Adrian F. Pegoraro

Collective synchrony in confluent, pulsatile epithelia

Wenhui Tang, Mehrana R. Nejad, Adrian F. Pegoraro, L. Mahadevan et autres

Collective cell migration lies at the intersection of developmental biology and non-equilibrium physics, where active processes give rise to emergent patterns that are biologically relevant. Here, we investigate dilatational modes--cycles of expansion and contraction--in epithelial monolayers, and show that the divergence of …

Evaluation of size and mass characterization methods of candidate environmentally relevant nanoscale plastic reference materials

Adrian F. Pegoraro, Maohui Chen, Zygmunt J. Jakubek, Isaac Montesdeoca Hof et autres

Abstract Micro- and nanoplastics are environmental contaminants of increasing concern due to their persistence, ubiquity, and potential impacts on ecosystems and human health. Validating emerging detection technologies for these substances and enabling interlaboratory comparability requires the development of appropriate reference materials (RMs). …

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Autoencoder-Based Detection of Nanoplastics in Biological Matrices via Infrared Hyperspectral Imaging

Daniel Prezgot, Sadman Sakib, Maohui Chen, Adrian F. Pegoraro et autres

Abstract Mid-infrared hyperspectral imaging is an emerging tool for the qualitative and quantitative analysis of micro- and nanoplastics (MNPs) and for the characterization of biological tissues and complex matrices. Detecting MNPs in biological materials is of particular interest for assessing toxicological and …

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Simple analytical models of spectral focusing stimulated Raman scattering microscopy

Alexander N. Harper, Albert Stolow, Adrian F. Pegoraro

Stimulated Raman Scattering (SRS) microscopy is a powerful, rapid label-free, and chemical-specific imaging modality. While the underlying physics is well understood and modeled numerically, we show that, with simple approximations, one can derive equations which enable rapid simulations. Here, we develop an …

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Applications of stimulated Raman scattering microscopy to mineralogy: Real-time quantification of spodumene in lithium-bearing ores

Alexander N. Harper, Jonathan Boisvert, Albert Stolow, Adrian F. Pegoraro et autres

Lithium is a critical component in rechargeable battery chemistries, leading to international interest in efficient ore characterization methods. Current industry-standard automated mineralogy systems (EDX) provide high-resolution mapping but are challenged by long analysis times and intensive sample preparation. Here we demonstrate the …

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Ferroptosis and Wnt/β-Catenin Signaling Triggered by Environmentally Relevant Nanoscale Polypropylene Plastics in Human Intestinal Models

Sadman Sakib, Maohui Chen, Stephanie Skerrett, Daniel Prezgot et autres

High Resolution Image Download MS PowerPoint Slide Nanoplastics generated through environmental weathering may disrupt epithelial barrier integrity by promoting oxidative damage and inflammation, yet most studies rely solely on pristine synthetic particles that lack surface chemistries representative of real-world aged plastics. Here, …

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Enhancing stimulated Raman spectroscopy using Kerr squeezing

Nikolay Kalinin, Kilian Scheffter, Hannah Gallop, Hanieh Fattahi et autres

In many state-of-the-art stimulated Raman spectroscopy (SRS) experiments, shot noise is the dominating noise source. We apply polarization-squeezed light generated in a silica fiber via the Kerr effect for SRS. The non-classical state of light allows for lowering the noise power below …

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Nanoplastic reference materials for biological and methodological assessment

Adrian F. Pegoraro, Maohui Chen, Sadman Sakib, Zygmunt J. Jakubek et autres

Abstract Plastic particles are pervasive across all environments, yet quantifying their total abundance remains challenging—especially for nanoplastics, which are problematic to separate and detect efficiently. This challenge is further exacerbated by the heterogeneous nature of environmental nanoplastics, making it difficult to determine …

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Lock-in amplitude-phase correlations for enhanced imaging and segmentation in stimulated Raman scattering microscopy

Alexander N. Harper, Jonathan Boisvert, Tassos Grammatikopoulos, Albert Stolow et autres

Modulation transfer-based signal recovery methods, such as stimulated Raman scattering (SRS) microscopy, commonly use lock-in amplifier amplitude and phase outputs, with each providing readily accessible yet complementary information. Here we show that 2D amplitude-phase correlations enhance both imaging and segmentation (chemical specificity) …

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Topology and Nuclear Size Determine Cell Packing on Growing Lung Spheroids

Wenhui Tang, Jessie Huang, Adrian F. Pegoraro, James H. Zhang et autres

Within multicellular living systems, cells coordinate their positions with spatiotemporal accuracy to form various tissue structures and control development. These arrangements can be regulated by tissue geometry, biochemical cues, as well as mechanical perturbations. However, how cells pack during dynamic three-dimensional multicellular …

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Chirp modulation stimulated Raman scattering microscopy

Adrian F. Pegoraro, Albert Stolow

Coherent Raman Microscopy (CRM), a nonlinear optical version of Raman microscopy, offers rapid, chemical-specific, label-free imaging. Unfortunately, competing background optical processes limit its sensitivity and contrast in many materials. Existing modulation transfer schemes developed to reduce these are based on well-known concepts …

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Lock-in amplitude-phase correlations in coherent Raman microscopy: application to lithium ores

Alexander N. Harper, Jonathan Boisvert, Siddarth Shivkumar, Leah Frackleton et autres

In Stimulated Raman Scattering (SRS) Microscopy, which is typically based on modulation transfer techniques, the Lock-In amplitude and phase provide easily measured, complementary information. This information characterizes both the strength of a signal and the lifetime of the underlying process on a …