Kacie L. Rich

Mammals that can develop type 2 diabetes have a similarly structured β-sheet amyloid oligomer

Brynn E. LeMasters, Caitlyn R. Fields, Justin P. Lomont, Farzaneh Chalyavi et autres

S. Using two-dimensional infrared spectroscopy, we monitor the structure of IAPP from seven different mammals, five of which are from species that can develop type 2 diabetes (ferret, raccoon, cat, baboon, and human) and three from those that do not (hamster, rat, …

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A structural model of toxic amyloid oligomers involved in type 2 diabetes

Shivani T. Shivani, Brynn E. LeMasters, Thirupathi Ravula, Harrison J. Esterly et autres

Amyloid oligomers of the human islet amyloid polypeptide (hIAPP) are a likely cytotoxic species driving β-cell death in type 2 diabetes, but their transient nature has precluded atomic-level structural characterization. We obtained a high-resolution structure of a physiologically relevant hIAPP oligomer. Using …

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Monolayer Sensitivity Enables a 2D IR Spectroscopic Immuno-biosensor for Studying Protein Structures: Application to Amyloid Polymorphs

Joshua S. Ostrander, Justin P. Lomont, Kacie L. Rich, Vivek Saraswat et autres

Immunosensors use antibodies to detect and quantify biomarkers of disease, though the sensors often lack structural information. We create a surface-sensitive two-dimensional infrared (2D IR) spectroscopic immunosensor for studying protein structures. We tether antibodies to a plasmonic surface, flow over a solution …

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Enhancing the signal strength of surface sensitive 2D IR spectroscopy

Megan K. Petti, Joshua S. Ostrander, Vivek Saraswat, Erin R. Birdsall et autres

Spectroscopic techniques that are capable of measuring surfaces and interfaces must overcome two technical challenges: one, the low coverage of molecules at the surface, and two, discerning between signals from the bulk and surface. We present surface enhanced attenuated reflection 2D infrared …

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Structural Insights to Toxic Amylin Oligomers from 2D IR Spectroscopy

Kacie L. Rich, Megan K. Petti, Martin T. Zanni

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Spectroscopic Signature for Stable β-Amyloid Fibrils versus β-Sheet-Rich Oligomers

Justin P. Lomont, Kacie L. Rich, Michał Maj, Jia-Jung Ho et autres

We use two-dimensional IR (2D IR) spectroscopy to explore fibril formation for the two predominant isoforms of the β-amyloid (Aβ 1-40 and Aβ 1-42 ) protein associated with Alzheimer’s disease. Two-dimensional IR spectra resolve a transition at 1610 cm –1 in Aβ …

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Site-specific detection of protein secondary structure using 2D IR dihedral indexing: a proposed assembly mechanism of oligomeric hIAPP

Michał Maj, Justin P. Lomont, Kacie L. Rich, Ariel M. Alperstein et autres

Human islet amyloid polypeptide (hIAPP) aggregates into fibrils through oligomers that have been postulated to contain α-helices as well as β-sheets.

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CCVJ Is Not a Simple Rotor Probe

Christopher A. Rumble, Kacie L. Rich, Gang He, Mark Maroncelli

The photochemistry of the rotor probe 9-(2-carboxy-2-cyanovinyl)julolidine (CCVJ) was studied to elucidate a curious effect of fluid flow previously reported. The apparent sensitivity to fluid motion observed in CCVJ but not in the closely related molecule 9-(dicyanovinyl)julolidine (DCVJ) is found to be …

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THE PHOTOPHYSICAL PROBE, 7‐AZATRYPTOPHAN, IN SYNTHETIC PEPTIDES

Kacie L. Rich, Michel Négrerie, Jianyu Li, Sarah F. Elliott et autres

Abstract 7‐Azatryptophan is proposed as an alternative to tryptophan as a photophysical probe in the study of protein structure and dynamics. Not only are the spectral characteristics of 7‐azatryptophan easily distinguishable from those of tryptophan, but this nonnatural amino acid is shown …

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