Raphaël Thuret

Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe

Tommaso Ristori, Raphaël Thuret, Erika Hooker, Peter Quicke et autres

Sprouting angiogenesis and blood vessel stabilization require precise coordination between endothelial cells (ECs) and pericytes. Bone Morphogenic Protein 9 (Bmp9), whose signaling through activin receptor-like kinase 1 (Alk1) is dysregulated in several diseases, was thought to regulate these processes by independently activating …

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Biomechanical Studies with Xenopus Animal Caps: A 3D-Printed Mechanical Bracket for Maintaining Tissue Stretch During Immunofluorescence Analysis

Irwin Phanada, Raphaël Thuret, Sarah Woolner

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Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe

Tommaso Ristori, Raphaël Thuret, Erika Hooker, Peter Quicke et autres

In brief: The mechanisms regulating the signaling pathways involved in angiogenesis are not fully known. Ristori et al. show that Lunatic Fringe (LFng) mediates the crosstalk between Bone Morphogenic Protein 9 (Bmp9) and Notch signaling, thereby regulating the endothelial cell behavior and …

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Foxm1 regulates neural progenitor fate during spinal cord regeneration

Diane Pelzer, Lauren S. Phipps, Raphaël Thuret, Carlos J. Gallardo-Dodd et autres

Abstract Xenopus tadpoles have the ability to regenerate their tails upon amputation. Although some of the molecular and cellular mechanisms that globally regulate tail regeneration have been characterised, tissue-specific response to injury remains poorly understood. Using a combination of bulk and single-cell …

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Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and in vivo

Bahti Zakirov, Georgios Charalambous, Raphaël Thuret, Irene M. Aspalter et autres

Abstract How do cells make efficient collective decisions during tissue morphogenesis? Humans and other organisms use feedback between movement and sensing known as ‘sensorimotor coordination’ or ‘active perception’ to inform behaviour, but active perception has not before been investigated at a cellular …

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Active Perception during Angiogenesis: Filopodia speed up Notch selection of tip cells in silico and in vivo

Bahti Zakirov, Georgios Charalambous, Irene M. Aspalter, Kelvin Van-Vuuren et autres

Abstract How do cells make efficient collective decisions during tissue morphogenesis? Humans and other organisms utilize feedback between movement and sensing known as ‘sensorimotor coordination’ or ‘active perception’ to inform behaviour, but active perception has not before been investigated at a cellular …

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Foxm1 regulates neuronal progenitor fate during spinal cord regeneration

Diane Pelzer, Lauren S. Phipps, Raphaël Thuret, Syed Murtuza Baker et autres

Summary Mammals have limited tissue regeneration capabilities, particularly in the case of the central nervous system. Spinal cord injuries are often irreversible and lead to the loss of motor and sensory function below the site of the damage [1]. In contrast, amphibians …

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Positive Feedback Defines the Timing, Magnitude, and Robustness of Angiogenesis

Donna J. Page, Raphaël Thuret, Lakshmi Venkatraman, Tokiharu Takahashi et autres

Angiogenesis is driven by the coordinated collective branching of specialized leading "tip" and trailing "stalk" endothelial cells (ECs). While Notch-regulated negative feedback suppresses excessive tip selection, roles for positive feedback in EC identity decisions remain unexplored. Here, by integrating computational modeling with …

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Positive-feedback defines the timing and robustness of angiogenesis

Donna J. Page, Raphaël Thuret, Lakshmi Venkatraman, Tokiharu Takahashi et autres

SUMMARY Blood vessel formation by angiogenesis is critical for tissue development, homeostasis and repair, and is frequently dysregulated in disease[1–3]. Angiogenesis is triggered by vascular endothelial growth factor receptor-2/3 (VEGFR) signalling, which induces motile endothelial cell (ECs) tip identity[4,5]. Tip cells lead …

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Analysis of neural progenitors from embryogenesis to juvenile adult inXenopus laevisreveals biphasic neurogenesis and continuous lengthening of the cell cycle

Raphaël Thuret, Hélène Auger, Nancy Papalopulu

Xenopus laevis is a prominent model system for studying neural development, but our understanding of the long-term temporal dynamics of neurogenesis remains incomplete. Here, we present the first continuous description of neurogenesis in X. laevis, covering the entire period of development from …

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aPKC Phosphorylates p27Xic1, Providing a Mechanistic Link between Apicobasal Polarity and Cell-Cycle Control

Nitin Sabherwal, Raphaël Thuret, Robert William Lea, Peter J. Stanley et autres

During the development of the nervous system, apicobasally polarized stem cells are characterized by a shorter cell cycle than nonpolar progenitors, leading to a lower differentiation potential of these cells. However, how polarization might be directly linked to the kinetics of the …

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Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging

Hua Tian, John S. Fletcher, Raphaël Thuret, Alex Henderson et autres

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging has been used for the direct analysis of single intact Xenopus laevis embryo surfaces, locating multiple lipids during fertilization and the early embryo development stages with subcellular lateral resolution (∼4 μm). The method avoids the …

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