Angela Tarver

Probing the limits of genetic recoding using multi-omics-guided evolution

Ákos Nyerges, Anush Chiappino-Pepe, Bogdan Budnik, Maximilien S. Baas-Thomas et autres

Engineering the genetic code—by reassigning multiple of the 64 natural codons—enables making organisms resistant to all viruses, preventing genetic information exchange, and allowing the biosynthesis of genetically encoded unnatural polymers. However, synonymous codon replacement—recoding—is frequently lethal, and how recoding impacts fitness remains …

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Advancing Protein Display on Bacterial Spores through an Extensive Survey of Coat Components

Todd C. Chappell, Trevor B. Nicks, Jessica A. Lee, Jan‐Fang Cheng et autres

ABSTRACT The profound stability of bacterial spores makes them a promising platform for biotechnological applications like biocatalysis, bioremediation, drug delivery, etc. However, though the Bacillus subtilis spore is composed of >40 proteins, only ∼12 have been explored as fusion carriers for protein …

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Advancing Protein Display on Bacterial Spores Through an Extensive Survey of Coat Components

Todd C. Chappell, Trevor B. Nicks, Jan‐Fang Cheng, Angela Tarver et autres

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Synthetic genomes unveil the effects of synonymous recoding

Ákos Nyerges, Anush Chiappino-Pepe, Bogdan Budnik, Maximilien S. Baas-Thomas et autres

Abstract Engineering the genetic code of an organism provides the basis for (i) making any organism safely resistant to natural viruses and (ii) preventing genetic information flow into and out of genetically modified organisms while (iii) allowing the biosynthesis of genetically encoded …

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Dissolved Inorganic Carbon-Accumulating Complexes from Autotrophic Bacteria from Extreme Environments

Sarah M. Schmid, Dale Chaput, Mya Breitbart, Rebecca A. Hines et autres

To facilitate the synthesis of biomass from CO 2 , autotrophic organisms use a variety of mechanisms to increase intracellular DIC concentrations. A novel type of multisubunit complex has recently been described, which has been shown to generate measurably elevated intracellular DIC …

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Aerobic bacterial methane synthesis

Qian Wang, Abdullah Mohammed Alowaifeer, Patricia Kerner, Narayanaganesh Balasubramanian et autres

Significance Observations summarized herein contribute to an ongoing paradigm shift in microbial ecology, documenting an emergent property of ecosystem function that further challenges the perception that biogenic methane (CH 4 ) production is strictly an anaerobic process. Relevant metabolites, a model bacterial …

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An Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology

Ernst Oberortner, Robert S. Evans, Xianwei Meng, Sangeeta Nath et autres

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Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere

Devin F. R. Doud, Robert M. Bowers, Frederik Schulz, Markus de Raad et autres

Assigning a functional role to a microorganism has historically relied on cultivation of isolates or detection of environmental genome-based biomarkers using a posteriori knowledge of function. However, the emerging field of function-driven single-cell genomics aims to expand this paradigm by identifying and …

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A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides

Luísa Czamanski Nora, Maren Wehrs, Joonhoon Kim, Jan‐Fang Cheng et autres

BACKGROUND: Rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and resources. However, there is a lack of characterized promoters to drive expression …

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Rare earth element alcohol dehydrogenases widely occur among globally distributed, numerically abundant and environmentally important microbes

Jing Huang, Yu Zheng, Joseph D. Groom, Jan‐Fang Cheng et autres

Abstract Lanthanides (Ln3+), known as rare earth elements, have recently emerged as enzyme cofactors, contrary to prior assumption of their biological inertia. Several bacterial alcohol dehydrogenases have been characterized so far that depend on Ln3+ for activity and expression, belonging to the …

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A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides

Luísa Czamanski Nora, Maren Wehrs, Joonhoon Kim, Jan‐Fang Cheng et autres

ABSTRACT Background Rhodosporidium toruloides is a promising host for the production of bioproducts from lignocellulosic biomass. A key prerequisite for efficient pathway engineering is the availability of robust genetic tools and resources. However, there is a lack of characterized promoters to drive …

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An integrated workflow for phenazine-modifying enzyme characterization

Roger Coates, Benjamin P. Bowen, Ernst Oberortner, Linda S. Thomashow et autres

Increasing availability of new genomes and putative biosynthetic gene clusters (BGCs) has extended the opportunity to access novel chemical diversity for agriculture, medicine, environmental and industrial purposes. However, functional characterization of BGCs through heterologous expression is limited because expression may require complex …

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