrani Datta, Rajarshi Pal

Neural precursor cells (NPCs), derived from pluripotent stem cells (PSCs), with their unique ability to generate multiple neuronal and glial cell types are extremely useful for understanding biological mechanisms in normal and diseased states. However, generation of specific neuronal subtypes (mature) from …

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Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration

Harshini Surendran, Swapna Nandakumar, Vijay Bhaskar Reddy K, Jonathan Stoddard et autres

BACKGROUND: Age-related macular degeneration (AMD) is a result of degeneration/damage of the retinal pigment epithelium (RPE) while retinitis pigmentosa (RP), an inherited early-onset disease, results from premature loss of photoreceptors. A promising therapeutic approach for both is the replacement of lost/damaged cells …

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Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors

Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Harshini Surendran, Rajarshi Pal

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Human Induced Pluripotent Stem Cell-Derived Lung Epithelial System for SARS-CoV-2 Infection Modeling and Its Potential in Drug Repurposing

Harshini Surendran, Swapna Nandakumar, Rajarshi Pal

The lung is the most vulnerable target for the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, and respiratory failure causing acute respiratory distress syndrome is its foremost outcome. However, the current primary in vitro models in use for SARS-CoV-2 display apparent …

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Transplantation of retinal pigment epithelium (RPE) and photoreceptor progenitors (PRP) generated through a xeno-free unified protocol showed improved visual sensitivity in two rodent models

Rajarshi Pal, Harshini Surendran, Swapna Nandakumar, Vijay Bhaskar Reddy Konala et autres

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Derivation of three induced pluripotent stem cell lines under feeder-free culture conditions from peripheral blood mononuclear cells (PBMC) of Indian patients suffering from inherited retinal diseases carrying different mutations

Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Rajani Battu

Inherited retinal diseases (IRDs) are clinically and genetically heterogenous diseases affecting the neural retina and retinal pigment epithelium resulting in irreversible blindness. Owing to advantages like ease of access for treatment, one eye being a perfect natural control for the other, and …

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Spermine protects alpha-synuclein expressing dopaminergic neurons from manganese-induced degeneration

Bejoy Vijayan, Vishnu Raj, Swapna Nandakumar, Asha Kishore et autres

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Autophagy enhancement is rendered ineffective in presence of α-synuclein in melanoma cells

Swapna Nandakumar, Bejoy Vijayan, Asha Kishore, Anoopkumar Thekkuveettil

Increased cellular concentration of α-synuclein (α-syn) predisposes it to misfolding and aggregation that in turn impair the degradation pathways. This poses a limitation to the use of overexpression models for studies on α-syn clearance by autophagy, which is widely investigated for its …

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