Els J. M. Van Damme

Functional and Structural Characterization of the Erythrina fusca Seed Lectin EFusL: A GalNAc-Binding Lectin with Specificity for Tumor-Associated O-Glycans

Messias V. Oliveira, Francisco W. V. Martins, Clara Suarez, Valeria M. S. Ferreira et autres

Abstract Lectins from the genus Erythrina are predominantly galactoside-binding legume lectins with conserved structural features and established use as glycan-recognition tools. However, several species remain incompletely characterized, including the seed lectin from Erythrina fusca (EFusL). Here, EFusL was isolated by single-step lactose-agarose …

br, be (code pays fourni par la source)

Crystal structure, glycan specificity, and induction of cellular stress by the legume lectin DmegA

Vinicius J. S. Osterne, Lien Ervijn, Messias V. Oliveira, Vanir R. Pinto-Junior et autres

br, be, es (code pays fourni par la source)

Involvement of OsRIP 1, a ribosome-inactivating protein from rice, in plant defense against Nilaparvata lugens

Jeroen De Zaeytijd, Pengyu Chen, Freja Scheys, Kondeti Subramanyam et autres

be (code pays fourni par la source)

Guanidinylated polymers enable safe and efficient dsRNA delivery in plant cells

Sven Vereecken, Ellen Vandenbussche, Kristof De Schutter, Els J. M. Van Damme et autres

The widespread use of chemical pesticides in crop protection poses serious environmental risks and has been associated with adverse effects to human health. RNA interference (RNAi) offers a sustainable alternative by enabling species-selective gene silencing through double stranded RNA (dsRNA). However, the …

be (code pays fourni par la source)

Expression and characterization of an endo-β-1,6-galactanase from Arabidopsis thaliana

Koen Gistelinck, Xiaolan Yu, Arthur Leyder, Vinicius José Silva Osterne et autres

To date, only fungal and bacterial endo-β-1,6-galactanases from glycoside hydrolase subfamilies GH5_16 and GH30_5 have been characterized. β-1,6-galactan chains are primarily structural components of type II arabinogalactans present in plant and algal cell walls. The ATIYA1 gene, originating from Arabidopsis thaliana, was …

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Characterization of a Rice GH5_11 Gene Associated with Endosperm and Seed Traits

Koen Gistelinck, Zoë Madder, Isabel Verbeke, Els J. M. Van Damme

The plant cell wall is essential for maintaining cellular structure and regulating physiological processes such as growth and stress tolerance. Cell wall dynamics are largely mediated by cell wall-modifying enzymes, including glycoside hydrolases (GHs). In this study, we explored GH5 family members …

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Contributors

C. Aappnu, Samantha Vieira Abbad, Naglaa Abdelmoneim Abdallah, Jothi Dheivasikamani Abidharini et autres

in, Égypte, mx, Maroc, sa, kr, lb, pg, tw (code pays fourni par la source)

Regulatory small RNAs in plant–biotic stress responses

Zoë Madder, Xing Xu, Els J. M. Van Damme, Tina Kyndt

be (code pays fourni par la source)

The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins—A Case Study About Producing a Rice Enzyme

Tibo De Coninck, Hannes Vanhaeren, Els J. M. Van Damme

Biochemical characterization of proteins is fundamental to understanding their function. Typically, research in protein structure/function requires reasonable quantities of the protein of interest. Because of the low abundances in their natural environment, the heterogenous state of post-translational modifications, and the difficulty of …

be (code pays fourni par la source)

Discovery and phylogeny of a ricin-B-like domain from rice

Tibo De Coninck, Pierre Rougé, Els J. M. Van Damme

Lectins are carbohydrate-binding proteins which play key roles in various biological processes, including cell signaling, pathogen recognition and development. Previous research conducted on ricin-B lectin domains and carbohydrate-binding modules of family 13 (CBM13) illustrated the striking resemblances between these two groups of …

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The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins—A Case Study About Producing a Rice Enzyme

Tibo De Coninck, Hannes Vanhaeren, Els J. M. Van Damme

Biochemical characterization of proteins is fundamental to understanding their function. Typically, research in protein structure/function requires reasonable quantities of the protein of interest. Because of low abundance in their natural environment, heterogenous state of post-translational modifications or difficulty in obtaining the tissue …

be (code pays fourni par la source)

OsAPSE modulates non-covalent interactions between arabinogalactan protein O-glycans and pectin in rice cell walls

Tibo De Coninck, Isabel Verbeke, Pierre Rougé, Tom Desmet et autres

Flexibility of cell walls is crucial to accommodate cell elongation and growth, typically associated with the reorganization of cell wall polysaccharides. Seed germination is a fast-paced developmental process in which cell wall adaptability is highly required. The plant cell utilizes multiple strategies …

be, fr (code pays fourni par la source)