Joseph A. Buonomo

Teaching chemistry for a more circular world

Fun Man Fung, Fabian Hollinger, Francesca M. Kerton, Mikhailey D. Wheeler et autres

Selective Probing of Acidic Amino Acid Residues for Protein Structural Analysis by Covalent Labeling and Mass Spectrometry

Akash Talukder, A Mehta, Aisosa A. Omere, Joseph A. Buonomo et autres

We present a chemical labeling strategy for specific bioconjugation of aspartic (D) and glutamic (E) acid residues, which is crucial for elucidating their roles in protein–protein interactions and potential therapeutic interventions. Traditional methods using carbodiimides are limited by nonspecificity and side-product formation, …

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Kinetic Characterization of F420-Dependent Sugar-6-Phosphate Dehydrogenase from Cryptosporangium arvum

Alaa Aziz, Desiree R. Czapski, Ravi Ramkissoon, Md Sabid Ahamed et autres

High Resolution Image Download MS PowerPoint Slide F 420 -dependent glucose-6-phosphate dehydrogenase (FGD) catalyzes the oxidation of glucose-6-phosphate (G6P) to 6-phosphogluconolactone (6PG). Recent phylogenetic analyses have identified a new subclass of these enzymes, F 420 -dependent sugar-6-phosphate dehydrogenases (FSDs), which act on …

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pH-Responsive Peptide–Polymer Hydrogel for Biofilm Disruption

Haritha Asokan‐Sheeja, Debdatta Das, Jenny N. Nguyen, Jiazhu Xu et autres

Biofilm formation presents a significant challenge in chronic infections as it enables bacteria to resist conventional antibiotics and thrive in various areas of the body. The treatment is further hurdled by the acidic environment of biofilms due to anaerobic glycolysis of bacteria …

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Peptide Sequencing Via Protein Language Models

Thuong Le Hoai Pham, Jillur Rahman Saurav, Aisosa A. Omere, Calvin J. Heyl et autres

We introduce a protein language model for determining the complete sequence of a peptide based on measurement of a limited set of amino acids. To date, protein sequencing relies on mass spectrometry, with some novel Edman degradation based platforms able to sequence …

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MmpL3, Wag31, and PlrA are involved in coordinating polar growth with peptidoglycan metabolism and nutrient availability

Neda Habibi Arejan, Desiree R. Czapski, Joseph A. Buonomo, Cara C. Boutte

ABSTRACT Cell growth in mycobacteria involves cell wall expansion that is restricted to the cell poles. The DivIVA homolog Wag31 is required for this process, but the molecular mechanism and protein partners of Wag31 have not been described. In this study of …

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Deconvolution of Human Urine across the Transcriptome and Metabolome

Sevahn K. Vorperian, Brian C. DeFelice, Joseph A. Buonomo, Hagop J. Chinchinian et autres

BACKGROUND: Early detection of the cell type changes underlying several genitourinary tract diseases largely remains an unmet clinical need, where existing assays, if available, lack the cellular resolution afforded by an invasive biopsy. While messenger RNA in urine could reflect the dynamic …

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MmpL3, Wag31 and PlrA are involved in coordinating polar growth with peptidoglycan metabolism and nutrient availability

Neda Habibi Arejan, Desiree R. Czapski, Joseph A. Buonomo, Cara C. Boutte

Abstract Cell growth in mycobacteria involves cell wall expansion that is restricted to the cell poles. The DivIVA homolog Wag31 is required for this process, but the molecular mechanism and protein partners of Wag31 have not been described. In this study of …

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Multiomics characterization of cell type repertoires for urine liquid biopsies

Sevahn K. Vorperian, Brian C. DeFelice, Joseph A. Buonomo, Hagop J. Chinchinian et autres

Urine is assayed alongside blood in medicine, yet current clinical diagnostic tests utilize only a small fraction of its total biomolecular repertoire, potentially foregoing high-resolution insights into human health and disease. In this work, we characterized the joint landscapes of transcriptomic and …

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Bioluminogenic Probe for Rapid, Ultrasensitive Detection of β-Lactam-Resistant Bacteria

Tingting Dai, Jinghang Xie, Joseph A. Buonomo, Ángel Moreno et autres

Increasingly difficult-to-treat infections by antibiotic-resistant bacteria have become a major public health challenge. Rapid detection of common resistance mechanisms before empiric antibiotic usage is essential for optimizing therapeutic outcomes and containing further spread of resistance to antibiotics among other bacteria. Herein, we …

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Cephem‐Pyrazinoic Acid Conjugates: Circumventing Resistance in Mycobacterium tuberculosis

Malcolm S. Cole, Michael D. Howe, Joseph A. Buonomo, Sachin Sharma et autres

Tuberculosis (TB) is a leading source of infectious disease mortality globally. Antibiotic-resistant strains comprise an estimated 10 % of new TB cases and present an urgent need for novel therapeutics. β-lactam antibiotics have traditionally been ineffective against M. tuberculosis (Mtb), the causative …

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Spreading of a mycobacterial cell-surface lipid into host epithelial membranes promotes infectivity

C.J. Cambier, Steven M. Banik, Joseph A. Buonomo, Carolyn R. Bertozzi

ABSTRACT Several virulence lipids populate the outer cell wall of pathogenic mycobacteria (Jackson, 2014). Phthiocerol dimycocerosate (PDIM), one of the most abundant outer membrane lipids (Anderson, 1929), plays important roles in both defending against host antimicrobial programs (Camacho et al., 2001; Cox …

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