Drew M. Hansen

A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli

Kenneth Rachwalski, Megan M. Tu, Sean J. Madden, Shawn French et autres

Advances in gene editing, in particular CRISPR interference (CRISPRi), have enabled depletion of essential cellular machinery to study the downstream effects on bacterial physiology. Here, we describe the construction of an ordered E. coli CRISPRi collection, designed to knock down the expression …

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Development of High Throughput Screening Approaches to Target TN1549 and F Plasmid Movement

Drew M. Hansen

The antimicrobial resistance (AMR) crisis, where new antibiotic discovery is not keeping pace with the emergence of resistant pathogens, is driven by mobile genetic elements (MGEs). MGEs can autonomously transfer between bacteria, along with AMR genes. The widespread use of antibiotics in …