Christopher M. West

Glycocalyx‐Driven Immunomodulation in Trypanosoma cruzi : Structures, Host Responses, Diagnostic and Therapeutic Targeting

Norton Heise, Christopher M. West, Carolina Macedo Koeller

Chagas disease, caused by Trypanosoma cruzi, remains an important neglected infection due to underdiagnosis, global spread resulting from increased migration of people infected with T. cruzi and concomitant organ transplantation and blood transfusion, and limited availability of chemotherapy. The parasite's surface is …

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Potential for the Terminal SKP1 Glycosyltransferase to Exert Non-Enzymatic Control of SKP1 in Toxoplasma gondii

Donovan A. Cantrell, Elisabet Gas‐Pascual, Christopher M. West

The SKP1/CUL1/F-Box protein (SCF) family of E3 ubiquitin ligases is responsible for targeting multiple proteins for degradation by the 26S proteosome. Within the family, multiple F-box proteins (FBPs) associate with the SKP1 adaptor which in turn docks to CUL1. In the human …

Protist oxygen-sensing and the role of prolyl hydroxylase-regulation of E3(SCF) ubiquitin ligases

Donovan A. Cantrell, Ira J. Blader, Christopher M. West

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Global characterization of Dictyostelium discoideum gene and protein expression changes under hypoxic conditions

Julie Hesnard, Elisabet Gas‐Pascual, Hanke van der Wel, Olivier Cochet‐Escartin et autres

Aerobic eukaryotes utilize O 2 to oxidize metabolites and generate ATP. The response to oxygen deprivation leads to migration to locations with more favorable concentrations (aerotaxis), differentiation, metabolic reprogramming, or more global stress responses. In mammals, changes in gene expression are particularly …

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Glycoregulation of E3(SCF) ubiquitin ligases in unicellular eukaryotes

Hanke van der Wel, Christopher M. West

Skp1 is an essential adaptor within the Skp1/Cul1/F-box (SCF) class of E3 polyubiquitin ligases that regulate protein degradation in all eukaryotes. Skp1 is also a target of a 5-enzyme glycosylation pathway in parasites and other unicellular eukaryotes. Glycosylation of Skp1 is contingent …

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O-fucosylation affects abundance but not localization of select nucleocytoplasmic proteins in toxoplasma gondii

Megna Tiwari, Elisabet Gas‐Pascual, Janice Teal-Urquides, John C. Samuelson et autres

Toxoplasma gondii is a highly successful intracellular mammalian and avian pathogen that must adapt to a wide range of intracellular and extracellular environments. A mechanism that may support this is the modification of hydroxyamino acid rich sequences of nucleocytoplasmic proteins with O-fucose. …

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Glycosylation Weakens Skp1 Homodimerization in Toxoplasma gondii by Interrupting a Fuzzy Interaction

Ramona J. Bieber Urbauer, Hyun Woo Kim, Robert J. Woods, Jeffrey L. Urbauer et autres

High Resolution Image Download MS PowerPoint Slide Skp1/Cullin-1/F-Box protein (SCF) complexes represent a major class of E3 ubiquitin ligases responsible for proteomic control throughout eukaryotes. Target specificity is mediated by a large set of F-box proteins (FBPs) whose F-box domains interact with …

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Global characterization of Dictyostelium discoideum gene expression changes under hypoxic conditions

Julie Hesnard, Elisabet Gas‐Pascual, Hanke van der Wel, Olivier Cochet‐Escartin et autres

Abstract Aerobic eukaryotes utilize O 2 to oxidize metabolites and generate ATP. The protist kingdom lacks the Hypoxia Inducible Factor-dependent transcriptional response network to accommodate low O 2 though possesses a HIF prolyl hydroxylase that initially mediates the response. To address the …

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PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages

Sreelakshmi K. Sreenivasamurthy, Carlos Gustavo Baptista, Christopher M. West, Ira J. Blader et autres

Plasmodium species replicate via schizogony, which involves asynchronous nuclear divisions followed by semi-synchronous segmentation and cytokinesis. Successful segmentation requires a double-membranous structure known as the inner membrane complex (IMC). Here we demonstrate that PfFBXO1 (PF3D7_0619700) is critical for both asexual segmentation and …

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Novel antibodies detect nucleocytoplasmic O-fucose in protist pathogens, cellular slime molds, and plants

Megna Tiwari, Elisabet Gas‐Pascual, Manish Goyal, Marla Popov et autres

ABSTRACT Cellular adaptations to change often involve post-translational modifications of nuclear and cytoplasmic proteins. An example found in protists and plants is the modification of serine and threonine residues of dozens to hundreds of nucleocytoplasmic proteins with a single fucose (O-fucose). A …

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Toxoplasma chitinase-like protein orchestrates cyst wall glycosylation to facilitate effector export and cyst turnover

Yong Fu, Tadakimi Tomita, Louis M. Weiss, Christopher M. West et autres

bradyzoites reside in tissue cysts that undergo cycles of expansion, rupture, and release to foster chronic infection. The glycosylated cyst wall acts as a protective barrier, although the processes responsible for formation, remodeling, and turnover are not understood. Herein, we identify a …

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Novel antibodies detect nucleocytoplasmic O-fucose in protist pathogens, cellular slime molds, and plants

Megna Tiwari, Elisabet Gas‐Pascual, Manish Goyal, Marla Popov et autres

ABSTRACT Cellular adaptations to change often involve post-translational modifications of nuclear and cytoplasmic proteins. An example found in protists and plants is the modification of serine and threonine residues of dozens to hundreds of nucleocytoplasmic proteins with a single fucose (O-Fuc). A …

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