Paula Haynes

Glucose is dynamically regulated by time of day in humans and Drosophila

Dania Malik, Pinky Kain, Seth D. Rhoades, Arjun Sengupta et autres

Biological clocks shape metabolism, but how circadian programs govern nutrient processing is unclear. Here, using human metabolomics and 13C6-glucose tracing in Drosophila, we delineate previously under characterized daily oscillations in glucose-derived metabolic networks, providing a mechanistic framework for a purpose-built isotope-tracing approach. …

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Author response: Integrated Respirometry and Metabolomics Unveil Circadian Metabolic Dynamics in Drosophila

Farheen Akhtar, Dania Malik, Arjun Sengupta, Paula Haynes et autres

Precise temporal regulation of metabolism by sleep and circadian rhythms is essential for dynamic energy homeostasis, yet the link between systemic metabolism and respiratory demands remains poorly defined. We combined high-resolution respirometry with LC-MS-based metabolomics to characterize respiratory dynamics and metabolic states …

Integrated Respirometry and Metabolomics Unveil Circadian Metabolic Dynamics in Drosophila

Farheen Akhtar, Dania Malik, Arjun Sengupta, Paula Haynes et autres

Precise temporal regulation of metabolism by sleep and circadian rhythms is essential for dynamic energy homeostasis, yet the link between systemic metabolism and respiratory demands remains poorly defined. We combined high-resolution respirometry with LC-MS-based metabolomics to characterize respiratory dynamics and metabolic states …

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Integrated Respirometry and Metabolomics Unveil Circadian Metabolic Dynamics in Drosophila

Farheen Akhtar, Dania Malik, Arjun Sengupta, Paula Haynes et autres

Sleep and circadian rhythms shape organismal energy patterns, but how this timing connects to oxygen use and carbon dioxide production remains incompletely understood. We combined high-resolution respirometry with LC-MS-based metabolomics to characterize respiratory dynamics and metabolic states in Drosophila melanogaster, resolving genotype-specific …

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Neutral lipid processing in glia is sexually dimorphic and promotes sleep through diacylglycerol catabolism

Elana S. Pyfrom, Connor Beveridge, Paula Haynes, Vishal A. Kanigicherla et autres

Abstract Sleep is thought to have a protective role in clearing toxic waste from the brain, which may include processing of damaged lipids. We recently showed that blocking endocytosis in glia increases sleep and report here that this block is associated with …

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Integrated Respirometry and Metabolomics Unveil Circadian Metabolic Dynamics in Drosophila

Farheen Akhtar, Dania Malik, Arjun Sengupta, Paula Haynes et autres

ABSTRACT Sleep and circadian rhythms shape organismal energy patterns, but how this timing connects to oxygen use and carbon dioxide production remains incompletely understood. We combined high-resolution respirometry with LC-MS-based metabolomics to characterize respiratory dynamics and metabolic states in Drosophila melanogaster , …

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A neuron–glia lipid metabolic cycle couples daily sleep to mitochondrial homeostasis

Paula Haynes, Elana S. Pyfrom, Yongjun Li, Carly Stein et autres

Sleep is thought to be restorative to brain energy homeostasis, but it is not clear how this is achieved. We show here that Drosophila glia exhibit a daily cycle of glial mitochondrial oxidation and lipid accumulation that is dependent on prior wake …

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Glucose Challenge Uncovers Temporal Fungibility of Metabolic Homeostasis over a day:night cycle

Dania Malik, Seth D. Rhoades, Shirley Zhang, Arjun Sengupta et autres

Abstract Rhythmicity is a cornerstone of behavioral and biological processes, especially metabolism, yet the mechanisms behind metabolite cycling remain elusive. This study uncovers a robust oscillation in key metabolite pathways downstream of glucose in humans. A purpose-built 13 C 6 -glucose isotope …

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Ecdysone acts through cortex glia to regulate sleep in Drosophila

Yongjun Li, Paula Haynes, Shirley Zhang, Zhifeng Yue et autres

Steroid hormones are attractive candidates for transmitting long-range signals to affect behavior. These lipid-soluble molecules derived from dietary cholesterol easily penetrate the brain and act through nuclear hormone receptors (NHRs) that function as transcription factors. To determine the extent to which NHRs …

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Author response: Ecdysone acts through cortex glia to regulate sleep in Drosophila

Yongjun Li, Paula Haynes, Shirley Zhang, Zhifeng Yue et autres

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Ecdysone acts through cortex glia to regulate sleep in Drosophila

Yongjun Li, Paula Haynes, Shirley Zhang, Zhifeng Yue et autres

Summary Steroid hormones are attractive candidates for transmitting long-range signals to affect behavior. These lipid-soluble molecules derived from dietary cholesterol easily penetrate the brain and act through nuclear hormone receptors (NHRs) that function as transcription factors. To determine the extent to which …

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Availability of food determines the need for sleep in memory consolidation

Nitin S. Chouhan, Leslie C. Griffith, Paula Haynes, Amita Sehgal

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