Michaella J. Levy

Carbohydrate‐Directed Labeling Probes for UV‐Independent Chemoproteomic Profiling of Carbohydrate–Protein Interactions

Matthew Russolillo, Wariya Nirachonkul, Patrick Ross, Rahul M. Hedau et autres

ABSTRACT Weak and transient carbohydrate–protein interactions hinder the detection of carbohydrate‐binding proteins (CBPs) in native settings. Here, we introduce carbohydrate‐directed labeling probes (CDLPs), modular ligand‐directed covalent probes composed of a glycan ligand, an acyl‐transfer electrophile, and a distal azide handle. Glycan binding …

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Carbohydrate‐Directed Labeling Probes for UV‐Independent Chemoproteomic Profiling of Carbohydrate–Protein Interactions

Matthew Russolillo, Wariya Nirachonkul, Patrick Ross, Rahul M. Hedau et autres

ABSTRACT Weak and transient carbohydrate–protein interactions hinder the detection of carbohydrate‐binding proteins (CBPs) in native settings. Here, we introduce carbohydrate‐directed labeling probes (CDLPs), modular ligand‐directed covalent probes composed of a glycan ligand, an acyl‐transfer electrophile, and a distal azide handle. Glycan binding …

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Targeting N-Myc in neuroblastoma with selective Aurora kinase A degraders

Jian Tang, Ramkumar Moorthy, Laura Hirsch, Özlem Demir et autres

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The antiproliferative effect of FGF2 in K-Ras-driven tumor cells involves modulation of rRNA and the nucleolus

Francisca N. de Luna Vitorino, Michaella J. Levy, Rosangela A.M. Wailemann, Mariana Lopes et autres

The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has antiproliferative and tumor-suppressive functions in some cellular contexts. In this work, we investigated how the antiproliferative …

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Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility

Luke Olsen, Michaella J. Levy, Kyle Medley, Huzaifa Hassan et autres

Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting. Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis. One such example, the …

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Paraspeckles interact with SWI/SNF subunit ARID1B to regulate transcription and splicing

Divya Reddy, Saikat Bhattacharya, Michaella J. Levy, Ying Zhang et autres

Paraspeckles are subnuclear RNA-protein structures that are implicated in important processes including cellular stress response, differentiation, and cancer progression. However, it is unclear how paraspeckles impart their physiological effect at the molecular level. Through biochemical analyses, we show that paraspeckles interact with …

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Abstract 2135: Targeting N-Myc in neuroblastoma cells with selective Aurora kinase A degraders

Jian Tang, Ramkumar Moorthy, Özlem Demir, Zachary D. Baker et autres

Abstract Overexpression of the MYCN proto-oncogene is one of the strongest biomarkers for poor prognosis in neuroblastoma patients. N-Myc, encoded by MYCN, is a highly attractive yet “undruggable” target in neuroblastoma drug discovery. N-Myc is a short-lived protein in normal neuronal progenitor …

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Targeting N-Myc in Neuroblastoma with Selective Aurora Kinase A Degraders

Jian Tang, Ramkumar Moorthy, Özlem Demir, Zachary D. Baker et autres

Summary Paragraph MYCN amplification is the most frequent genetic driver in high-risk neuroblastoma (NB) and strongly associated with poor prognosis. 1,2 The N-Myc transcription factor, which is encoded by MYCN , is a mechanistically validated, yet challenging target for NB therapy development. …

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Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility

Luke Olsen, Michaella J. Levy, Kyle Medley, Huzaifa Hassan et autres

Abstract Physical inactivity – specifically the lack of moderate-to-vigorous activity – is a scourge to human health, promoting metabolic disease and muscle wasting. Interestingly, multiple ecological niches have relaxed investment into physical activity, providing unique evolutionary insight into adaptive physical inactivity. The …

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A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis

Diego J. Páez-Moscoso, David V. Ho, Lili Pan, Katie Hildebrand et autres

Telomerase reverse transcriptase (TERT) and the noncoding telomerase RNA (TR) subunit constitute the core of telomerase. Additional subunits are required for ribonucleoprotein complex assembly and in some cases remain stably associated with the active holoenzyme. Pof8, a member of the LARP7 protein …

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A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis

Diego J. Páez-Moscoso, David V. Ho, Lili Pan, Katie Hildebrand et autres

Abstract Telomerase reverse transcriptase (TERT) and the noncoding telomerase RNA (TR) subunit constitute the core of telomerase. Additional subunits are required for ribonucleoprotein complex assembly and in some cases remain stably associated with the active holoenzyme. Pof8, a member of the LARP7 …

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Cytidine acetylation yields a hypoinflammatory synthetic messenger RNA

Kellie D. Nance, Supuni Thalalla Gamage, Md Masud Alam, Acong Yang et autres

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