Giulia Gallerani

CellSearch-based isolation of circulating tumor cells from ascitic fluid enables molecular characterization of ovarian cancer

Francesca Gorini, Thais Maloberti, Emma Coschina, Francesco Mezzapesa et autres

Background High-grade serous tubo-ovarian cancer (HGSOC) frequently presents with malignant ascites, a biologically relevant compartment rich in tumor cells. While circulating tumor cells (CTCs) in peripheral blood (PB) are often scarce, ascitic fluid (AF) may represent a more accessible and abundant source …

it (code pays fourni par la source)

Circulating tumor cells as biomarkers in melanoma: techniques, challenges, and clinical applications

Elisabetta Broseghini, Giorgio Durante, Alessia Soru, Franca Bianchi et autres

Cutaneous melanoma is a malignant tumor and remains a clinical challenge due to unmet needs in risk stratification, real-time treatment monitoring, and early detection of progression or resistance. Early detection is crucial, as prognosis significantly worsens once metastasis occurs. Liquid biopsy has …

it (code pays fourni par la source)

Cancer of unknown primary genomic profiling from cell-free DNA provides insights into CUP biology and vulnerabilities

Roberta Roncarati, Beatrice Fontana, Ilaria Pace, Giorgio Durante et autres

Introduction Cancer of unknown primary (CUP) is a metastatic malignancy with no identifiable site of origin, accounting for 2–5% of cancer diagnoses. Its marked heterogeneity and the limited availability of tumor tissue pose major challenges to genomic profiling. Methods In this retrospective …

it (code pays fourni par la source)

Abstract 3345: Horizontal transfer of functional extrachromosomal DNA via extracellular vesicles in FGFR2-amplified cancer

Irene Salamon, Giulia Gallerani, Jens Luebeck, Gianluca Storci et autres

Abstract Cancer cells actively release extracellular vesicles (EVs) into the tumor microenvironment, where they interact with both malignant and non-malignant cells, activating signaling pathways and reshaping the microenvironment. In this study, we investigated EVs secreted by FGFR2-amplified cancers of unknown primary (CUPs), …

it, us (code pays fourni par la source)

Abstract P3-01-17: Transcriptomic and phenotypic profiling of circulating tumor cells from metastatic breast cancer patients

Michela Palleschi, Tania Rossi, Sara Bandini, Michele Zanoni et autres

Abstract Background: Metastatic breast cancer (MBC) is considered an incurable disease and understanding the molecular mechanisms at the basis of tumor progression has become mandatory. Circulating tumor cells (CTCs) constitute a population of rare cells that are shed in the bloodstream by …

Transcriptomic profiling of circulating tumor cells from metastatic breast cancer patients reveals new hints in their biological features and phenotypic heterogeneity

Tania Rossi, Sara Bandini, Michele Zanoni, Michela Cortesi et autres

The study of circulating tumor cells (CTCs) provides critical insights into the biological mechanisms underlying metastasis. This study aims to demonstrate the applicability of an integrated DEPArray-based phenotypic analysis combined with transcriptomic characterization to investigate the biology of CTCs isolated from 10 …

it, ua (code pays fourni par la source)

Abstract 6569: Extracellular vesicle delivery of functional extrachromosomal DNA in FGFR2-amplified cancer of unknown primary

Irene Salamon, Giulia Gallerani, Gianluca Storci, Beatrice Fontana et autres

Abstract The extracellular vesicle (EV) route is essential for cell-to-cell communication. Cancer cells release EVs in the extracellular space, where they can interact with cancer and non-cancer cells, activating specific signaling pathways, modulating tumor microenvironment remodeling and inducing gene expression alterations. We …

it (code pays fourni par la source)

Gastroesophageal circulating tumor cell crosstalk with peripheral immune system guides CTC survival and proliferation

Tania Rossi, Martina Valgiusti, Maurizio Puccetti, Giacomo Miserocchi et autres

Tumor dissemination is a key event in tumor progression. During this event, a main role is played by circulating tumor cells (CTCs), immune cells, and their interaction. How the immune system supports the survival and proliferation of CTCs is not fully elucidated. …

it (code pays fourni par la source)

Extracellular vesicles deliver functional extrachromosomal DNA in FGFR2-amplified cancer of unknown primary

Irene Salamon, Giulia Gallerani, Gianluca Storci, Beatrice Fontana et autres

it, us (code pays fourni par la source)

Vaccine-Based Immunotherapy for Oropharyngeal and Nasopharyngeal Cancers

Daria Maria Filippini, Elisabetta Broseghini, Carlotta Liberale, Giulia Gallerani et autres

Viral infections such as human papillomavirus (HPV) and Epstein-Barr virus (EBV) play a critical role in the onset of oropharyngeal (OPC) and nasopharyngeal cancer (NPC), respectively. Despite advancements in targeted therapies and immunotherapies, in the recurrent/metastatic setting, these tumors remain incurable diseases …

it (code pays fourni par la source)

Synergic activity of FGFR2 and MEK inhibitors in the treatment of FGFR2-amplified cancers of unknown primary

Andrea Cavazzoni, Irene Salamon, Claudia Fumarola, Giulia Gallerani et autres

Patients with cancer of unknown primary (CUP) carry the double burden of an aggressive disease and reduced access to therapies. Experimental models are pivotal for CUP biology investigation and drug testing. We derived two CUP cell lines (CUP#55 and #96) and corresponding …

it, us (code pays fourni par la source)

Abstract 6957: Extrachromosomal FGFR2 is delivered in extracellular vesicles in two models of cancer of unknown primary

Irene Salamon, Gianluca Storci, Beatrice Fontana, Salvatore Serravalle et autres

Abstract The extracellular vesicle (EV) route is essential for cell-to-cell communication. Cancer cells release EVs in the extracellular space, where they can interact with recipient cells inducing regulation of gene expression, activation of specific signaling pathways and tumor microenvironment remodeling. We investigated …

it (code pays fourni par la source)