Magdalena Heberlein

Author response for "Studying the evolutionary potential of ancestral aryl sulfatases in the alkaline phosphatase family with droplet microfluidics"

Bernard D. G. Eenink, Josephin Marie Holstein, Magdalena Heberlein, Carina Dilkaute et autres

Studying the evolutionary potential of ancestral aryl sulfatases in the alkaline phosphatase family with droplet microfluidics

Bernard D. G. Eenink, Josephin Marie Holstein, Magdalena Heberlein, Carina Dilkaute et autres

enzymes with desired and effective functions. Most proteins organize in families, sets of divergent sequences which share a common ancestor and have a similar structural fold. Here, we study aryl sulfatases, a subfamily of the large and evolutionary old alkaline phosphatase superfamily. …

de, gb, pl (code pays fourni par la source)

Studying the evolutionary potential of ancestral aryl sulfatases in the alkaline phosphatase family with droplet microfluidics

Bernard D. G. Eenink, Josephin Marie Holstein, Magdalena Heberlein, Carina Dilkaute et autres

Abstract Characterizing the dynamics and functional shifts during protein evolution is essential, both for understanding protein evolution and for rationalizing efficient strategies for e.g. enzymes with desired and effective functions. Most proteins organize in families, sets of divergent sequences which share a …

de, gb, pl (code pays fourni par la source)

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

Bert van Loo, Magdalena Heberlein, Philip Mair, Anastasia Zinchenko et autres

Directed evolution of enzymes toward improved catalytic performance has become a powerful tool in protein engineering. To be effective, a directed evolution campaign requires the use of high-throughput screening. In this study we describe the development of an ultra high-throughput lysis-free procedure …

de, gb (code pays fourni par la source)

High-Throughput, Lysis-free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

Bert van Loo, Magdalena Heberlein, Philip Mair, Anastasia Zinchenko et autres

Abstract Directed evolution of enzymes toward improved catalytic performance has become a powerful tool in protein engineering. To be effective, a directed evolution campaign requires the use of high-throughput screening. In this study we describe the development of a high-throughput lysis-free procedure …

de, gb (code pays fourni par la source)

Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester

Bert van Loo, Markus Schöber, Eugene Valkov, Magdalena Heberlein et autres

Hydrolysis of organic sulfate esters proceeds by two distinct mechanisms, water attacking at either sulfur (S–O bond cleavage) or carbon (C–O bond cleavage). In primary and secondary alkyl sulfates, attack at carbon is favored, whereas in aromatic sulfates and sulfated sugars, attack …

de, gb, at (code pays fourni par la source)

Functional transitions in enzyme evolution: Balancing stability, folding and catalytic specificity

Bert van Loo, Magdalena Heberlein, Elias Dohmen, Andreas Lange et autres

Faculty Opinions recommendation of Ancestral Protein Reconstruction Yields Insights into Adaptive Evolution of Binding Specificity in Solute-Binding Proteins.

Erich Bornberg‐Bauer, Magdalena Heberlein

de (code pays fourni par la source)

Faculty Opinions recommendation of Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.

Erich Bornberg‐Bauer, Magdalena Heberlein

de (code pays fourni par la source)

Faculty Opinions recommendation of Life cycles, fitness decoupling and the evolution of multicellularity.

Erich Bornberg‐Bauer, Magdalena Heberlein

de (code pays fourni par la source)

Faculty Opinions recommendation of Intermolecular epistasis shaped the function and evolution of an ancient transcription factor and its DNA binding sites.

Erich Bornberg‐Bauer, Magdalena Heberlein

de (code pays fourni par la source)

Faculty Opinions recommendation of Assessing the prediction fidelity of ancestral reconstruction by a library approach.

Erich Bornberg‐Bauer, Magdalena Heberlein

de (code pays fourni par la source)