Johannes Elferich

Programmable gold nanoparticle conjugates enable precise AMPA receptor localization at brain synapses by cryo-ET

Cathy J. Spangler, Gunasekaran Dhandapani, Aya Matsui, Elizaveta V Shiltseva et autres

Cryo-electron tomography (cryo-ET) is a powerful approach to image cells, organelles, viruses and molecular assemblies. However, resolving smaller complexes and molecules is challenging and presents a roadblock to the broad application of cryo-ET. Gold nanoparticles (AuNPs) have been used to localize otherwise …

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Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model

Lingli Kong, Ximena Zottig, Johannes Elferich, Nikolaus Grigorieff

Abstract The exposure of frozen biological samples to the high-energy electron beam in a cryo-electron microscope commonly leads to beam-induced sample motion and distortions. Previously, we described Unblur , which is part of our cis TEM software to correct for beam-induced motion …

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Author response: Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model

Lingli Kong, Ximena Zottig, Johannes Elferich, Nikolaus Grigorieff

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teamtomo/torch-grid-utils: v0.1.2

Alister Burt, Marten Chaillet, Josh Dickerson, aysecanuenal et autres

What's Changed ci(dependabot): bump codecov/codecov-action from 5 to 6 by @dependabot[bot] in https://github.com/teamtomo/torch-grid-utils/pull/27 Full Changelog: https://github.com/teamtomo/torch-grid-utils/compare/v0.1.1...v0.1.2

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Author Response: Unbend: Correction of local beam-induced sample motion in cryo-EM images using a 3D spline model

Lingli Kong, Ximena Zottig, Johannes Elferich, Nikolaus Grigorieff

The exposure of frozen biological samples to the high-energy electron beam in a cryo-electron microscope commonly leads to beam-induced sample motion and distortions. Previously, we described Unblur, which is part of our cisTEM software to correct for beam-induced motion based on the …

The protocadherin-15-LHFPL5 tip link complex is a heterotetrameric assembly in hair cell stereocilia

Sarah Clark, Jaba Mitra, Johannes Elferich, April S. Goehring et autres

Hearing and balance rely on the conversion of a mechanical stimulus into an electrical signal, a process known as mechanosensory transduction (MT). In vertebrates, this process is accomplished by a MT complex located in hair cells of the inner ear. While the …

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teamtomo/torch-grid-utils: v0.0.10

A. Yu. Burt, Marten Chaillet, aysecanuenal, Josh Dickerson et autres

What's Changed ci(dependabot): bump actions/setup-python from 5 to 6 by @dependabot[bot] in https://github.com/teamtomo/torch-grid-utils/pull/22 ci(dependabot): bump actions/checkout from 4 to 6 by @dependabot[bot] in https://github.com/teamtomo/torch-grid-utils/pull/23 feat: add patch grid by @jdickerson95 in https://github.com/teamtomo/torch-grid-utils/pull/24 feat: Add anisotropic mag option to fft freq grid by …

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teamtomo/torch-grid-utils: v0.1.0

A. Yu. Burt, Marten Chaillet, Josh Dickerson, aysecanuenal et autres

What's Changed feat: Add polar coordinate grid by @jdickerson95 in https://github.com/teamtomo/torch-grid-utils/pull/26 Full Changelog: https://github.com/teamtomo/torch-grid-utils/compare/v0.0.10...v0.1.0

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Decreased cytoplasmic crowding via inhibition of ribosome biogenesis can trigger Candida albicans filamentous growth

Antonio Serrano, Charles T.S. Puerner, Louis Chevalier, E. Plumb et autres

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Tolerance to the antifungal drug fluconazole is mediated by tuning cytoplasmic fluidity

E. Plumb, Antonio Serrano, L. Chevalier, Johannes Elferich et autres

Abstract Treatment failure rates for fungal infections cannot be explained simply by increased rates of drug resistance. Antifungal drug tolerance, the ability of a susceptible isolate to grow in the presence of inhibitory drug concentrations, relies upon a broad set of stress …

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Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography

Hoyoung Kim, Cathy J. Spangler, Aya Matsui, Johannes Elferich et autres

Cryoelectron tomography (cryo-ET) enables three-dimensional visualization of molecular structures within tissue and intact cells, providing a powerful tool for studying the spatial organization of biological components at nanometer resolution. Realizing this potential, particularly for submegadalton complexes, is facilitated by fiducial-based labeling. Gold …

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Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model

Lingli Kong, Ximena Zottig, Johannes Elferich, Nikolaus Grigorieff

The exposure of frozen biological samples to the high-energy electron beam in a cryo-electron microscope commonly leads to beam-induced sample motion and distortions. Previously, we described Unblur , software to correct for beam-induced motion based on the alignment of full frames in …

us (code pays fourni par la source)