Thomas Wilton

Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Environmental Samples v2

Erika Bujáki, Ben Bellekom, Alison Tedcastle, Jasmaine Lee et autres

This protocol covers amplification of poliovirus nucleic acid from concentrated sewage samples following enrichment by sewage concentration and RNA purification with the subsequent sequencing of barcoded VP1 amplicons. An overview of the full procedure if shown in Figure 1. The protocol was …

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Multi-viral target Direct Detection and Nanopore Sequencing (DDNS) v1

Alison B. Tedcastle, Erika Bujáki, Thomas Wilton, Javier Martin

This protocol describes the target specific amplification for direct detection of multiple viral pathogens from wastewater extracted RNA. This enables sensitive identification of multiple different viral pathogens of interest for both surveillance and research purposes. Targets include Enterovirus D68, Enterovirus 71, Rotavirus, …

Detection of circulating vaccine-derived poliovirus type 2 (cVDPV2) in wastewater samples: a wake-up call, Finland, Germany, Poland, Spain, the United Kingdom, 2024

Sindy Böttcher, Julian Kreibich, Thomas Wilton, Vanessa Saliba et autres

In 2024, circulating vaccine-derived poliovirus type 2 (cVDPV2) was detected in wastewater samples in Finland, Germany, Poland, Spain and the United Kingdom (UK). All strains were genetically linked, but sequence analysis showed high genetic diversity among the strains identified within individual wastewater …

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Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Environmental Samples v1

Erika Bujáki, Alison Tedcastle, Thomas Wilton, khurshida not provided et autres

This protocol covers amplification of poliovirus nucleic acid from concentrated sewage samples and the subsequent sequencing of barcoded VP1 amplicons. The protocol was successfully tested on concentrates prepared with two-phase separation method, spin-column concentration and bag-mediated filtration system. Poliovirus molecular detection sensitivity …

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Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v1

Erika Bujáki, Thomas Wilton, Dimitra Klapsa, Alison B. Tedcastle et autres

This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. To perform automated extraction, follow steps in Workflow A, and for manual extractions follow Workflow B diverging after …

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Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v2

Erika Bujáki, Thomas Wilton, Dimitra Klapsa, Alison B. Tedcastle et autres

This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. Start by preparing the reagents required for the number of samples extracted, including the controls. To perform manual …

gb (code pays fourni par la source)

Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v2

Erika Bujáki, Thomas Wilton, Dimitra Klapsa, Alison B. Tedcastle et autres

This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. Start by preparing the reagents required for the number of samples extracted, including the controls. To perform manual …

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Asymptomatic immunodeficiency-associated vaccine-derived poliovirus infections in two UK children

Anika Singanayagam, Dimitra Klapsa, Shirelle Burton-Fanning, Julian F. Hand et autres

Increasing detections of vaccine-derived poliovirus (VDPV) globally, including in countries previously declared polio free, is a public health emergency of international concern. Individuals with primary immunodeficiency (PID) can excrete polioviruses for prolonged periods, which could act as a source of cryptic transmission …

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Case report: Clearance of longstanding, immune-deficiency-associated, vaccine-derived polio virus infection following remdesivir therapy for chronic SARS-CoV-2 infection

William Hywel Bermingham, Benjamin Canning, Thomas Wilton, Michael Kidd et autres

The global polio eradication campaign has had remarkable success in reducing wild-type poliovirus infection, largely built upon the live attenuated Sabin oral poliovirus vaccine. Whilst rare, vaccine poliovirus strains may cause infection and subsequently revert to a neurovirulent type, termed vaccine-derived poliovirus …

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Sustained detection of type 2 poliovirus in London sewage between February and July, 2022, by enhanced environmental surveillance

Dimitra Klapsa, Thomas Wilton, Andrew M. Zealand, Erika Bujáki et autres

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Detection of Enterovirus D68 in Wastewater Samples from the UK between July and November 2021

Alison B. Tedcastle, Thomas Wilton, Elaine Pegg, Dimitra Klapsa et autres

Infection with enterovirus D68 (EV-D68) has been linked with severe neurological disease such as acute flaccid myelitis (AFM) in recent years. However, active surveillance for EV-D68 is lacking, which makes full assessment of this association difficult. Although a high number of EV-D68 …

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Changing composition of SARS-CoV-2 lineages and rise of Delta variant in England

Swapnil Mishra, Sören Mindermann, Mrinank Sharma, Charles Whittaker et autres

BACKGROUND: Since its emergence in Autumn 2020, the SARS-CoV-2 Variant of Concern (VOC) B.1.1.7 (WHO label Alpha) rapidly became the dominant lineage across much of Europe. Simultaneously, several other VOCs were identified globally. Unlike B.1.1.7, some of these VOCs possess mutations thought …

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