Ashlin R. Michell

Spatial patterning strategies for liver tissue engineering: Biofabrication technologies and applications

Haram Nah, Ashlin R. Michell, Kerry M Rogy, Owen J Lally et autres

The liver is composed of hepatocytes and non-parenchymal cells arranged in precise spatial patterns that enable more than 500 metabolic, synthetic, and detoxification functions. Replicating this hierarchical structure and dynamic multicellular organization is essential for applications in drug development and regenerative medicine. …

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Engineered cocultures of iPSC-derived atrial cardiomyocytes and atrial fibroblasts for modeling atrial fibrillation

Grace E. Brown, Yong Duk Han, Ashlin R. Michell, Olivia T Ly et autres

Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia treatable with antiarrhythmic drugs; however, patient responses remain highly variable. Human induced pluripotent stem cell-derived atrial cardiomyocytes (iPSC-aCMs) are useful for discovering precision therapeutics, but current platforms yield phenotypically immature cells and …

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Systems serology-based comparison of antibody effector functions induced by adjuvanted vaccines to guide vaccine design

Carolin Loos, Margherita Coccia, Arnaud M. Didierlaurent, Ahmed Essaghir et autres

Abstract The mechanisms by which antibodies confer protection vary across vaccines, ranging from simple neutralization to functions requiring innate immune recruitment via Fc-dependent mechanisms. The role of adjuvants in shaping the maturation of antibody-effector functions remains under investigated. Using systems serology, we …

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Delayed boosting improves human antigen-specific Ig and B cell responses to the RH5.1/AS01B malaria vaccine

Carolyn M. Nielsen, Jordan R. Barrett, Christine Davis, Jonathan K. Fallon et autres

Modifications to vaccine delivery that increase serum antibody longevity are of great interest for maximizing efficacy. We have previously shown that a delayed fractional (DFx) dosing schedule (0-1-6 month) - using AS01B-adjuvanted RH5.1 malaria antigen - substantially improves serum IgG durability as …

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Reanalysis of Trichloroethylene and Tetrachloroethylene Metabolism to Glutathione Conjugates Using Human, Rat, and Mouse Liver in Vitro Models to Improve Precision in Risk Characterization

Alan Valdiviezo, Grace E. Brown, Ashlin R. Michell, Cristiana M. Trinconi et autres

Background: Both trichloroethylene (TCE) and tetrachloroethylene (PCE) are high-priority chemicals subject to numerous human health risk evaluations by a range of agencies. Metabolism of TCE and PCE determines their ultimate toxicity; important uncertainties exist in quantitative characterization of metabolism to genotoxic moieties …

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Delayed Booster Dosing Modulates Human Antigen-Specific Ig and B Cell Responses to the RH5.1/AS01 B Malaria Vaccine

Carolyn M. Nielsen, Jordan R. Barrett, Christine L. Davis, Jon Fallon et autres

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Delayed Booster Dosing Improves Human Antigen-Specific IG and B Cell Responses to the RH5.1/AS01 B Malaria Vaccine

Carolyn M. Nielsen, Jordan R. Barrett, Christine L. Davis, Jon Fallon et autres

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Delayed fractional dosing with RTS,S/AS01 improves humoral immunity to malaria via a balance of polyfunctional NANP6- and Pf16-specific antibodies

Jishnu Das, Jonathan K. Fallon, Timothy C. Yu, Ashlin R. Michell et autres

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Comprehensive Data Integration Approach to Assess Immune Responses and Correlates of RTS,S/AS01-Mediated Protection From Malaria Infection in Controlled Human Malaria Infection Trials

William C. Young, Lindsay N. Carpp, Sidhartha Chaudhury, Jason A. Regules et autres

RTS,S/AS01 (GSK) is the world's first malaria vaccine. However, despite initial efficacy of almost 70% over the first 6 months of follow-up, efficacy waned over time. A deeper understanding of the immune features that contribute to RTS,S/AS01-mediated protection could be beneficial for …

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Reduced blood-stage malaria growth and immune correlates in humans following RH5 vaccination

Angela M. Minassian, Sarah E. Silk, Jordan R. Barrett, Carolyn M. Nielsen et autres

BACKGROUND: . METHODS: adjuvant. We assessed safety, immunogenicity, and efficacy against blood-stage CHMI. Trial registered at ClinicalTrials.gov, NCT02927145. FINDINGS: reduction of the parasite growth rate and also identify other antibody feature sets by systems serology, including the plasma anti-RH5 IgA1 response, that …

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Viral Load Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 in Hospitalized Individuals With Coronavirus Disease 2019

James Regan, James P. Flynn, Alexandra Rosenthal, Hannah Jordan et autres

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) kinetics remain understudied, including the impact of remdesivir. In hospitalized individuals, peak sputum viral load occurred in week 2 of symptoms, whereas viremia peaked within 1 week of symptom-onset, suggesting early systemic seeding of SARS-CoV-2. …

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Compromised SARS-CoV-2-specific placental antibody transfer

Caroline Atyeo, Krista M. Pullen, Evan A. Bordt, Stephanie Fischinger et autres

SARS-CoV-2 infection causes more severe disease in pregnant women compared to age-matched non-pregnant women. Whether maternal infection causes changes in the transfer of immunity to infants remains unclear. Maternal infections have previously been associated with compromised placental antibody transfer, but the mechanism …

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