Caitlin Doubleday

The Mycobacterium tuberculosis Ku C-terminus orchestrates LigD activity through domain-specific interactions

Dana J. Sowa, Jinfeng Huang, Anmol Marwaha, Caitlin Doubleday et autres

Abstract Bacterial non-homologous end joining (NHEJ) is a DNA double-strand break (DSB) repair pathway that relies on the Ku–LigD complex to alleviate genomic instability. The Mycobacterium tuberculosis Ku C-terminus has been highlighted for its role in LigD recruitment to DNA DSBs and …

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Fungal kinesin-8 motors dimerize by folding their proximal tail domain into a compact helical bundle

Daria Trofimova, Caitlin Doubleday, Byron Hunter, E. C. DAVISON et autres

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Kinesin-8 motors dimerize by folding their proximal tail domain into a compact helical bundle

Daria Trofimova, Caitlin Doubleday, Byron Hunter, Jesus Serrano Arevalo et autres

Abstract Kinesin-8 motor proteins help align and segregate chromosomes during mitosis by regulating the dynamics of kinetochore-attached microtubules and the length and position of the mitotic spindle. Some kinesin-8 isoforms accomplish these roles by operating as multifunctional mechanoenzymes that can traverse microtubules, …

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Myxococcus xanthus translesion DNA synthesis protein ImuA is an ATPase enhanced by DNA

Kristi Lichimo, Dana J. Sowa, Andriana Tetenych, Monica M. Warner et autres

Translesion DNA synthesis pathways are necessary to ensure bacterial replication in the presence of DNA damage. Translesion DNA synthesis carried out by the PolV mutasome is well-studied in Escherichia coli, but ~one third of bacteria use a functionally homologous protein complex, consisting …

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Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape

Byron Hunter, Matthieu P. M. H. Benoit, Ana B. Asenjo, Caitlin Doubleday et autres

Kinesin-8s are dual-activity motor proteins that can move processively on microtubules and depolymerize microtubule plus-ends, but their mechanism of combining these distinct activities remains unclear. We addressed this by obtaining cryo-EM structures (2.6-3.9 Å) of Candida albicans Kip3 in different catalytic states …

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Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape

Byron Hunter, Matthieu P. M. H. Benoit, Ana B. Asenjo, Caitlin Doubleday et autres

Kinesin-8s are dual-activity motor proteins that can move processively on microtubules and depolymerize microtubule plus-ends, but their mechanism of combining these distinct activities remains unclear. We addressed this by obtaining cryo-EM structures (2.6-3.9 Å) of Candida albicans Kip3 in different catalytic states …

ca, us (code pays fourni par la source)

Novel Immuno-assay for Detection of Lipophilic Substances v1

Ruben Warkentin, Caitlin Doubleday

Novel Immuno-assay for Detection of Lipophilic Substances Here we provide a methodology for testing antibodies for their binding ability to a lipophilic antigen. This approach was tested using a recombinantly expressed anti-THC-mNG antibody.