Accès ouvert
2024
article
OpenAlex
Tetsuro Tamaki, Toshiharu Natsume, Akira Katoh, Atsuko Shigenari et autres
Pig skeletal muscle-derived stem cells (SK-MSCs) were transplanted onto the common peroneal nerve with a collagen tube as a preclinical large animal experiment designed to address long nerve gaps. In terms of therapeutic usefulness, a human family case was simulated by adjusting …
jp
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Accès ouvert
2024
article
OpenAlex
Masayoshi Otake, Michihiro Imamura, Satoko Enya, Akihisa Kangawa et autres
Duchenne muscular dystrophy (DMD) is an intractable X-linked muscular dystrophy caused by mutations in the DMD gene. While many animal models have been used to study the disease, translating findings to humans has been challenging. Microminipigs, with their pronounced physiological similarity to …
jp
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Accès ouvert
2023
article
OpenAlex
Tatsuo Kawarasaki, Sinya Tutumi, Toshiaki Inenaga, Keisuke Matsukubo et autres
ゴム製手袋はブタ精子の性状に悪影響を及ぼすことが指摘されているが，現在もなおゴム製手袋がブタの精液採取時に使用されている。ゴム製品は，多くの成分から形成されており，製品によって精子に与える影響が異なる可能性がある。本研究では，現在流通しているゴム製品を含めた使い捨て手袋の材質が精子性状に与える影響について，わが国で養豚用に飼育されている一般品種を用いて検証した。実験1では，生ゴム，ニトリルゴム，加硫促進剤不使用のニトリルゴムおよびポリエチレン製手袋の断片と精液を共培養した。精子の運動性は，生ゴム，ニトリルゴムおよび加硫促進剤不使用のニトリルゴムでは著しく低下した。一方，ポリエチレンは共培養しないコントロールと差がなかった。先体の形態に与える害作用は運動性に比較すると軽度ではあったが，ニトリルゴムおよび加硫促進剤不使用のニトリルゴムでは，培養時間が長くなるにつれて増加した。実験2では，ニトリルゴムを水あるいは石鹸で十分に洗浄し，精液と共培養した。洗浄処理は精子の運動性や先体正常性に対する害作用を一定程度減少させたが，その効果は不十分なものであり，洗浄後も共培養した精子の運動性および先体正常性は共培養しないコントロールに比べて低い値を示した。実験3では，ポリエチレン製手袋4種類と精液を共培養した。1種類のポリエチレン製手袋において，運動性および先体正常性が低下したが，残り3種類はコントロールと差がなかった。これらの結果から，生ゴムおよび加硫促進剤不使用を含めたニトリル製ゴム製手袋は，ブタ精子の性状，特に運動性に対して著しい害作用を示すこと，この害作用は洗浄処理では十分に除去できないこと，ポリエチレン製手袋の多くは安全性が非常に高いことが明らかとなった。したがって，精液の採取や処理を行うときにはゴム製品の使用をやめ，ポリエチレン製品など安全性が高いものを使用することが推奨される。
jp
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Accès ouvert
2023
article
OpenAlex
Tetsuro Tamaki, Toshiharu Natsume, Akira Katoh, Nobuyuki Nakajima et autres
Large animal experiments are important for preclinical studies of regenerative stem cell transplantation therapy. Therefore, we investigated the differentiation capacity of pig skeletal muscle-derived stem cells (Sk-MSCs) as an intermediate model between mice and humans for nerve muscle regenerative therapy. Enzymatically extracted …
jp
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Accès ouvert
2023
preprint
OpenAlex
Tetsuro Tamaki, Toshiharu Natsume, Akira Kato, Nobuyuki Nakajima et autres
Large animal experiments are important for preclinical studies of regenerative stem cell transplantation therapy. Therefore, we investigated the differentiation capacity of pig skeletal muscle-derived stem cells (Sk-MSCs) as an intermediate model between mice and humans for nerve muscle regenerative therapy. Enzymatically extracted …
jp
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Tatsuo Kawarasaki, Satoko Enya, Masayoshi Otake
BACKGROUND/AIM: The microminipig is a relatively new type of mini pig; microminipigs weigh about 10 kg at 6 months of age and are expected to be of use in drug discovery research and safety tests. Herein, we analyzed the characteristics of ejaculated …
jp
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Accès ouvert
2021
article
OpenAlex
Masayoshi Otake, Hiroaki Kawaguchi, Satoko Enya, Akihisa Kangawa et autres
BACKGROUND/AIM: The reproducibility of athero - sclerotic lesions was evaluated after the production of cloned-microminipigs and their offspring. MATERIALS AND METHODS: Cloned-microminipig-parents were produced by microminipigsomatic cell nuclei. These parents were crossbred and delivered males (F1-offspring) were divided into two groups: normal …
jp
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Akihisa Kangawa, Toshiaki Nishimura, Takashi Nishimura, Masayoshi Otake et autres
Microminipigs have become an attractive animal model for toxicology and pharmacology studies and for human disease models, owing to their manageable size. Although there are numerous reports of spontaneous age-related lesions in mice, rats, dogs, and monkeys, those in minipigs are scarce. …
jp
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Accès ouvert
2019
article
OpenAlex
Akihisa Kangawa, Masayoshi Otake, Satoko Enya, Toshinori Yoshida et autres
Microminipigs have become an attractive animal model for the toxicology- and pharmacology-related studies because of their manageable size. In this study, the development of the testicular interstitium and steroidogenesis in microminipigs, from 0 to 12 months of age, were investigated. Testicular interstitium …
jp
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2017
article
OpenAlex
Tatsuo Kawarasaki, Satoko Enya, Masayoshi Otake, Masatoshi Shibata et autres
To assess the performance of boars derived by somatic cell cloning, we analyzed various aspects of their reproductive characteristics and the expression of two imprinted genes. Cloned boars (cloned Duroc × Jinhua) were analyzed for birth weight, growth rate, age at first …
jp
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Accès ouvert
2017
article
OpenAlex
Akihisa Kangawa, Masayoshi Otake, Satoko Enya, Toshinori Yoshida et autres
The microminipig has become an increasingly attractive animal model for various experimental practices because of its manageable size; however, studies of the histological features of the female reproductive organs in microminipigs are limited. The present study investigates the sexual development of the …
jp
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Accès ouvert
2017
article
OpenAlex
Satoko Enya, Tatsuo Kawarasaki, Masayoshi Otake, Akihisa Kangawa et autres
BACKGROUND/AIM: Microminipigs have been maintained in small populations of closed colonies, involving risks of inbreeding depression and genetic drift. In order to avoid these risks, we assessed the applicability of cloning technology. MATERIALS AND METHODS: Male and female clones were produced from …
jp
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