Masashi Takahara

Comprehensive immunotherapy combined with intratumoral injection of zoledronate-pulsed dendritic cells, intravenous adoptive activated T lymphocyte and gemcitabine in unresectable locally advanced pancreatic carcinoma: a phase I/II trial

Yoshiki Hirooka, Hiroki Kawashima, Eizaburo Ohno, Takuya Ishikawa et autres

// Yoshiki Hirooka 1 , Hiroki Kawashima 2 , Eizaburo Ohno 2 , Takuya Ishikawa 2 , Takashi Kamigaki 3, 4 , Shigenori Goto 3 , Masashi Takahara 5 and Hidemi Goto 2 1 Department of Endoscopy, Nagoya University Hospital, Nagoya, Japan …

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Dendritic cell vaccines loaded with autologous tumor lysates for solid tumors.

Takashi Kamigaki, Masashi Takahara, Tsuyoshi Teruya

Recently, immunotherapy has been utilized as a novel option for the treatment of various malignancies, however, it is necessary to develop immune cell therapy on the basis of the blockade of immune checkpoints, antigen presentation on dendritic cell (DC) vaccines and the …

Precision Cancer Immunotherapy

Takuya Osada, Koji Nagaoka, Masashi Takahara, Xiao Yi Yang et autres

Most dendritic cell (DC)-based vaccines have loaded the DC with defined antigens, but loading with autologos tumor-derived antigens would generate DCs that activate personalized tumor-specific T-cell responses. We hypothesized that DC matured with an optimized combination of reagents and loaded with tumor-derived …

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Abstract 2539: Clinical prospective study of PepTivator® WT1-pulsed DC vaccines with assessment of immunological responses in solid tumors

Shoko Saiwaki, Shigenori Goto, Masashi Takahara, Haruka Matsushita et autres

Abstract Wilms' tumor gene 1 (WT1) is reported to be expressed in various types of malignancies and be one of promising target antigens for cancer immunotherapy. However, the synthetic short peptide vaccines based on the WT1 sequences limit a candidate patient by …

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Large-scale expansion of γδ T cells and peptide-specific cytotoxic T cells using zoledronate for adoptive immunotherapy

Toshiaki Yoshikawa, Masashi Takahara, Mai Tomiyama, Mie Nieda et autres

Specific cellular immunotherapy for cancer requires efficient generation and expansion of cytotoxic T lymphocytes (CTLs) that recognize tumor-associated antigens. However, it is difficult to isolate and expand functionally active T-cells ex vivo. In this study, we investigated the efficacy of a new …

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Induction of antigen specific T cells using PepTivator-pulsed dendritic cells

Masashi Takahara, Saori Goto, Kenji Miki, Shoko Saiwaki et autres

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IMMUNOTHERAPY/BIOLOGICAL THERAPIES

Jian Li Campian, Douglas Edward Gladstone, Prakash Ambady, X. Ye et autres

BACKGROUND: Patients with high grade gliomas (HGGs) routinely receive radiation (RT), temozolomide (TMZ), and glucocorticoids and 40% develop severe lymphopenia (lymphocyte counts ,500 cells/mm 3 ) 2-months after initiation of concurrent RT/TMZ (Grossman, 2011).The lymphopenia resulting from these three lymphotoxic therapies is …

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Zoledronate-pulsed dendritic cell-based anticancer vaccines

Takashi Kamigaki, Masashi Takahara, Ryuji Maekawa, Shigenori Goto

The addition of zoledronate to tumor-associated antigen (TAA)-loaded dendritic cells (DCs) promotes the activation of interferon γ-secreting Vγ9 γδ T cells, in turn eliciting TAA-specific CD8+ T-cell responses. Immunological responses induced by zoledronate-pulsed DC-based vaccines have been associated with therapeutic effects in …

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Immunotherapy of autologous tumor lysate-loaded dendritic cell vaccines by a closed-flow electroporation system for solid tumors.

Takashi Kamigaki, Toru Kaneko, Keiko Naitoh, Masashi Takahara et autres

Dendritic cell (DC)-based vaccines with the use of various antigen loading methods have been developed for cancer immunotherapy. Electroporation (EP) of a whole tumor cell lysate into DCs was previously found to be more potent for eliciting antigen-specific CD8 + T-cells compared …

Clinical scale electroloading of mature dendritic cells with melanoma whole tumor cell lysate is superior to conventional lysate co-incubation in triggering robust in vitro expansion of functional antigen-specific CTL

Lawrence A. Wolfraim, Masashi Takahara, Angelia M. Viley, Rama Shivakumar et autres

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Abstract 1562: Efficient antigen presentation and induction of anti-tumor immunity by dendritic cells loaded with antigens by electroporation

Koji Nagaoka, Masashi Takahara, Takayuki Yoshimoto, Tsuyoshi Teruya et autres

Abstract For successful dendritic cell (DC) therapy of cancer, it is important to choose the optimal method to load DCs with antigens to induce higher anti-tumor immunity, particularly tumor-specific CTL responses. The co-incubation (Co) method is conventionally used to load DCs with …

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Abstract 3508: Zoledronate induce expansion of glypican-3 (GPC3) peptide specific cytotoxic T lLymphocytes sufficient for adoptive cancer immunotherapy

Toshiaki Yoshikawa, Masashi Takahara, Mai Tomiyama, Ayumi Kuroda et autres

Abstract BACKGROUND Glypican-3 (GPC3) is an onco-fetal antigen which is overexpressed in human hepatocellular carcinoma (HCC), and is only expressed in the placenta and embryonic liver among normal tissues. Previously, we completed a phase I clinical trial of HLA-A2-restricted GPC3144-152 (FVGEFFTDV) and …

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