Magdalena Zakrzewska

Serum microRNA qPCR profiling and validation indicate upregulation of circulating miR-145-5p and miR-26a-5p in migraineurs

Joanna Kordacka, Renata Gruszka, Magdalena Zakrzewska

BACKGROUND: In recent years, miRNAs found in biological fluids have gained interest as biomarkers of numerous conditions, including migraine. This study aimed to identify differences in the levels of circulating miRNAs in the serum of migraineurs as compared to healthy controls, as …

pl (code pays fourni par la source)

A 13-Year-Old Girl Affected by Melanocytic Tumors of the Central Nervous System—The Case

Emilia Nowosławska, Magdalena Zakrzewska, Beata Sikorska, Jakub Zakrzewski et autres

Primary intracranial melanoma is a very rare brain tumor, especially when accompanied by benign intramedullary melanocytoma. Distinguishing between a primary central nervous system (CNS) lesion and metastatic melanoma is extremely difficult, especially when the primary cutaneous lesion is not visible. Here we …

pl (code pays fourni par la source)

Identification and validation of miRNA-target genes network in pediatric brain tumors

Renata Gruszka, Jakub Zakrzewski, Emilia Nowosławska, Wiesława Grajkowska et autres

Alterations in miRNA levels have been observed in various types of cancer, impacting numerous cellular processes and increasing their potential usefulness in combination therapies also in brain tumors. Recent advances in understanding the genetics and epigenetics of brain tumours point to new …

pl (code pays fourni par la source)

Autophagy Inhibition with Chloroquine Increased Pro-Apoptotic Potential of New Aziridine-Hydrazide Hydrazone Derivatives against Glioblastoma Cells

Monika Witusik‐Perkowska, Pola Głowacka, Adam Marek Pieczonka, Ewa Świderska et autres

Tumor therapy escape due to undesired side effects induced by treatment, such as prosurvival autophagy or cellular senescence, is one of the key mechanisms of resistance that eventually leads to tumor dormancy and recurrence. Glioblastoma is the most frequent and practically incurable …

pl (code pays fourni par la source)

mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors

Renata Gruszka, Krzysztof Marcin Zakrzewski, Pawel Piotr Liberski, Magdalena Zakrzewska

Numerous molecular factors disrupt the correctness of the cell cycle process leading to the development of cancer due to increased cell proliferation. Among known causative factors of such process is abnormal gene expression. Nowadays in the light of current knowledge such alterations …

pl (code pays fourni par la source)

microRNA interaction with MAPK and AKT pathways in paediatric brain tumours – preliminary results and review of the literature

Renata Gruszka, Krzysztof Marcin Zakrzewski, Pawel Piotr Liberski, Magdalena Zakrzewska

Nowadays molecular investigations have a significant impact on the understanding of primary brain tumour biology,as well as on their classification and progress in the treatment modalities. Among novel type of biomarkers with potential therapeutic value, microRNAs (miRNAs) are considered in some cases. …

pl (code pays fourni par la source)

Sensitive detection of FGFR1 N546K mosaic mutation in patient with encephalocraniocutaneous lipomatosis and pilocytic astrocytoma

Joanna Kordacka, Krzysztof Marcin Zakrzewski, Renata Gruszka, Monika Witusik‐Perkowska et autres

Encephalocraniocutaneous lipomatosis (ECCL) is a rare neurocutaneous disorder, with only about 100 cases reported worldwide. It is characterized by congenital lesions of the eye, skin, and central nervous system. Only recently, potential causative FGFR1 point mutations have been identified in brain tumors …

pl (code pays fourni par la source)

Expression-based decision tree model reveals distinct microRNA expression pattern in pediatric neuronal and mixed neuronal-glial tumors

Magdalena Zakrzewska, Renata Gruszka, Konrad Stawiski, Wojciech M Fendler et autres

BACKGROUND: The understanding of the molecular biology of pediatric neuronal and mixed neuronal-glial brain tumors is still insufficient due to low frequency and heterogeneity of those lesions which comprise several subtypes presenting neuronal and/or neuronal-glial differentiation. Important is that the most frequent …

pl, us (code pays fourni par la source)

Artificial microenvironment of in vitro glioblastoma cell cultures changes profile of miRNAs related to tumor drug resistance

Monika Witusik‐Perkowska, Magdalena Zakrzewska, Dariusz Jan Jaskólski, Pawel Piotr Liberski et autres

Purpose: The in vitro environment can influence not only the molecular background of glioblastoma drug-resistance and treatment efficiency, but also the mechanisms and pathways of cell death. Both crucial molecular pathways and the deregulation of miRNAs are thought to participate in tumor …

pl (code pays fourni par la source)

Additional file 9: of Expression-based decision tree model reveals distinct microRNA expression pattern in pediatric neuronal and mixed neuronal-glial tumors

Magdalena Zakrzewska, Renata Gruszka, Konrad Stawiski, Wojciech M Fendler et autres

Table S9. Genes targeted by selected 8 miRNAs along with the list of targeting miRNAs. Experimentally validated and predicted targets have been summarized in this table showing results of in silico analysis. (XLSX 164 kb)

Additional file 2: of Expression-based decision tree model reveals distinct microRNA expression pattern in pediatric neuronal and mixed neuronal-glial tumors

Magdalena Zakrzewska, Renata Gruszka, Konrad Stawiski, Wojciech M Fendler et autres

Table S2. Configuration of hyperparameters set a priori for modelling in WEKA software. (XLSX 9 kb)

Additional file 5: of Expression-based decision tree model reveals distinct microRNA expression pattern in pediatric neuronal and mixed neuronal-glial tumors

Magdalena Zakrzewska, Renata Gruszka, Konrad Stawiski, Wojciech M Fendler et autres

Table S5. List of miRNAs differentially expressed in BRAFV600E mutated and wild type cases (GG12, GG25, GG28, GG33, GG55). Negative fold change indicates downregulation in mutated cases. miRNAs selected for validation has been appended to the Table. FC fold change, FDR false …