Accès ouvert
2026
article
OpenAlex
Mai Fujiwara, Mitsumasa Abe, Yota Horioka, Yoshifumi Fukunishi et autres
The enzymatic reaction mechanism of the serine protease neuropsin (KLK8) was investigated using QM/MM calculations combined with MD simulations. The out-of-plane distortion of the scissile amide bond in the substrate-binding complex contributed to a reduction in the activation barrier. Nucleophilic attack by …
jp
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Accès ouvert
2025
article
OpenAlex
Gopal Murugaiyan, Johnna Francis Varghese, Mai Fujiwara, Lucien P. Garo et autres
Abstract Description IFN-β, a type I interferon, has been used as a first-line therapy for patients with multiple sclerosis (MS) for thirty years; however, the cellular and molecular basis of its therapeutic efficacy remains largely unknown. Here, we found that IFN-β and …
us
(code pays fourni par la source)
2025
article
OpenAlex
Johnna Francis Varghese, Mai Fujiwara, Amrendra K. Ajay, Lucien P. Garo et autres
IFN-β, a type I interferon, has been used as a first-line therapy for patients with multiple sclerosis (MS) for more than 30 years; however, the cellular and molecular basis of its therapeutic efficacy remains unclear. Here, we first used experimental autoimmune encephalomyelitis …
us, ca
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Masami Lintuluoto, Yota Horioka, Mai Fujiwara, Mitsumasa Abe et autres
Serine protease neuropsin is highly expressed in the central nervous system, which regulates brain cognitive function through its proteolytic activity. Neuropsin cleaves heparan sulfate proteoglycan bound to neuregulin-1 (NRG-1) at three specific sites, liberating the ligand domain of NRG-1, which, in turn, …
jp
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Accès ouvert
2023
article
OpenAlex
Mai Fujiwara, Lucien P. Garo, Amrendra K. Ajay, Alkeiver S Cannon et autres
Transforming growth factor β (TGF-β) is critical to the maintenance of intestinal immune homeostasis. Here, we present techniques for analyzing Smad molecules downstream of TGF-β receptor signaling in dextran-sulfate-sodium-induced colitic mice. We describe colitis induction, cell isolation, and flow cytometric cell sorting …
us
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2022
article
OpenAlex
Mai Fujiwara, Radhika Raheja, Lucien P. Garo, Amrendra K. Ajay et autres
A disequilibrium between immunosuppressive Tregs and inflammatory IL-17–producing Th17 cells is a hallmark of autoimmune diseases, including multiple sclerosis (MS). However, the molecular mechanisms underlying the Treg and Th17 imbalance in CNS autoimmunity remain largely unclear. Identifying the factors that drive this …
us
(code pays fourni par la source)
2022
conference-abstract
OpenAlex
Murugaiyan Gopal, Mai Fujiwara, Radhika Raheja, Lucien P. Garo et autres
Abstract A disequilibrium between immunosuppressive regulatory T cells (Tregs) and inflammatory interleukin (IL)-17-producing Th17 cells is a hallmark of autoimmune diseases, including multiple sclerosis (MS). However, molecular mechanisms underlying Treg and Th17 imbalance in the central nervous system (CNS) autoimmunity remain largely …
us
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Accès ouvert
2022
article
OpenAlex
Amrendra K. Ajay, Li Zhao, Shruti Vig, Mai Fujiwara et autres
Signal transduction and activator of transcription 3 (STAT3) is a key transcription factor implicated in the pathogenesis of kidney fibrosis. Although Stat3 deletion in tubular epithelial cells is known to protect mice from fibrosis, vFoxd1 cells remains unclear. Using Foxd1-mediated Stat3 knockout …
us, cn
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Accès ouvert
2022
article
OpenAlex
Chaitali P. Basole, Rebecca K. Nguyen, Katie Lamothe, Puja Billis et autres
After decades of development, inhibitors targeting cyclic nucleotide phosphodiesterases (PDEs) expressed in leukocytes have entered clinical practice for the treatment of inflammatory disorders, with three PDE4 inhibitors being in clinical use as therapeutics for psoriasis, psoriatic arthritis, chronic obstructive pulmonary disease and …
us
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Accès ouvert
2021
article
OpenAlex
Galina Gabriely, Duanduan Ma, Shafiuddin Siddiqui, Linqing Sun et autres
MCs promote tumor growth and offer therapeutic intervention to target these cells in cancer.
us
(code pays fourni par la source)
2021
conference-abstract
OpenAlex
Murugaiyan Gopal, Lucien P. Garo, Amrendra K. Ajay, Mai Fujiwara et autres
Abstract Interleukin-17 (IL-17) is a major inflammatory cytokine implicated in colorectal cancer (CRC) development. However, the mechanisms that control tumorigenic IL-17 signaling are poorly understood. Recently, expression changes and polymorphisms in the small non-coding RNA, microRNA-146a (miR-146a), have been associated with clinical …
us
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Accès ouvert
2021
article
OpenAlex
Lucien P. Garo, Amrendra K. Ajay, Mai Fujiwara, Galina Gabriely et autres
Chronic inflammation can drive tumor development. Here, we have identified microRNA-146a (miR-146a) as a major negative regulator of colonic inflammation and associated tumorigenesis by modulating IL-17 responses. MiR-146a-deficient mice are susceptible to both colitis-associated and sporadic colorectal cancer (CRC), presenting with enhanced …
us
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