John R. Bracht

Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures

Bianca Brown, Elizabeth Nagy, Ajuni K. Takkar, Naomi A. Greengold et autres

Organoids are three-dimensional structures that develop in cultures of stem cells, and resemble multicellular organs. While performing long-term culture of Taeniopygia guttata (zebra finch) germline tissue, we observed the formation of germline organoids from cultures of both sexes. These macro-scale structures contain …

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Gain and Loss of Heterozygosity in the Genome of the Asexual Nematode Halicephalobus mephisto

Ali Amini, John R. Bracht

Abstract Asexual reproduction often leads to loss of genetic diversity, but several mechanisms have evolved to maintain heterozygosity. The subterrestrial nematode, Halicephalobus mephisto, reproduces parthenogenetically, and here, we investigate how its genetic diversity $$-$$ - 1.15% SNP heterozygosity—is retained from generation to …

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TSGA10 as a Model of a Thermal Metabolic Regulator: Implications for Cancer Biology

Ali Amini, Farzad Taghizadeh‐Hesary, John R. Bracht, Babak Behnam

TSGA10, a multifunctional protein critical for mitochondrial coupling and metabolic regulation, plays a paradoxical role in cancer progression and carcinogenesis. Here, we outline a potential mechanism by which TSGA10 mediates metabolism in oncogenesis and thermal modulation. Initially identified in spermatogenesis, TSGA10 interacts …

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Evolution of a biological thermocouple by adaptation of cytochrome c oxidase in a subterrestrial metazoan, Halicephalobus mephisto

Megan N. Guerin, T. Ellis, Mark Joseph Ware, Alexandra Manning et autres

Abstract In this study, we report a biological temperature-sensing electrical regulator in the cytochrome c oxidase of the Devil Worm, Halicephalobus mephisto . This extremophile metazoan was isolated 1.3 km underground in a South African goldmine, where it adapted to heat and …

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Evolution of a biological thermocouple by adaptation of cytochrome c oxidase in a subterrestrial metazoan

Megan N. Guerin, T. Ellis, Mark Joseph Ware, Alexandra Manning et autres

Abstract In this study we report a naturally evolved temperature-sensing electrical regulator in the cytochrome c oxidase of the Devil Worm, Halicephalobus mephisto. This extremophile metazoan was isolated 1.3 km underground in a South African goldmine, where it adapted to heat and …

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Surface detection of SARS-CoV-2 by lateral flow LAMP

Isabelle Dahl Acker, Mark Joseph Ware, John R. Bracht

Abstract Slowing the transmission of SARS-CoV-2 requires rapid and accurate diagnostic testing. Toward this end, loop-mediated isothermal amplification (LAMP), an isothermal genomic detection method, offers great promise but the readout tends to be difficult because it does not generate linear DNA products. …

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Unrealized potential from smaller institutions: Four strategies for advancing STEM diversity

Monessha Jayabalan, Madeline Caballero, Alyssa D. Cordero, Brandyn M. White et autres

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Four strategies to help build a more inclusive STEM ecology

Monessha Jayabalan, Madeline Caballero, Alyssa Cordero, Brandyn White et autres

We provide implementation guidance for four strategies for diversifying science, technology, engineering, and mathematics (STEM). Note that each individual document was written by a subset of the authors listed here. See the author list of each document for contributions and correspondence.

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A Transcriptomic Pipeline Adapted for Genomic Sequence Discovery of Germline-Restricted Sequence in Zebra Finch,Taeniopygia guttata

Kathryn C. Asalone, Ajuni K Takkar, Colin J. Saldanha, John R. Bracht

Songbirds have an unusual genomic element which is only found in their germline cells, known as the germline-restricted chromosome (GRC). Because germ cells contain both GRC and non-GRC (or A-chromosome) sequences, confidently identifying the GRC-derived elements from genome assemblies has proven difficult. …

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Direct Detection of DNA and RNA on Carbon Fiber Microelectrodes Using Fast-Scan Cyclic Voltammetry

Thomas Asrat, Whirang Cho, Favian A. Liu, Sarah M. Shapiro et autres

High Resolution Image Download MS PowerPoint Slide DNA and RNA have been measured with many techniques but often with relatively long analysis times. In this study, we utilize fast-scan cyclic voltammetry (FSCV) for the subsecond codetection of adenine, guanine, and cytosine, first …

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Regional sequence expansion or collapse in heterozygous genome assemblies

Kathryn C. Asalone, Kara Ryan, Maryam Yamadi, Annastelle Cohen et autres

High levels of heterozygosity present a unique genome assembly challenge and can adversely impact downstream analyses, yet is common in sequencing datasets obtained from non-model organisms. Here we show that by re-assembling a heterozygous dataset with variant parameters and different assembly algorithms, …

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Epigenetic Regulation of Neuregulin-1 Tunes White Adipose Stem Cell Differentiation

Alyssa D. Cordero, Evan C. Callihan, Rana Said, Yasir Alowais et autres

Expansion of subcutaneous adipose tissue by differentiation of new adipocytes has been linked to improvements in metabolic health. However, an expandability limit has been observed wherein new adipocytes cannot be produced, the existing adipocytes become enlarged (hypertrophic) and lipids spill over into …

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