Germán M. Traglia

Human serum albumin and biological fluids reduce cefiderocol activity in carbapenem-resistant gram-negative bacteria

Azadeh Varnado, Samyar Moheb, Shayra D. Sanchez, Cecilia Rodriguez et autres

Human serum albumin (HSA), the predominant plasma protein, influences bacterial physiology and antibiotic susceptibility. We previously showed that HSA exposure alters Acinetobacter baumannii transcriptional profiles, reducing biofilm formation and modulating the expression of β-lactam resistance genes, while decreasing cefiderocol (FDC) activity through …

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Two Cases of Posttraumatic Kosakonia Infection, Argentina, 2023

Claudia Barberis, María Sol Haim, Paula N. Zomero, Germán M. Traglia et autres

We describe 2 plant-associated posttraumatic Kosakonia infections in Argentina. Facing biochemical and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry limitations, we used whole-genome sequencing to successfully identify K. cowanii and K. oryzae as the causative agents. Our data highlight the crucial role of …

Nutrient-Driven Small-Colony Variants as an Adaptive Outcome in Carbapenem-Resistant Acinetobacter baumannii

Germán M. Traglia, María Soledad Ramirez

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a serious clinical threat due to its remarkable adaptability and emerging resistance to antibiotics of last resort such as cefiderocol. There is growing evidence that host-mimicking nutrient conditions can significantly modulate antibiotic susceptibility, but the contribution …

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Nutrient-Driven Small-Colony Variants as an Adaptive Outcome in Carbapenem-Resistant Acinetobacter baumannii

Germán M. Traglia, María Soledad Ramirez

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a serious clinical threat due to its remarkable adaptability and emerging resistance to antibiotics of last resort such as cefiderocol. There is growing evidence that host-mimicking nutrient conditions can significantly modulate antibiotic susceptibility, but the contribution …

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Vitamin B12 promotes cefiderocol resistance and small-colony variants in carbapenem-resistant Acinetobacter baumannii

Vyanka Mezcord, Irene Luu, Usman Akhar, Germán M. Traglia et autres

ABSTRACT Carbapenem-resistant Acinetobacter baumannii (CRAB) is a global health threat with few effective treatment options remaining. Cefiderocol, a last-resort siderophore-cephalosporin antibiotic, exploits bacterial iron transport systems via TonB-dependent receptors (TBDRs) to gain cellular entry. However, treatment failures and the emergence of resistance …

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P-1281. Genomic and Transcriptional Adaptations in a Spontaneous Cefiderocol-Resistant Klebsiella pneumoniae KPC-Producing Mutant Isolate

Irene Luu, Vyanka Mezcord, Jenny Escalante, Germán M. Traglia et autres

Abstract Background Carbapenem-resistant Klebsiella pneumoniae CRKP) represents a critical public health threat, with limited treatment options due to its resistance to last-line antibiotics. Cefiderocol (FDC), a novel siderophore cephalosporin, has shown efficacy against CRKP; however, resistance has emerged. This study characterizes a …

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Nutrient-Driven Small-Colony Variants as an Adaptive Outcome in Carbapenem-Resistant Acinetobacter baumannii

Samyar Moheb, Shayra D. Sanchez, Irene Luu, Azadeh Varnado et autres

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Functional and population-genomic dissection of rifabutin response in carbapenem-resistant Acinetobacter baumannii

Germán M. Traglia, María Soledad Ramirez

Rifamycins, and rifabutin (RFB) in particular, have re-emerged as candidate agents against carbapenem-resistant Acinetobacter baumannii (CRAB), with previous data proposing the siderophore receptor FhuE as the principal route of RFB uptake. Here we combined targeted mutant screening, killing-dynamics assays, scanning electron microscopy …

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Functional and population-genomic dissection of rifabutin response in carbapenem-resistant Acinetobacter baumannii

Germán M. Traglia, María Soledad Ramirez

Rifamycins, and rifabutin (RFB) in particular, have re-emerged as candidate agents against carbapenem-resistant Acinetobacter baumannii (CRAB), with previous data proposing the siderophore receptor FhuE as the principal route of RFB uptake. Here we combined targeted mutant screening, killing-dynamics assays, scanning electron microscopy …

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Ampicillin/Sulbactam in Combination with Ceftazidime/Avibactam Against Metallo-β-Lactamase-Producing Carbapenem-Resistant Acinetobacter baumannii : A Genomics-Informed Mechanism-based model

Bhavatharini Arun, Eloise Liu, Quentin Vallé, Rajnikant Sharma et autres

BACKGROUND: Carbapenem-resistant Acinetobacter baumannii (CRAB) infections, driven by metallo- and class-D serine β-lactamases, pose significant therapeutic challenges. β-Lactam-based combinations offer a promising alternative to agents like colistin and tigecycline, which are associated with toxicity, and to cefiderocol, which is facing emerging resistance. …

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Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance

Usman Akhtar, Samyar Moheb, Carol Davies‐Sala, Fernando Pasterán et autres

The emergence of antimicrobial resistance in Acinetobacter species poses a significant clinical challenge, particularly in non -baumannii species, which are often overlooked in healthcare settings. In this study, we characterized two Acinetobacter clinical isolates, AMA204 and AMA207—identified as A. junii and A. …

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Spontaneous Emergence of Cefiderocol Resistance in Klebsiella pneumoniae KPC-163: Genomic and Transcriptomic Insights

Irene Luu, Vyanka Mezcord, Jenny Escalante, Germán M. Traglia et autres

Background/Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an urgent public health threat due to its rapid dissemination and resistance to last-line antibiotics. Cefiderocol (FDC), a novel siderophore cephalosporin, targets resistant Gram-negative pathogens by exploiting bacterial iron uptake mechanisms. However, resistance to FDC is …

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