Charles Winterhalter

Bacillus subtilis DnaB forms multiple protein–protein interactions essential for DNA replication initiation

Aurélie Guyet, Reyes Ruiz Campoy, Petra MANJA, Frederic D. Schramm et autres

DNA replication is initiated at specific chromosomal loci termed origins. In bacteria, the master replication initiation protein DnaA unwinds the origin (oriC), allowing a pair of replicative helicases to be loaded around each strand of the DNA duplex. The molecular mechanisms for …

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Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading

Charles Winterhalter, Kathryn J. Stratton, Stepan Fenyk, Heath D. Murray

DNA damage occurs in all cells and must be repaired to maintain genome integrity. Many DNA lesions are targeted for removal by repair systems that excise the damage, thereby generating a temporary single-strand discontinuity in the chromosome. If DNA repair has not …

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Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria

Hannah Gaimster, Daniel Stevens, James W. Grimshaw, Julia Hubbard et autres

Cellular proliferation relies on the successful coordination and completion of genome replication and segregation. To help achieve this, many bacteria utilize regulatory pathways that ensure DNA replication initiation only occurs once per cell cycle. When dysregulated, loss of DNA replication control can …

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Rescuing bacterial genome replication: essential functions to repair a double-strand break and restart DNA synthesis

Charles Winterhalter, Stepan Fenyk, Heath D. Murray

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Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading

Charles Winterhalter, Stepan Fenyk, Heath D. Murray

ABSTRACT DNA damage occurs in all cells and must be repaired to maintain genome integrity. Many DNA lesions are targeted for removal by repair systems that excise the damage, thereby generating a temporary single-strand discontinuity in the chromosome. If DNA repair has …

gb (code pays fourni par la source)

Rescuing the bacterial replisome at a nick: essential functions for recombinational repair and helicase reloading

Charles Winterhalter, Kathryn J. Stratton, Stepan Fenyk, Heath D. Murray

DNA damage occurs in all cells and must be repaired to maintain genome integrity. Many DNA lesions are targeted for removal by repair systems that excise the damage, thereby generating a temporary single-strand discontinuity in the chromosome. If DNA repair has not …

Rescuing the bacterial replisome at a nick: essential functions for recombinational repair and helicase reloading

Charles Winterhalter, Kathryn J. Stratton, Stepan Fenyk, Heath D. Murray

DNA damage occurs in all cells and must be repaired to maintain genome integrity. Many DNA lesions are targeted for removal by repair systems that excise the damage, thereby generating a temporary single-strand discontinuity in the chromosome. If DNA repair has not …

Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria

Hannah Gaimster, Daniel Stevens, Julia Hubbard, Katarzyna M. Mickiewicz et autres

ABSTRACT Cellular proliferation relies on successful coordination and completion of genome replication and segregation. To help achieve this, many bacteria utilise regulatory pathways that ensure DNA replication initiation only occurs once per cell cycle. When dysregulated, loss of DNA replication control can …

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The bacterial replication origin BUS promotes nucleobase capture

Simone Pelliciari, Salomé Bodet-Lefèvre, Stepan Fenyk, Daniel Stevens et autres

Abstract Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at …

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The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin

Charles Winterhalter, Simone Pelliciari, Daniel Stevens, Stepan Fenyk et autres

Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here …

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SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation

Charles Winterhalter, Daniel Stevens, Stepan Fenyk, Simone Pelliciari et autres

Bidirectional DNA replication from a chromosome origin requires the asymmetric loading of two helicases, one for each replisome. Our understanding of the molecular mechanisms underpinning helicase loading at bacterial chromosome origins is incomplete. Here we report both positive and negative mechanisms for …

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SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation

Charles Winterhalter, Daniel Stevens, Stepan Fenyk, Simone Pelliciari et autres

ABSTRACT Bidirectional DNA replication from a chromosome origin requires the asymmetric loading of two helicases, one for each replisome. Our understanding of the molecular mechanisms underpinning helicase loading at bacterial chromosome origins is incomplete. Here we report both positive and negative mechanisms …

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