Zhaojuan Zheng

Bridging industrial pretreatment and downstream bioconversion through low-enzyme high-solids ethanol production from unwashed wheat straw

Yuyang Fan, Rou Weng, Yuanjia Gu, Rong Zhang et autres

High-solids enzymatic hydrolysis is essential for producing fuel ethanol from pretreated wheat straw at industrially relevant titers, but it is limited by high enzyme demand, poor slurry mobility, lignin-related inhibition, and soluble inhibitors retained in unwashed biomass. This study aimed to develop …

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Expanding Aldopentose Catalysis of a Novel PQQ-Dependent Glucose Dehydrogenase without Compromising Native d -Glucose Activity

Yuehua Chen, Peng Liu, Binbin Sheng, Jiaxin Xing et autres

Lignocellulosic biomass is an abundant renewable resource that is rich in aldohexoses and aldopentoses. Efficient biocatalysts for aldopentose oxidation remain limited, and few enzymes exhibit high catalytic efficiency toward both aldohexoses and aldopentoses. Here, a naturally promiscuous pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) …

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High-level de novo biosynthesis of plant-derived pinosylvin in Yarrowia lipolytica via metabolic engineering and surfactant-mediated fermentation

Huanghong Tan, Yuxuan Wu, X M Chen, Yuanming Tao et autres

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Efficient one-pot lactic acid production from cellulose via a Trichoderma reesei and Bacillus coagulans cooperation leveraging substrate-induced secretomes

Shuai Liang, Chang Yu, Jiaming Fu, Yuanming Tao et autres

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A synthetic biology toolkit for the plasmid-dependent and thermophilic methylotroph Bacillus methanolicus

Pan Liu, Qianqian Yuan, Xueting Yang, Qian Wang et autres

Bacillus methanolicus, a unique plasmid-dependent and thermophilic methylotroph, is an ideal chassis for one-carbon (C1) biomanufacturing. Despite its evolutionary uniqueness and industrial promise, the synthetic biology toolkit remains limited in comparison to that of conventional model microorganisms. Here, we present a comprehensive …

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Insight into the enzymatic digestion limitation of wheat straw after diluted lactic acid pretreatment from the perspective of residual lignin

Hongxin Yu, Yijie Xu, Xiaohu Jin, Jie Shan Hou et autres

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Corrigendum to “Development of a sustainable approach to produce galactaric acid from sunflower heads by engineered Pseudomonas putida KT2440” [Bioresour. Technol. 437 (2025) 133115]

Huanghong Tan, Jie Hou, Jia Ouyang, Zhaojuan Zheng

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Development of a sustainable approach to produce galactaric acid from sunflower heads by engineered Pseudomonas putida KT2440

Huanghong Tan, Jie Hou, Jia Ouyang, Zhaojuan Zheng

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Unlocking corn stover for high utilization of fermentable sugars from both cellulose and hemicellulose: combining alkaline sodium sulfite and diluted citric acid pretreatment

Hongxiao Li, Jie Hou, Hongxin Yu, Xiaoli Gu et autres

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A synthetic biology toolkit for interrogating plasmid-dependent methylotrophy and enhancing methanol-based biosynthesis of Bacillus methanolicus

Pan Liu, Qianqian Yuan, Xueting Yang, Qian Wang et autres

Abstract Bacillus methanolicus represents a thermophilic methylotroph whose methanol utilization depends on plasmid-encoded genes. It serves as a unique model for deciphering plasmid-dependent methylotrophy and an ideal chassis for low-carbon biomanufacturing using CO2-derived C1 substrates. Despite its evolutionary uniqueness and industrial potential, …

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Engineering the production of the wood stilbene compound pinosylvin from lignin-derived trans-cinnamic acid in Escherichia coli by modulating malonyl-CoA pathway

Xiaoxia Li, Huanghong Tan, Zijie J. Wang, Zhaojuan Zheng et autres

Pinosylvin, a valuable stilbene compound found in Pinus heartwood, exhibits various bioactivities including antimicrobial, anti-inflammatory, and antitumor effects. Currently, pinosylvin is mostly obtained by direct plant extraction from woody plants, which is not environmentally friendly and difficult to meet the demand for …

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Synergistic boosting furoic acid and glucose production from corn stover by tandem chemocatalysis and biocatalysis

Lirong Huang, Yuehua Chen, Jia Ouyang, Zhaojuan Zheng

The synthesis of bio-based compounds derived from biomass is essential for promoting sustainable development and environmental preservation. In this study, a tandem chemocatalysis and biocatalysis approach was proposed to co-produce furoic acid (FA) and glucose from corn stover. The corn stover was …

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