Rhodel K. Simbulan

Self-reinforcing ROS-aneuploidy feedback loop shapes preimplantation development

Seok Hee Lee, Andrew Alamban, Rhodel K. Simbulan, Philip Marsh et autres

ABSTRACT Aneuploidy and oxidative stress have each been implicated in impaired embryonic development, yet their relationship remains unclear. Here we discover a self-reinforcing ROS-aneuploidy feedback loop with hysteretic properties in preimplantation development. In human blastocysts, oxidative stress associates with aneuploidy at the …

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P-200 Beyond Appearance: Oxidative Stress in Human Embryos and Its Hidden Connection to Aneuploidy

Paolo F. Rinaudo, S H Lee, Rhodel K. Simbulan, Phil J. Marsh et autres

Abstract Study question Does oxidative stress differ between euploid and aneuploid human embryos, and is it associated with embryo morphology, developmental timing, maternal age, or fertilization method? Summary answer Human euploid blastocysts exhibit lower oxidative damage than aneuploid ones, independent of maternal …

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Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice

Rhodel K. Simbulan, Seok Hee Lee, Reza K. Oqani, Xiaowei Liu et autres

Assisted reproductive technologies (ARTs) have rapidly evolved since being introduced in 1978. However, many of the procedures used clinically lack a basis of long-term studies to ensure their safety. Though in vitro fertilization (IVF) is largely safe, follow-up studies have shown that …

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WITHDRAWN: A proof-of-concept prospective study of applying artificial intelligence for sperm selection in the IVF laboratory

Kiran Joshi, Rhodel K. Simbulan, Anthony M. Rajah, Gregory Burd et autres

A proof of concept for a deep learning system that can aid embryologists in predicting blastocyst survival after thaw

Phil J. Marsh, Dahlia Radif, Pranav Rajpurkar, Z Wang et autres

The ability to understand whether embryos survive the thaw process is crucial to transferring competent embryos that can lead to pregnancy. The objective of this study was to develop a proof of concept deep learning model capable of assisting embryologist assessment of …

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A Proof of Concept for a Deep Learning System that Can Aid Embryologists in Predicting Blastocyst Survival After Thaw

Philip J. Marsh, Dahlia Radif, Pranav Rajpurkar, Zihan Wang et autres

Abstract The ability to understand whether embryos survive the thaw process is crucial to transferring competent embryos that can lead to pregnancy. The objective of this study was to develop a deep learning model capable of assisting embryologist assessment of survival of …

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A deep dive into the morphokinetics and ploidy of low-quality blastocysts

Molly M. Quinn, Philip J. Marsh, Salustiano Ribeiro, Rhodel K. Simbulan et autres

Objective: To describe morphokinetic parameters and ploidy among low-quality blastocysts not meeting the criteria for clinical use. Design: Prospective cohort study. Setting: Academic medical center. Patients: Two hundred patients undergoing in vitro fertilization between February 2018 and November 2019. Interventions: All embryos …

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Microfluidic preparation of spermatozoa for ICSI produces similar embryo quality to density-gradient centrifugation: a pragmatic, randomized controlled trial

Molly M. Quinn, Salustiano Ribeiro, Flor Juarez‐Hernandez, Rhodel K. Simbulan et autres

STUDY QUESTION: Does processing of spermatozoa for IVF with ICSI by a microfluidic sperm separation device improve embryo quality compared with density-gradient centrifugation? SUMMARY ANSWER: Patients randomized to microfluidic sperm preparation had similar cleavage- and blastocyst-stage embryo quality and clinical and ongoing …

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Aneuploidy rates and morphokinetic parameters of embryos cultured in distinct culture media: a sibling oocyte study

Molly M. Quinn, Philip J. Marsh, Salustiano Ribeiro, Rhodel K. Simbulan et autres

STUDY QUESTION: Do embryos from sibling oocytes assigned to distinct single-step media culture systems demonstrate differences in early embryo development, morphokinectics or aneuploidy rates? SUMMARY ANSWER: Embryo quality, morphokinetic parameters and aneuploidy rates from trophectoderm biopsy were similar between sibling embryos cultured …

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Conception after chemotherapy: post-chemotherapy method of conception and pregnancy outcomes in breast cancer patients

Mary Kathryn Abel, Kaitlyn Wald, Nikita Sinha, Joseph M. Letourneau et autres

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Diminished ovarian reserve is associated with reduced euploid rates via preimplantation genetic testing for aneuploidy independently from age: evidence for concomitant reduction in oocyte quality with quantity

Eleni A. Greenwood, Charles E. McCulloch, Rhodel K. Simbulan, Marcelle Ivonne Cedars et autres

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Effect of culture conditions and method of conception on mouse live birth rate

Zhuoni Xiao, Annemarie A. Donjacour, Royce Harner, Rhodel K. Simbulan et autres

OBJECTIVE: To understand in a mouse model whether there are differences in the decidua and live birth rate after transfer of blastocysts generated by in vitro fertilization (IVF) or by superovulation with spontaneous mating into unstimulated recipients. DESIGN: Animal experiment. SETTING: University-affiliated …

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