Lee Kim Swee

Complementary CRISPR screen highlights the contrasting role of membrane-bound and soluble ICAM-1 in regulating antigen-specific tumor cell killing by cytotoxic T cells

Ann-Kathrin Herzfeldt, Marta Puig Gámez, Eva Maria Garrido-Martin, Lukasz Miloslaw Boryn et autres

Cytotoxic CD8 +T lymphocytes (CTLs) are key players of adaptive anti-tumor immunity based on their ability to specifically recognize and destroy tumor cells. Many cancer immunotherapies rely on unleashing CTL function. However, tumors can evade killing through strategies which are not yet …

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Author response: Complementary CRISPR screen highlights the contrasting role of membrane-bound and soluble ICAM-1 in regulating antigen-specific tumor cell killing by cytotoxic T cells

Ann-Kathrin Herzfeldt, Marta Puig Gámez, Eva Maria Garrido-Martin, Lukasz Miloslaw Boryn et autres

Loss of membrane-bound ICAM-1, overexpression of soluble ICAM-1 or ILKAP activity in tumor cells induce resistance to antigen-specific cytotoxic T-cell-mediated killing independently from regulating antigen presentation, PD-L1 expression, IFN-γ, or TNF-α responsiveness.

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Senescent cells suppress macrophage-mediated corpse removal via upregulation of the CD47-QPCT/L axis

Daniela Schloesser, Laura Lindenthal, Julia Sauer, Kyoung‐Jin Chung et autres

Progressive accrual of senescent cells in aging and chronic diseases is associated with detrimental effects in tissue homeostasis. We found that senescent fibroblasts and epithelia were not only refractory to macrophage-mediated engulfment and removal, but they also paralyzed the ability of macrophages …

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Complementary CRISPR screen highlights the contrasting role of membrane-bound and soluble ICAM-1 in regulating antigen specific tumor cell killing by cytotoxic T cells

Ann-Kathrin Mattes, Marta Puig Gámez, Eva Maria Garrido-Martin, Lukasz Miloslaw Boryn et autres

Abstract Cytotoxic CD8+ T lymphocytes (CTLs) are key players of adaptive anti-tumor immunity based on their ability to specifically recognize and destroy tumor cells. Many cancer immunotherapies rely on unleashing CTL function. However, tumors can evade killing through strategies which are not …

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CD206+ tumor-associated macrophages cross-present tumor antigen and drive antitumor immunity

Madhura Modak, Ann-Kathrin Mattes, Daniela Reiss, Wioletta Skrońska-Wąsek et autres

In many solid cancers, tumor-associated macrophages (TAM) represent the predominant myeloid cell population. Antigen (Ag) cross-presentation leading to tumor Ag-directed cytotoxic CD8+ T cell responses is crucial for antitumor immunity. However, the role of recruited monocyte-derived macrophages, including TAM, as potential cross-presenting …

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Sortase-A mediated chemoenzymatic lipidation of single-domain antibodies for cell membrane engineering

Steffen Wöll, Christopher Bachran, Stefan M. Schiller, Lee Kim Swee et autres

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Sortagging of liposomes with a murine CD11b-specific VHH increases in vitro and in vivo targeting specificity of myeloid cells

Steffen Wöll, Christopher Bachran, Stefan M. Schiller, Matthias Schröder et autres

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Sortaggable liposomes: Evaluation of reaction conditions for single-domain antibody conjugation by Sortase-A and targeting of CD11b+ myeloid cells

Steffen Wöll, Christopher Bachran, Stefan M. Schiller, Matthias Schröder et autres

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Genetic screen in myeloid cells identifies TNF-α autocrine secretion as a factor increasing MDSC suppressive activity via Nos2 up-regulation

Matthias Schröder, Marit Krötschel, Lena Conrad, Svenja Kerstin Naumann et autres

The suppressive microenvironment of tumors remains one of the limiting factors for immunotherapies. In tumors, the function of effector T cells can be inhibited by cancer cells as well as myeloid cells including tumor associated macrophages and myeloid-derived suppressor cells (MDSC). A …

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Pentaglycine lipid derivates – rp-HPLC analytics for bioorthogonal anchor molecules in targeted, multiple-composite liposomal drug delivery systems

Steffen Wöll, Stefan M. Schiller, Christopher Bachran, Lee Kim Swee et autres

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The activity of myeloid cell-specific VHH immunotoxins is target-, epitope-, subset- and organ dependent

Christopher Bachran, Matthias Schröder, Lena Conrad, Juan José Cragnolini et autres

The central role of myeloid cells in driving autoimmune diseases and cancer has raised interest in manipulating their function or depleting them for therapeutic benefits. To achieve this, antibodies are used to antagonize differentiation, survival and polarization signals or to kill target …

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Highly sensitive, selective and label-free protein detection in physiological solutions using carbon nanotube transistors with nanobody receptors

Marcin S. Filipiak, Marcel Rother, Nesha May Andoy, Arne C. Knudsen et autres

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