Charlotte De Bruyn

Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds in Cichorium intybus

Charlotte De Bruyn, Tom Ruttink, Elia Lacchini, Stéphane Rombauts et autres

Industrial chicory (Cichorium intybus var. sativum) and witloof (C. intybus var. foliosum) are crops with an important economic value, mainly cultivated for inulin production and as a leafy vegetable, respectively. Both crops are rich in nutritionally relevant specialized metabolites with beneficial effects …

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IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction

Jonas De Saeger, Jihae Park, Kai Thoris, Charlotte De Bruyn et autres

BACKGROUND: Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amounts of high-quality genomic DNA. Other PCR-based techniques are either inaccurate, laborious, …

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Establishment of CRISPR/Cas9 Genome Editing in Witloof (Cichorium intybus var. foliosum)

Charlotte De Bruyn, Tom Ruttink, Tom Eeckhaut, Thomas B. Jacobs et autres

Cichorium intybus var. foliosum (witloof) is an economically important crop with a high nutritional value thanks to many specialized metabolites, such as polyphenols and terpenoids. However, witloof plants are rich in sesquiterpene lactones (SL) which are important for plant defense but also …

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Successful protoplast transfection in Cichorium

Charlotte De Bruyn, Katrijn Van Laere, Ellen De Keyser, Tom Eeckhaut

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CRISPR/Cas9 to modify bitterness in Cichorium intybus

Charlotte De Bruyn, Tom Ruttink, Ellen De Keyser, Tom Eeckhaut et autres

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