Nicole Josleyn

The DNA-based Lassa vaccine INO-4500 confers durable protective efficacy in cynomolgus macaques against lethal Lassa fever

Viviane M. Andrade, Kathleen A. Cashman, Kyle Rosenke, Eric R. Wilkinson et autres

We have previously developed a DNA-based vaccine, INO-4500, encoding the Lassa lineage IV glycoprotein precursor. INO-4500, when delivered with electroporation, elicited humoral and cellular responses, and conferred 100% protection in cynomolgus non-human primates. Here, we expanded the characterization of INO-4500 assessing immunogenicity …

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Inactivated rabies-based Lassa fever virus vaccine candidate LASSARAB protects nonhuman primates from lethal disease

Gabrielle Scher, Catherine Yankowski, Drishya Kurup, Nicole Josleyn et autres

Lassa fever virus (LASV), a member of the Arenavirus family, is the etiological agent of Lassa fever, a severe hemorrhagic disease that causes considerable morbidity and mortality in the endemic areas of West Africa. LASV is a rodent-borne CDC Tier One biological …

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Characterization of an Anti-Ebola Virus Hyperimmune Globulin Derived From Convalescent Plasma

Jonathan Ciencewicki, Andrew S. Herbert, Nadia Storm, Nicole Josleyn et autres

BACKGROUND: Convalescent plasma has been used to treat many viral diseases including Ebola. The manufacture of a purified anti-Ebola virus (EBOV) intravenous immunoglobulin (IVIG) from pooled convalescent plasma is described in this paper. METHODS: An enzyme-linked immunosorbent assay (ELISA) targeting an EBOV …

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Acute Late-Stage Myocarditis in the Crab-Eating Macaque Model of Hemorrhagic Smallpox

Reed F. Johnson, Lauren Keith, Timothy K. Cooper, Srikanth Yellayi et autres

Hemorrhagic smallpox, caused by variola virus (VARV), was a rare but nearly 100% lethal human disease manifestation. Hemorrhagic smallpox is frequently characterized by secondary bacterial infection, coagulopathy, and myocardial and subendocardial hemorrhages. Previous experiments have demonstrated that intravenous (IV) cowpox virus (CPXV) …

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Convalescent plasma anti–SARS-CoV-2 spike protein ectodomain and receptor-binding domain IgG correlate with virus neutralization

Eric Salazar, Suresh V. Kuchipudi, Paul A. Christensen, Todd N. Eagar et autres

The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the urgent need for assays that detect protective levels of neutralizing antibodies. We studied the relationship among anti-spike ectodomain (anti-ECD), anti-receptor-binding domain (anti-RBD) IgG titers, and SARS-CoV-2 virus neutralization (VN) titers …

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Development of an antibody cocktail for treatment of Sudan virus infection

Andrew S. Herbert, Jeffery W. Froude, Ramon A. Ortiz, Ana I. Kuehne et autres

Antibody-based therapies are a promising treatment option for managing ebolavirus infections. Several Ebola virus (EBOV)-specific and, more recently, pan-ebolavirus antibody cocktails have been described. Here, we report the development and assessment of a Sudan virus (SUDV)-specific antibody cocktail. We produced a panel …

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Development Of A Flow Cytometry Assay To Study Monkeypox Virus Infection And Immune Response Regulation in Nonhuman Primates

Nicole Josleyn

Monkeypox virus (MPXV) is divided into two clades, Congo Basin and West African. Congo Basin MPXV infection is associated with severe symptoms and fatality of up to 10 percent in humans; whereas, West African MPXV infection is associated with less severe symptoms. …

An in vitro/ex vivo human lung infection model for studying the macrophage innate immune response to Ebola virus Kikwit

Nicole Josleyn, Ana I. Kuehne, Cecilia M. O’Brien, Jennifer M. Brannan et autres

Abstract Ebola virus causes a severe and often fatal hemorrhagic fever in people, for which no approved vaccines or therapeutics are available. Disease pathogenesis and countermeasure studies are typically performed using animal models; however, ex vivo models recapitulating human immune responses may …

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Cytokine and chemokine profiles in a human respiratory disease model of Ebola virus

Cecilia M. O’Brien, Jennifer M. Brannan, Ana I. Kuehne, Nicole Josleyn et autres

Abstract Category A agents, such as Ebola virus, pose a great risk to national security because of high rates of transmission and mortality and the gravity of impact to public health. The use of Ebola virus as a bioweapon is a significant …

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Evaluation of the Activity of Lamivudine and Zidovudine against Ebola Virus

Yu Cong, Julie Dyall, Brit J. Hart, Lisa Evans DeWald et autres

In the fall of 2014, an international news agency reported that patients suffering from Ebola virus disease (EVD) in Liberia were treated successfully with lamivudine, an antiviral drug used to treat human immunodeficiency virus-1 and hepatitis B virus infections. According to the …

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Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices

Steven Mazur, Michael R. Holbrook, Tracey Burdette, Nicole Josleyn et autres

Work in a biosafety level 4 (BSL-4) containment laboratory requires time and great attention to detail. The same work that is done in a BSL-2 laboratory with non-high-consequence pathogens will take significantly longer in a BSL-4 setting. This increased time requirement is …

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Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices

Steven Mazur, Michael R. Holbrook, Tracey Burdette, Nicole Josleyn et autres

Work in a biosafety level 4 (BSL-4) containment laboratory requires time and great attention to detail. The same work that is done in a BSL-2 laboratory with non-high-consequence pathogens will take significantly longer in a BSL-4 setting. This increased time requirement is …

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