Michisato Toyoda

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue

Takao Yasuda, Yoichi Otsuka, Tasuku Kato, Shuichi Shimma et autres

To elucidate disease mechanisms, it is essential to develop analytical techniques capable of visualizing cellular heterogeneity in biological tissues. Mass spectrometry imaging (MSI) allows simultaneous visualization of multiple biomolecules in tissue sections in a single measurement. However, achieving single-cell (SC)-MSI requires high …

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Instrumental Dispersion Evaluation on Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Tandem Mass Spectrometry

Toshinobu Hondo, Yumi Miyake, Michisato Toyoda

Instrumental dispersion in the ion source can severely distort fast chromatographic peaks in supercri cal fluid chromatography (SFC)-mass spectrometry (MS).Despite this importance, the dispersion characteris cs specific to medium-vacuum chemical ioniza on (MVCI) sources have not been quan ta vely inves gated.In …

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Algorithm-Driven Chromatographic Method for Prostaglandin Isomer Identification via Tandem Mass Spectrometry

Toshinobu Hondo, Yumi Miyake, Michisato Toyoda

This study explores the computational isolation of prostaglandin (PG) isomers, specifically PG E 2 (PGE 2 ) and D 2 (PGD 2 ), to enhance method development efficiency and provide insights into their retention behavior during supercritical fluid extraction (SFE) combined with …

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Comparative study of ablation processes using femtosecond and nanosecond lasers

Yasuo Kanematsu, Atsushi Muratsugu, Fukuhisa Matsuda, Wakana Matsuda et autres

This study investigated the dynamic characteristics of ions emitted from materials subjected to short-pulsed laser interactions. To investigate the difference in ablation dynamics due to laser pulse irradiation with the same energy but different time scales, we developed a time-of-flight (TOF) mass …

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Mass Spectrometry Imaging of Time-Dependently Photodegraded Light Stabilizers in Polyethylene Films Using Tapping-Mode Scanning Probe Electrospray Ionization

Tsuyoshi Akiyama, Yoichi Otsuka, Mengze Sun, Shinichi Yamaguchi et autres

Light stabilizers are additives that are widely used to improve the lifespan and performance of polymer materials. To develop advanced polymer materials, analytical techniques investigate the degradation mechanisms and distribution of additives in polymers are crucial. Herein, two extraction-ionization methods were used: …

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Single-cell mass spectrometry imaging of lipids in HeLa cells via tapping-mode scanning probe electrospray ionization

Yoichi Otsuka, Kazuya Kabayama, Ayane Miura, Masatomo Takahashi et autres

The development of analytical technology that allows investigation of the diversity of cells that form biological tissues based on molecular information is important to elucidate the heterogeneity of cells and pathological mechanisms. Here, we present a proof-of-concept demonstration of single-cell mass spectrometry …

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Impact of Spin-Coating Temperature on Morphology of Pb-Based Mixed Ion Perovskite Films and Their Solar Cell Performance

Wakana Matsuda, Keishiro Goshima, Insub Noh, Hyung Do Kim et autres

Performance of metal halide perovskite solar cells depends largely on the perovskite film quality. Here, we demonstrate the impact of spin-coating temperature under anhydrous conditions on Pb-based mixed ion perovskite film morphology, crystal structure, optical properties, and their solar cell performance. The …

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Comparative Study of Ablation Processes Using Femtosecond and Nanosecond Lasers

Jianzhong Wang, Yasuo Kanematsu, Atsushi Muratsugu, Fuyuki Matsuda et autres

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Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes

Yoichi Otsuka, Maki Okada, Tomomi Hashidate‐Yoshida, Katsuyuki Nagata et autres

Mass spectrometry imaging (MSI) is a promising analytical method to visualize the distribution of lipids in biological tissues. To clarify the relationship between cellular distribution and lipid types in a tissue, it is crucial to achieve both an improvement in ion detection …

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Rapid Analysis for α-Tocopherol and Its Oxidative Products in the Pisum sativum L. Leaf Using Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Tandem Mass Spectrometry

Toshinobu Hondo, Yumi Miyake, Michisato Toyoda

A method for the rapid determination of α-tocopherol (α-T) and its oxidative products in plant tissue has been developed using supercritical fluid extraction (SFE) coupled with supercritical fluid chromatography (SFC) and medium vacuum chemical ionization (MVCI) with tandem mass spectrometry. The method …

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Spatial extension of the transient gain drop in a microchannel plate for a single-pulse irradiation

Hiroshi Kobayashi, Toshinobu Hondo, Yasuo Kanematsu, Motohiro Suyama et autres

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A potential tumor-marker from urinary microbiota – focusing on renal cell carcinoma

Shunichi Kajioka, Atsuko Okabe, Takuro Okada, Michisato Toyoda et autres