Rita Vanbever

Formation of a pulmonary drug-depot by a double-ester treprostinil prodrug enables sustained lung-selective delivery

Giuditta Leone, Kouamé Kan Firmin Akoumia, Bernard Učakar, Hrag Esfahani et autres

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Microliter-scale in vitro aerosol-cell delivery of inhalable antisense oligonucleotide (aso) induces “Burst-like” Pharmacokinetics

Pramod Kumar, Ashesh Chakraborty, Elias Richter, Lin Yang et autres

Unlike traditional submerged cell cultures, nebulized drug delivery to air-liquid interface (ALI)-cultured lung cells provides a clinically relevant route for in vitro testing of inhalable drugs. Current nebulizer systems deliver non-physiologically large drug volumes. Here, we introduce the Cloud MAX technology, characterize …

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Enzymes-enhanced antibiotic therapy reduces biofilms to undetectable levels in an implant-associated infection model

Randy Buzisa Mbuku, Hervé Poilvache, Loïc Maigret, Rita Vanbever et autres

Implant-associated infections caused by biofilm-forming bacteria, such as Staphylococcus aureus , remain a major clinical challenge due to their high tolerance to conventional antibiotic therapies. We report a dual-targeted therapeutic strategy that combines a tri-enzymatic cocktail designed to degrade key components of …

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Synthesis and in vitro evaluation of polyethylene glycol-treprostinil conjugates for sustained local delivery in pulmonary arterial hypertension

Bernard Učakar, Frédéric Perros, Raphaël Frédérick, Rita Vanbever

Treprostinil (TRE) is a prostacyclin analogue approved for the treatment of pulmonary arterial hypertension (PAH). Despite its effectiveness, TRE has a short half-life, necessitating frequent or continuous administration to maintain therapeutic levels while minimizing adverse effects. To improve pharmacokinetics and pulmonary targeting …

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Targeting Staphylococcus aureus biofilm-related infections on implanted material with a novel dual-action thermosensitive hydrogel containing vancomycin and a tri-enzymatic cocktail: in vitro and in vivo studies

Randy Buzisa Mbuku, Hervé Poilvache, Loïc Maigret, Rita Vanbever et autres

Implant-associated infections remain a critical challenge due to the presence of biofilm-forming bacteria, which enhance tolerance to conventional treatments. This study investigates the efficacy of a tri-enzymatic cocktail (TEC; DNA/RNA endonuclease, endo-14-β-D-glucanase, β- N -acetylhexosaminidase) targeting biofilm matrix components combined with supratherapeutic …

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Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy

Xiao Liu, Bernard Učakar, Kévin Vanvarenberg, Étienne Marbaix et autres

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Enzymatic, structural, and biophysical characterization of a single‐domain antibody (VHH) selectively and tightly inhibiting neutrophil elastase and exhibiting favorable developability properties

Paola Redeghieri, Joël Moray, Frédéric Kerff, Sophie Gohy et autres

Abstract Human neutrophil elastase (hNE), a serine protease released by neutrophils during inflammation, plays a major role in the pathophysiology of several conditions especially in inflammatory lung diseases. Its inhibition constitutes, therefore, a promising therapeutic strategy to combat these diseases. In this …

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Impact of protein aggregation on the immunogenicity of a human monoclonal antibody following pulmonary administration in mice

Sohaib Mahri, Céline Cassiers, Sandra Gracin, Donatienne Tyteca et autres

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Repurposing DNase I and alginate lyase to degrade the biofilm matrix of dual-species biofilms of Staphylococcus aureus and Pseudomonas aeruginosa grown in artificial sputum medium: In-vitro assessment of their activity in combination with broad-spectrum antibiotics

Zhifen Wang, Rita Vanbever, Joseph H. Lorent, Jessica Solis et autres

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Nebulization of PEGylated recombinant human deoxyribonuclease I using vibrating membrane nebulizers: A technical feasibility study

Sohaib Mahri, Tobias Wilms, Paul Hagedorm, Marie‐Julie Guichard et autres

Recombinant human deoxyribonuclease I (rhDNase, Pulmozyme®) is the most frequently used mucolytic agent for the symptomatic treatment of cystic fibrosis (CF) lung disease. Conjugation of rhDNase to polyethylene glycol (PEG) has been shown to greatly prolong its residence time in the lungs …

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Lipoid Pneumonia Associated with Polyethylene Glycol Chronic Aspiration

Pierre Brancaleone, Quentin Delefortrie, Olivier Descamps, Birgit Weynand et autres

status: Published

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Impact of the PEG length and PEGylation site on the structural, thermodynamic, thermal, and proteolytic stability of mono‐PEGylated alpha‐1 antitrypsin

Xiao Liu, Kobenan Guy Wilfried Kouassi, Rita Vanbever, Mireille Dumoulin

Conjugation to polyethylene glycol (PEG) is a widely used approach to improve the therapeutic value of proteins essentially by prolonging their body residence time. PEGylation may however induce changes in the structure and/or the stability of proteins and thus on their function(s). …

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