Kelly L. Harris

Fat depot-specific accumulation of benzo(a)pyrene metabolites in rats fed with western diet and its implications for colon cancer

Kelly L. Harris, Kenneth J. Harris, Markis’ Hamilton, Olugbemiga Ogunkua et autres

Obesity is one of the factors that potentiate the development of colorectal cancer (CRC). Our previous studies using a rat model, the Polyposis In the Rat Colon (PIRC) kindred type revealed that compared to regular diet (RD), the Western diet (WD) accelerated …

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Development of an error-corrected next-generation sequencing method for the quantification of hotspot cancer driver mutations

Kelly L. Harris

Low-frequency somatic mutations accumulate in normal tissues throughout life and contribute to cancer initiation, yet their detection is limited by the sensitivity of conventional sequencing methods. We describe CarcSeq, an error-corrected, targeted next-generation sequencing platform developed to quantify rare cancer driver mutations …

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Abstract C054: Pharmacogenomics evaluation of histone deacetylase inhibitors on the treatment of triple-negative breast cancer subtypes

Bright Obeng, Kelly L. Harris, Beverly Lyn‐Cook, Beverly Word

Abstract Triple-negative breast cancer (TNBC) is an aggressive breast cancer type with very few effective treatment options, prone to recurrence and metastasis, and has poor prognosis in affected patients. This cancer is characterized by a lack of estrogen, progesterone, and human epidermal …

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Tissue and Sex‐Specific Performance of a Cancer Driver Based Biomarker in rasH2 ‐Tg Mice

Kelly L. Harris, Jennifer Faske, Binsheng Gong, Barbara L. Parsons

The ability to predict rodent lifetime tumor responses from short-term exposures and a scientific basis for rodent to human extrapolation are unmet needs in cancer risk assessment. To address these needs, quantitation of cancer driver mutations (CDMs) was integrated with an error-corrected, …

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12543 Biochemical And Structural Studies Of Human 17-Hydroxysteroid Dehydrogenase Type 1 Mutations Engineered To Convert An NADPH-dependent Reductase To An NAD-dependent Oxidase

Nicole Motl, Kelly L. Harris, Dario Mizrachi, Richard J. Auchus et autres

Abstract Disclosure: N. Motl: None. K.L. Harris: None. D. Mizrachi: None. R.J. Auchus: None. E. Scott: None. Background: In intact cells, the hydroxysteroid dehydrogenases (HSDs) strongly favor either NADPH-dependent ketosteroid reduction or NAD+-dependent hydroxysteroid oxidation. For ketosteroid reductases, mutation of the arginine …

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Integrating direct air capture with algal biofuel production to reduce cost, energy, and GHG emissions

Shavonn D'Souza, John W. Johnston, Valerie M. Thomas, Kelly L. Harris et autres

We investigate the potential to reduce costs and greenhouse gas emissions of the utilization of direct air capture of CO2 (DAC) for the production of algal biofuel. We examine four integrated designs for a DAC system comprised of solid amine monolith adsorbents …

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Repeat treatment of organotypic airway cultures with ethyl methanesulfonate causes accumulation of somatic cell mutations without expansion of bronchial-carcinoma-specific cancer driver mutations

Yiying Wang, Yuan Le, Kelly L. Harris, Ying Chen et autres

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Value of a multidisciplinary geriatric oncology committee on patient care in a community-based, academic cancer center.

Gurbani Singh, Lena Morant, McKenzie Bedra, Jennifer Emel et autres

e13771 Background: The heterogeneity in health and functional ability among older patients makes the management of cancer a unique challenge. The Geriatric Oncology Program at the University of Maryland Baltimore Washington Medical Center (BWMC) was created to optimize cancer management and recommendations …

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Abstract 2440: Cancer driver mutations as quantitative biomarkers of cancer risk interspecies analyses using CarcSeq

Kelly L. Harris, Binsheng Gong, Barbara L. Parsons, Jennifer Faske et autres

Abstract Unmet needs in cancer risk assessment include the ability to predict rodent life-time tumor responses from short-term exposures and a scientific basis for rodent to human extrapolation. As an approach to address these needs, the use of hotspot cancer driver mutations …

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Acceleration of benzo(a)pyrene-induced colon carcinogenesis by Western diet in a rat model of colon cancer

Kelly L. Harris, Kenneth J. Harris, Leah D. Banks, Samuel E. Adunyah et autres

Colorectal cancer (CRC) is the third leading cause of cancer-related mortalities in the USA and around 52,550 people were expected to die from this disease by December 2023. The objective of this study was to investigate the effect of diet type on …

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Abstract 3511: Varying Treatment outcomes of small molecule STING Agonist ADU-S100 in intratumoral and intravesical treatment regimens in syngeneic murine MB49 and in the N-methyl-N-nitrosourea (MNU) rat Model of urothelial carcinoma

Alok Kumar Singh, Kara A. Lombardo, Monali Praharaj, James Liu et autres

Abstract Introduction: Intratumoral (IT) delivery of STING agonist ADU-S100 shows strong CD8+T cells-mediated antitumor immunity. However, rapid absorption from IT sites, short terminal half-life and adverse outcomes caused withdrawal of ADU-S100 from clinical trials. We reported development of BCG-STING, a preclinical candidate …

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Assessment of Clonal Expansion Using CarcSeq Measurement of Lung Cancer Driver Mutations and Correlation With Mouse Strain- and Sex-Related Incidence of Spontaneous Lung Neoplasia

Kelly L. Harris, Karen L. McKim, Meagan B. Myers, Binsheng Gong et autres

Quantification of variation in levels of spontaneously occurring cancer driver mutations (CDMs) was developed to assess clonal expansion and predict future risk of neoplasm development. Specifically, an error-corrected next-generation sequencing method, CarcSeq, and a mouse CarcSeq panel (analogous to human and rat …

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