Kenta Moriwaki

Hmgb1 release kinetics shape its extracellular functions during regulated cell death

Shin Murai, Da Young Shin, Kenta Sumiyama, Kenta Moriwaki et autres

Abstract Danger-associated molecular patterns (DAMPs), such as high mobility group protein B1 (HMGB1), are released from dying cells, yet the kinetics and functional consequences of their release remain unclear. Using Hmgb1–mCherry transgenic mice and live-cell imaging, we visualize Hmgb1 and interleukin-1β (IL-1β) …

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iPLA2β Protects Retinal Pigment Epithelium From Ferroptosis in a Sodium Iodate–Induced Model of Dry AMD

Takafumi Suzuki, Ryo Terao, Masako Nagahara, Kentarō Hayashi et autres

Purpose: Ferroptosis, characterized by lipid peroxidation, has been implicated in retinal pigment epithelium (RPE) degeneration in dry age-related macular degeneration (AMD). This study aimed to investigate the role of calcium-independent phospholipase A2 group VI (iPLA2β) in protecting RPE cells from oxidative stress …

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Dynamic movement of the Golgi unit and its glycosylation enzyme zones

Akihiro Harada, Masataka Kunii, Kazuo Kurokawa, Takuya Sumi et autres

Knowledge on the distribution and dynamics of glycosylation enzymes in the Golgi is essential for better understanding this modification. Here, using a combination of CRISPR/Cas9 knockin technology and super-resolution microscopy, we show that the Golgi complex is assembled by a number of …

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Regulation of TRAIL-induced Cancer Cell Death by Fucosylated Glycans

Kenta Moriwaki

Fucosylation is one of the most important types of glycosylation in cancer. The expression levels of fucosylated glycans generally increase during cancer development. Consequently, fucosylated molecules have been used as tumor markers. The synthesis of fucosylated glycans occurs through the catalytic activity …

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Regulation of TRAIL-induced Cancer Cell Death by Fucosylated Glycans

Kenta Moriwaki

フコシル化糖鎖は、一般的にがん化に伴い増加する糖鎖構造として知られ、フコシル化分子は腫瘍マーカーとして利用されてきた。フコシル化糖鎖の機能は多岐に渡っているが、がんの増殖や転移に関わることが知られている。フコシル化糖鎖を合成するにはフコース転移酵素のドナー基質となるGDP-フコースが必須であるが、我々はGDP-フコース合成に必須の酵素であるGDP-mannose 4,6-dehydratase（GMDS）遺伝子のがんにおける異常とそれによるGMDSタンパク質の欠損を世界に先駆けて報告してきた。また、このGMDS遺伝子異常の発見を契機に、フコシル化糖鎖が細胞死誘導分子Tumor necrosis factor-related apoptosis-inducing ligand（TRAIL）および腫瘍免疫回避機構への感受性を制御していることを明らかにしてきた。本総説では、我々のこれまでの知見を中心に、フコシル化糖鎖によるがん細胞死の制御について概説したい。

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A high-sensitivity ELISA for detection of human FGF18 in culture supernatants from tumor cell lines

Yuichi Tsuchiya, Sachiko Komazawa‐Sakon, Masato Tanaka, Takumi Kanokogi et autres

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A high-sensitivity ELISA for detection of human FGF18 in culture supernatants from tumor cell lines

Yuichi Tsuchiya, Sachiko Komazawa‐Sakon, Masato Tanaka, Takumi Kanakogi et autres

Abstract Fibroblast growth factor 18 (FGF18) is elevated in several human cancers, such as gastrointestinal and ovarian cancers, and stimulates the proliferation of tumor cells. This suggests that FGF18 may be a promising candidate biomarker in cancer patients. However, the lack of …

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Urinary pentosidine level is associated with the risk of fracture in community-dwelling older adults: a prospective observational study

Hiroshi Hagino, Kenta Moriwaki, Takashi Wada, Mari Osaki et autres

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EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblasts

Wu Ji, Kenta Moriwaki, Tatsuya Asuka, Ritsuko Nakai et autres

Cell polarity, the asymmetric distribution of proteins and organelles, is permanently or transiently established in various cell types and plays an important role in many physiological events. epidermal growth factor receptor substrate 15 homology domain-binding protein 1-like 1 (EHBP1L1) is an adapter …

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Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis

Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki et autres

Necroptosis is a regulated form of cell death involved in various pathological conditions, including ischemic reperfusion injuries, virus infections, and drug-induced tissue injuries. However, it is not fully understood when and where necroptosis occurs in vivo. We previously generated a Forster resonance …

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Lewis glycosphingolipids as critical determinants of TRAIL sensitivity in cancer cells

Tomoya Fukuoka, Kenta Moriwaki, Shinji Takamatsu, Jumpei Kondo et autres

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A FRET biosensor, SMART, monitors necroptosis in renal tubular epithelial cells in a cisplatin-induced kidney injury model

Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki et autres

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