Lili Cui

Lyophilizable Thermostable Nano‐Aluminum Adjuvant and Combinations Induced Robust Antigen‐Specific Humoral and Cellular Immunity in Mice

Jingying Zhou, Lijun Bian, X F Zhao, Lili Cui et autres

Aluminum adjuvants have been used in licensed human vaccines for almost a century. However, they can neither induce an efficient cellular immune response, nor be frozen or lyophilized due to aggregation and diminished adjuvant efficacy. This study developed a series of lyophilizable …

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The interplay between cationic peptide sequence, siRNA and electrolyte: formulating lipopolyplexes in NaCl solution affords nanocomplexes with exceptional levels of gene silencing

Lili Cui, Laila Kudsiova, Frederick Campbell, David J. Barlow et autres

Formulating siRNA lipopolyplexes in NaCl solution gives large, multilamellar complexes with high levels of gene silencing in the presence of serum.

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Deciphering the biological fate of mRNA-LNP-based biologics: A perspective from tissue to intracellular distribution

Xin Xu, Lili Cui, Yong Zhang, Jingkai Gu

The remarkable clinical success of mRNA-lipid nanoparticle (mRNA-LNP) vaccines, particularly evidenced by their pandemic-era impact and subsequent 2023 Nobel Prize recognition, has intensified global efforts to expand their application toward therapeutic biologics. Despite showing strong preventive effects, there are still fundamental knowledge …

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Biodistribution and mechanisms of action of MF59 and MF59-like adjuvants

Wen Zhang, Jie Xu, Lijun Bian, Lili Cui et autres

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Heterologous Prime-Boost Immunization Strategies Using Varicella-Zoster Virus gE mRNA Vaccine and Adjuvanted Protein Subunit Vaccine Triggered Superior Cell Immune Response in Middle-Aged Mice

Dongdong Li, Lijun Bian, Lili Cui, Jingying Zhou et autres

Purpose: Heterologous immunization using different vaccine platforms has been demonstrated as an efficient strategy to enhance antigen-specific immune responses. In this study, we performed a head-to-head comparison of both humoral and cellular immune response induced by different prime-boost immunization regimens of mRNA …

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Understanding Intracellular Biology to Improve mRNA Delivery by Lipid Nanoparticles

Morag Rose Hunter, Lili Cui, Benjamin T. Porebski, Sara G. T. Pereira et autres

Poor understanding of intracellular delivery and targeting hinders development of nucleic acid-based therapeutics transported by nanoparticles. Utilizing a siRNA-targeting and small molecule profiling approach with advanced imaging and machine learning biological insights is generated into the mechanism of lipid nanoparticle (MC3-LNP) delivery …

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Understanding and optimising the transfection of lipopolyplexes formulated in saline: the effects of peptide and serum

Lili Cui, Laila Kudsiova, Frederick Del Gaudio Campbell, David J. Barlow et autres

Lipopolyplexes formulated from branched cationic peptides with cell receptor targeting sequences, DOTMA and DOPE, and plasmid DNA in the presence of saline form multilamellar nanoparticles with enhanced stability and transfection in serum.

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Nucleic Acid-Loaded Lipid Nanoparticle Interactions with Model Endosomal Membranes

Alice Spadea, Mark J. Jackman, Lili Cui, Sara G. T. Pereira et autres

Lipid nanoparticles (LNPs) are important delivery systems for RNA-based therapeutics, yet the mechanism of their interaction with endosomal membranes remains unclear. Here, the interactions of nucleic acid-loaded LNPs that contain an ionizable lipid with models of the early and late endosomal membranes …

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Development of a high-throughput platform for screening lipid nanoparticles for mRNA delivery

Lili Cui, Sara G. T. Pereira, Silvia Sonzini, Sally van Pelt et autres

. Moreover, this platform has been integrated into a fully-automated workflow for LNP property control, physicochemical characterisation and biological evaluation. The high-throughput platform may accelerate proprietary lipid development, mRNA LNP lead optimisation and candidate selection to advance preclinical mRNA LNP development to …

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Mechanistic Studies of an Automated Lipid Nanoparticle Reveal Critical Pharmaceutical Properties Associated with Enhanced mRNA Functional Delivery In Vitro and In Vivo

Lili Cui, Morag Rose Hunter, Silvia Sonzini, Sara G. T. Pereira et autres

Recently, lipid nanoparticles (LNPs) have attracted attention due to their emergent use for COVID-19 mRNA vaccines. The success of LNPs can be attributed to ionizable lipids, which enable functional intracellular delivery. Previously, the authors established an automated high-throughput platform to screen ionizable …

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Synthesis of Janus Fe3O4&mSiO2 Nanocarriers for Chemo-Microwave Therapy of Cancer Cells

Ye Liu, Vignesh Murugadoss, Cui Bin, Dejian Tang et autres

Hollow Bimetallic Complex Nanoparticles for Trimodality Imaging and Photodynamic Therapy In Vivo

Tianli Zhai, Chenchen Wang, Lili Cui, Jing Du et autres

Hollow nanoparticles have received an enormous amount of attention in the field of nanomedicine. Herein, water-soluble hollow bimetallic complex nanoparticles, holmium(III)/iridium(III) bimetallic complex nanoparticles (Ir-Ho HNPs), were fabricated via a coordination assembly. Owing to the special metal-to-ligand charge transfer (MLCT) and the …

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