Shinji Asano

Unconstrained deep learning-based sleep stage classification using cardiorespiratory and body movement activities in adults with suspected sleep apnea

Seiichi Morokuma, Toshinari Hayashi, Masatomo Kanegae, Yoshihiko Mizukami et autres

This study assessed the feasibility of unconstrained deep-learning-based sleep stage classification using cardiorespiratory and body movement activities derived from piezoelectric sensors installed under a bed mattress. Heart and respiratory rates and their respective variabilities, cardiorespiratory coupling index, and body movement were simultaneously …

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Ciliary Motility Decreased by a CO2/HCO3−-Free Solution in Ciliated Human Nasal Epithelial Cells Having a pH Elevated by Carbonic Anhydrase IV

Shota Okamoto, Makoto Yasuda, Kotoku Kawaguchi, Kasane Yasuoka et autres

An application of CO2/HCO3−-free solution (Zero-CO2) did not increase intracellular pH (pHi) in ciliated human nasal epithelial cells (c-hNECs), leading to no increase in frequency (CBF) or amplitude (CBA) of the ciliary beating. This study demonstrated that the pHi of c-hNECs expressing …

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The Increase in the Frequency and Amplitude of the Beating of Isolated Mouse Tracheal Cilia Reactivated by ATP and cAMP with Elevation in pH

Akari Kobayashi, Kotoku Kawaguchi, Shinji Asano, Hong Ying Wu et autres

Single cilia, 100 nm in diameter and 10 µm in length, were isolated from mouse tracheae with Triton X-100 (0.02%) treatment, and the effects of pH on ciliary beating were examined by measuring the ciliary beat frequency (CBF) and the ciliary bend …

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A decrease in ciliary motility induced by a CO2/HCO3--free solution characteristic of ciliated human nasal epithelial cells: acceleration of HCO3- transport by carbonic anhydrase IV

Makoto Yasuda, Kotoku Kawaguchi, Kasane Yasuoka, Yumi Kikukawa et autres

An application of CO2/HCO3--free solution (Zero-CO2) did not increase intracellular pH (pHi) in ciliated human nasal epithelial cells (c-hNECs), leading to no increase in the beating frequency (CBF) or bend distance (CBD, an index of amplitude). This study demonstrated that the pHi …

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Adenosine Stimulates Beating of Neonatal Brain-Derived Cilia through Adenosine A2B Receptor on the Cilia and Activation of Protein Kinase A Pathway

Kotoku Kawaguchi, Suzuka Tsuji, Takuya Hirao, Yixin Liu et autres

Motile cilia in the ependymal cells that line the brain ventricles play pivotal roles in cerebrospinal fluid (CSF) flow in well-defined directions. However, the substances and pathways which regulate their beating have not been well studied. Here, we used primary cultured cells …

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Inhibition of ezrin phosphorylation by NSC305787 attenuates procaterol-stimulated ciliary beating in airway cilia

Kotoku Kawaguchi, S YOKOYAMA, Haoting Wang, Shinji Asano

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Ambroxol-Enhanced Frequency and Amplitude of Beating Cilia Controlled by a Voltage-Gated Ca2+ Channel, Cav1.2, via pHi Increase and [Cl−]i Decrease in the Lung Airway Epithelial Cells of Mice

Takashi Nakahari, Chihiro Suzuki, Kotoku Kawaguchi, Shigekuni Hosogi et autres

Ambroxol (ABX), a frequently prescribed secretolytic agent which enhances the ciliary beat frequency (CBF) and ciliary bend angle (CBA, an index of amplitude) by 30%, activates a voltage-dependent Ca2+ channel (CaV1.2) and a small transient Ca2+ release in the ciliated lung airway …

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Deep learning-based sleep stage classification with cardiorespiratory and body movement activities in individuals with suspected sleep disorders

Seiichi Morokuma, Toshinari Hayashi, Masatomo Kanegae, Yoshihiko Mizukami et autres

Deep learning methods have gained significant attention in sleep science. This study aimed to assess the performance of a deep learning-based sleep stage classification model constructed using fewer physiological parameters derived from cardiorespiratory and body movement data. Overnight polysomnography (PSG) data from …

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Ambroxol-enhanced frequency and amplitude of beating cilia mediated by pHi and [Cl-]i in lung airway epithelial cells of mice

Takashi Nakahari, Chihiro Suzuki, Kotoku Kawaguchi, Shigekuni Hosogi et autres

Abstract Ambroxol (ABX), a frequently prescribed secretolytic agent that stimulates Ca2+ release from acidic stores and Ca2+ entry through CaV1.2, enhanced the ciliary beat frequency (CBF) and ciliary bend angle (CBA, an index of amplitude) by 30% in ciliated lung airway epithelial …

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Nasal ciliary beating controlled by carbonic anhydrase IV and Na+/HCO3- co-transport under an extremely low CO2 condition (air)

Shota Okamoto, Makoto Yasuda, Kotoku Kawaguchi, Kasane Yasuoka et autres

Abstract Ciliated human nasal epithelial cells (c-hNECs) express the bicarbonate transport metabolon (BTM) consisting of carbonic anhydrase (CA) IV, Na+/HCO3- cotransporter (NBC) and CAII. This study demonstrated that the BTM rapidly and selectively transports HCO3- into c-hNECs resulting in a high intracellular …

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Identification of TEKTIN1-expressing multiciliated cells during spontaneous differentiation of non-human primate embryonic stem cells

Tomomi Nishie, Yoshio Ohta, Emi Shirai, Shogo Higaki et autres

Tektins are a group of microtubule-stabilizing proteins necessary for cilia and flagella assembly. TEKTIN1 (TEKT1) is used as a sperm marker for monitoring germ cell differentiation in embryonic stem (ES) and induced pluripotent stem (iPS) cells. Although upregulation of TEKT1 has been …

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Identification of TEKTIN1‐expressing multiciliated cells during spontaneous differentiation of non‐human primate embryonic stem cells

Tomomi Nishie, Yoshio Ohta, Emi Shirai, Shogo Higaki et autres

Tektins are a group of microtubule-stabilizing proteins necessary for cilia and flagella assembly. TEKTIN1 (TEKT1) is used as a sperm marker for monitoring germ cell differentiation in embryonic stem (ES) and induced pluripotent stem (iPS) cells. Although upregulation of TEKT1 has been …

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