Yanan Li

Renal clearable CRISPR nanosensor targeting mitochondrial DNA mutation for noninvasive monitoring of tumor progression and metastasis

Yanan Li, Yonghua Wu, Yige Wu, Yongyuan Zhang et autres

Mitochondrial DNA (mtDNA) mutations are emerging as important molecular features of tumorigenesis. Liquid biopsies, involving analysis of cell-free mtDNA, enable early cancer detection but suffer from low sensitivity due to scarce analytes. Here, we developed a CRISPR/Cas12a-mediated urinary biomarker, termed CasUber, for …

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Divergent patterns of global tuna fishing fleet dynamics among different continents

Xin Cheng, Bo Zhang, Yanan Li, Hongyu Lin et autres

The sustainability of tuna fisheries relies on effective management measures and understanding the various regional patterns of fleet dynamics is helpful to inform the international collaborations of fisheries management at the global scale. In this study, we proposed a novel method based …

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Current status and influencing factors of clinical teachers’ teaching abilities for Master of Nursing Specialist postgraduates: a cross-sectional study †

Yanan Li, Jing Jing Wang, Shi Yan, Nana Liu et autres

Abstract Objective To assess the teaching efficacy of clinical instructors for MNS postgraduates, this study aims to gather insights into clinical educators’ perceptions concerning MNS postgraduate training and identify key competencies required for effective teaching. Methods This study utilized a cross-sectional design. …

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Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolution

Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu et autres

Background The widespread and evolution of RNA viruses, such as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the importance of fast identification of virus subtypes, particularly in non-laboratory settings. Rapid and inexpensive at-home testing of viral nucleic acids with single-base …

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Integration of CRISPR/Cas12a and Cas13a in one pot for ratiometric calibration of single-nucleotide variations

Hua Gao, Yongyuan Zhang, Yuan-Ming Wang, Yige Wu et autres

Accurate detection of single-nucleotide variations (SNVs) plays a pivotal role in medical diagnosis. Herein, by integrating Cas12a and Cas13a to simultaneously detect wild-type and mutated sites on a single RNA strand, we developed an innovative one-pot SNV analysis method, namely iCasdrop, which …

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Exploring the Influence of Mobile Phone Usage on the Cognitive Development of Rural Children

Yanan Li

Adrenal Surgery: Case 2 Laparoscopic Retroperitoneal Right Adrenalectomy

Yanan Li

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In vivo CRISPR biosensing

Yanan Li, Wen He Zhao, Yonghua Wu, Rui Li et autres

Herein, we systematically elaborate on the foundational principles, design methodologies, and diverse applications of in vivo CRISPR biosensing.

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Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform

Yanan Li, Fenglei Quan, Yige Wu, Yongyuan Zhang et autres

Quantitative analysis of cell-free RNA (cfRNA) in plasma sample can be used for screening, diagnosing, and prognosticating of multiple diseases. Here, we report a quantitative CRISPR/Cas digital imaging platform (qCasdip) for the detection of various cfRNAs, including circular RNAs and miRNAs, in …

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Retrospective study of safety of early enteral nutrition in severe intra-abdominal infection patients with intestinal fistula in a tertiary referral centre

Yanan Li, Tian Xie, Peng Wang

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Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes

Hongyan Wang, Jian-Yang Chen, Yanan Li, Xianghui Zhang et autres

Elucidating cellular architecture and cell-type evolution across species is central to understanding immune system function and susceptibility to disease. Adaptive immunity is a shared trait of the common ancestor of cartilaginous and bony fishes. However, evolutionary features of lymphocytes in these two …

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The predictive value of PFKFB3 in bladder cancer prognosis

Dongxu Qiu, Bin Zhao, Wenda Wang, Guoyang Zheng et autres

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-3 (PFKFB3) influences cancer progression via participating in tumor aerobic glycolysis. In this study, we aimed to evaluate the prognostic significance of PFKFB3 in bladder cancer (BLCA) patients by analyzing a combination of publicly available databases, clinical patient data, and bladder tumor …

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