José Luis Sáez

Temporal shifts in antimicrobial resistance phenotypes in indicator and third-generation cephalosporin-resistant Escherichia coli from Spanish livestock during a period of reduced veterinary antimicrobial sales

Carlos Serna, María Ugarte‐Ruiz, Bernabé Diéguez-Roda, Iratxe Pérez-Cobo et autres

Abstract Antimicrobial resistance (AMR) in food-producing animals is a major One Health concern. In Europe, surveillance programs monitor resistance in commensal Escherichia coli as an indicator organism. However, single-drug metrics often overlook co-resistance patterns and links between antimicrobial use (AMU) and multidrug …

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Risk Factors Influencing the Specificity of Skin Tests for Bovine Tuberculosis in a Low-Prevalence Region

Alberto Gómez-Buendía, Roxana Triguero-Ocaña, Marta Muñoz‐Mendoza, Marta Alvarez-Fidalgo et autres

Background: Skin tests are the main official diagnostic method for granting, maintaining, and regaining the officially tuberculosis free (OTF) herd status in the European Union. Although highly specific, repeated testing may lead to the culling of animals not infected with bovine tuberculosis …

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Molecular epidemiology of Salmonella Enteritidis in humans and animals in Spain

Clara Samper-Cativiela, Laura Torre‐Fuentes, Bernabé Diéguez-Roda, Margo Maex et autres

Enteritidis, the most prevalent serovar-causing human gastroenteritis, has been traditionally linked to poultry sources. Although antimicrobial resistance (AMR) is not common in this serovar, increasing levels of resistance to fluoroquinolones and ampicillin have been reported in the last few years. Here, 298 …

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Zoonotic tuberculosis in Catalonia, Spain: Phylogenetic insights into Mycobacterium bovis and M. caprae transmission at the human-livestock interface

Bernat Pérez de Val, Enríc Vidal, Tod Stuber, José Luis Sáez et autres

This genomic epidemiology study analyzed Mycobacterium bovis and M. caprae isolates from animals and humans in Catalonia, Spain, between 2005 and 2023. Of 42 human tuberculosis (TB) cases, five were phylogenetically linked to livestock outbreaks, suggesting zoonotic transmission. The findings specifically indicate …

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Evaluating the ability of non-tuberculous mycobacteria to induce non-specific reactions in bovine tuberculosis diagnostic tests in guinea pigs and cattle

Alberto Gómez-Buendía, Javier Ortega, Alberto Díez‐Guerrier, Aaron Rendahl et autres

Limitations in diagnostic test performance are one of the major challenges hampering the eradication of bovine tuberculosis (bTB). Non-tuberculous mycobacteria (NTM) are considered one of the main causes of non-specific reactions in the intradermal tuberculin test, the most widely used bTB diagnostic …

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Deciphering resistance mechanisms to tigecycline and colistin in Salmonella enterica isolates from animal production

Judith Guitart-Matas, Miquel Sánchez−Osuna, José Luis Sáez, Cristina de Frutos et autres

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Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds

Alberto Gómez-Buendía, Julio Álvarez, Javier Bezos, Jorge Mourelo et autres

Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a …

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Risk Factors for Salmonella Detection in Commercial Layer Flocks in Spain

Clara Samper-Cativiela, Maria Esther Prieto, Soledad Collado, Cristina de Frutos et autres

Trends in Salmonella human infections are assumed to be related to the distribution of the pathogen in the animal reservoir/food products, and cases in humans are most often linked to poultry and poultry products (eggs, meat). Therefore, ongoing Salmonella national control programs …

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Evaluation of the performance of the IFN-γ release assay in bovine tuberculosis free herds from five European countries

Alberto Gómez-Buendía, Beatriz Romero, Javier Bezos, José Luis Sáez et autres

Abstract The diagnostic methods for granting and maintenance of the official tuberculosis-free (OTF) status and for intra-Community movement of cattle are the tuberculin skin tests (single or comparative) and the interferon-γ (IFN-γ) release assay (IGRA). However, until now, IGRAs have been primarily …

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Once bitten twice shy: Risk factors associated with bovine tuberculosis recurrence in Castilla y Leon, Spain

Pilar Pozo, Javier Bezos, Beatriz Romero, Anna Grau et autres

Persistence of bovine tuberculosis (bTB) in cattle herd remains a major challenge in disease elimination due to the ineffectual removal of all infected animals in a bTB breakdown. Characterization of herds with a higher probability of experiencing further bTB breakdowns can help …

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Once Bitten Twice Shy: Risk Factors Associated with Bovine Tuberculosis Recurrence in Castilla Y Leon, Spain

Pilar Pozo, Javier Bezos, Beatriz Romero, Anna Grau et autres

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Additional file 2 of Evaluation of the performance of the IFN-γ release assay in bovine tuberculosis free herds from five European countries

Alberto Gómez-Buendía, Beatriz Romero, Javier Bezos, José Luis Sáez et autres

Additional file 2: Evolution of the animal-level specificity of the Bovigam and IDvet kit depending on the cut-off point (OD or S/P ratio) used to define positivity, depending on different covariables.

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