Masako Iwai

Identification and functional analysis of class 3 lipases associated with triacylglycerol metabolism in Nannochloropsis oceanica (Eustigmatophyceae)

Masako Iwai, Koichi Hori, Tomokazu Kurita, Kumiko Okazaki et autres

Microalgae accumulate triacylglycerols (TAGs) as storage lipids in response to nutrient limitation, yet the mechanisms governing TAG turnover remain incompletely understood. In the oleaginous microalga Nannochloropsis oceanica, functional studies of TAG degradation have focused on lipases containing patatin domains, whereas the roles …

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Knockout of an SPX -related gene for polyphosphate synthetase accelerates phosphate starvation responses in the oleaginous microalga Nannochloropsis oceanica

Kumiko Okazaki, Koichi Hori, Masako Iwai, Tomokazu Kurita et autres

SPX domain-containing proteins are important for phosphate (Pi) signaling and homeostasis in various eukaryotes. Genomic and transcriptomic analyses of the oleaginous microalga Nannochloropsis oceanica identified four genes encoding SPX family members with distinct domain architectures: SPX (NoSPX1), SPX-VTC (NoSPX2), SPX-EXS (NoSPX3), and …

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Phosphatidic acid phosphohydrolase modulates glycerolipid synthesis in Marchantia polymorpha and is crucial for growth under both nutrient-replete and -deficient conditions

Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara et autres

MAIN CONCLUSION: The phosphatidic acid phosphohydrolase of Marchantia polymorpha modulates plastid glycolipid synthesis through the ER pathway and is essential for normal plant development regardless of nutrient availability. Membrane lipid remodeling is one of the strategies plant cells use to secure inorganic …

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Phosphatidic acid phosphohydrolase modulates glycerolipid synthesis in Marchantia polymorpha and is crucial for growth under both nutrient-replete and -deficient conditions

彩 下城, Misao Shimojo, 将 中村, Masashi Nakamura et autres

Genome editing for biodiesel production in oleaginous microalga, Nannochloropsis species

Tomokazu Kurita, Masako Iwai, Hiroyuki Ohta, Tetsushi Sakuma et autres

Algae are oxygen-producing photosynthetic aquatic organisms. Algal biodiesels have attracted attention because these fuels are produced via photosynthesis, which assimilates CO2. Algal biodiesels are sustainable, not competitive with food production, and have higher productivity compared with terrestrial plants. However, the production costs …

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Crystal structures of photosystem II from a cyanobacterium expressing psbA in comparison to psbA reveal differences in the D1 subunit

Yoshiki Nakajima, Natsumi Ugai-Amo, Naoki Tone, Akiko Nakagawa et autres

Three psbA genes ( psbA 1 , psbA 2 , and psbA 3 ) encoding the D1 subunit of photosystem II (PSII) are present in the thermophilic cyanobacterium Thermosynechococcus elongatus and are expressed differently in response to changes in the growth environment. …

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Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga

Tomokazu Kurita, Masako Iwai, Keishi Moroi, Kumiko Okazaki et autres

Algal lipids are expected to become a basis for sustainable fuels because of the highly efficient lipid production by photosynthesis accompanied by carbon dioxide assimilation. Molecular breeding of microalgae has been studied to improve algal lipid production, but the resultant gene-modified algae …

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Recycling of the major thylakoid lipid MGDG and its role in lipid homeostasis in Chlamydomonas reinhardtii

Masako Iwai, Yui Yamada‐Oshima, Kota Asami, Takashi Kanamori et autres

Monogalactosyldiacylglycerol (MGDG), the most abundant lipid in thylakoid membranes, is involved in photosynthesis and chloroplast development. MGDG lipase has an important role in lipid remodeling in Chlamydomonas reinhardtii. However, the process related to turnover of the lysogalactolipid that results from MGDG degradation, …

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Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes

Masako Iwai, Shiori Shibata, Hiroyuki Ohta, Koichiro Awai

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Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES‐2145

Tomokazu Kurita, Keishi Moroi, Masako Iwai, Kumiko Okazaki et autres

Algae accumulate large amounts of lipids produced by photosynthesis, and these lipids are expected to be utilized as feedstocks for sustainable new energies, known as biodiesels. Nannochloropsis species are eukaryotic microalgae that produce high levels of lipids. However, since the production costs …

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MYB‐like transcription factor NoPSR1 is crucial for membrane lipid remodeling under phosphate starvation in the oleaginous microalga Nannochloropsis oceanica

Hiroki Murakami, Natsue Kakutani, Yunato Kuroyanagi, Masako Iwai et autres

Membrane lipid remodeling under phosphate (Pi) limitation, a process that replaces structural membrane phospholipids with nonphosphorus lipids, is a widely observed adaptive response in plants and algae. Here, we identified the transcription factor phosphorus starvation response 1 (NoPSR1) as an indispensable player …

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Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES-2145

雅子 岩井, Masako Iwai, Shinsuke Shimizu, S. Shimizu et autres