Thierry Naas

Development of simplex and multiplex immunochromatographic assays for rapid detection of GES-, PER-, and VEB-producing gram-negative bacteria

Melisa Nicevic, Christian Moguet, Saoussen Oueslati, Stéphanie Gelhaye et autres

ABSTRACT Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are increasingly reported worldwide, emphasizing the need for rapid diagnostic tools to limit their spread and prevent outbreaks. Although CTX-M enzymes remain predominant, less common ESBLs such as GES, PER, and VEB are emerging and gaining clinical …

ba, fr, us (code pays fourni par la source)

Reduced Susceptibility to Cefiderocol Among Clinical MCR-1-Producing Escherichia coli Isolates from Tunisia

Nadia Jaidane, Thierry Naas, Souad Fayad, Pierre Châtre et autres

Background/Objectives: The emergence of plasmid-mediated mcr genes has enabled horizontal dissemination of resistance to colistin, a last-resort antibiotic against multidrug-resistant Enterobacterales. In Tunisia, genomic data on mcr-positive Escherichia coli are still limited. This study reports the genomic characterization of human clinical mcr-positive …

Tunisie, fr, ps (code pays fourni par la source)

Epidemiology of KPC-producing Enterobacterales isolated from 2019 to 2023 in France

Lamia Rouabah, Sarah Ronsin, Axelle Vasseur, Inès Rezzoug et autres

OBJECTIVES: The global spread of Klebsiella pneumoniae carbapenemase (KPC)-type carbapenemases has historically been driven by clonal group CG258, but increasing clonal diversification has recently been reported. We aimed to provide a comprehensive phenotypic and genomic characterization of KPC-producing Enterobacterales referred to French …

fr (code pays fourni par la source)

Genomic characterization of a Klebsiella pneumoniae ST231 clinical isolate from Tunisia co-producing mcr-1.1 and blaOXA-181

Nadia Jaidane, Souad Fayad, Manel Ennaceur, Pierre Châtre et autres

Objective Plasmid-encoded colistin resistance mcr-1 genes are mainly reported in Escherichia coli worldwide. Here, we characterized a multidrug-resistant Klebsiella pneumoniae 70241 clinical isolate harbouring mcr-1.1 and bla OXA-181 , recovered from a tertiary-care hospital in Tunis, Tunisia. Methods Antimicrobial susceptibility was tested …

Tunisie, fr (code pays fourni par la source)

Wildlife as a reservoir of OXA-48-like carbapenemase-producing Enterobacterales

Imene Mehidi, Alima Sait-Gharout, Abdelazize Franck Bougaham, Hocine Gougam et autres

ABSTRACT Carbapenemase-producing Enterobacterales (CPEs) have globally emerged and spread beyond human compartments. However, data in wild animals, especially from low- and middle-income countries, such as Algeria, are still very scarce. Here, we investigated CPEs recovered from feces samples collected between October 2021 …

fr, Algérie, us (code pays fourni par la source)

Characterization of GUA-1, a chromosomally encoded ESBL from Pseudomonas guariconensis-like

Saoussen Oueslati, Nadia Jaidane, Réva Nermont, Delphine Girlich et autres

ABSTRACT Putative novel β-lactamase-encoding genes are increasingly identified in whole-genome sequencing (WGS) data, often without phenotypic or biochemical characterization. Here, we characterized the chromosome-encoded GUA-1 Ambler class A β-lactamase from the clinical isolate Pseudomonas guariconensis-like 65411. Among the 40 P. guariconensis genomes …

fr, Tunisie, ps, us (code pays fourni par la source)

Reply to Feng, “Recalculation of inhibitor concentration fold-changes for CMY-219: comments on a recent article about CMY-219 conferring resistance to ceftazidime-avibactam”

Agnès B. Jousset, Delphine Girlich, Saoussen Oueslati, Aurélien Birer et autres

fr (code pays fourni par la source)

Evaluation of a Novel In Vitro Diagnostic Immunoassay for the Rapid Qualitative Detection of KPC, NDM, OXA-48-like, IMP and VIM Carbapenemases

Zoe Dunne, Saoussen Oueslati, Hervé Volland, Thierry Naas

Background/Objectives: The global spread of carbapenemase-producing Gram-negative bacteria (CP-GNB) represents a major clinical challenge, causing severe hospital-acquired infections with limited treatment options. Accurate and rapid detection is essential for guiding antimicrobial therapy and implementing infection control measures. Lateral flow immunoassays (LFIAs) targeting …

ie, fr (code pays fourni par la source)

Emergence and characterization of new OXA-181 variants in France: OXA-1181, OXA-1201, OXA-1205, OXA-1207, and OXA-1226

Léa Bientz, Réva Nermont, Cécile Emeraud, Thierry Naas et autres

ABSTRACT Since 2022, the diversity of OXA-48 variants has increased in France. We analyzed the dissemination of five recent variants (OXA-1181, OXA-1201, OXA-1205, OXA-1207, and OXA-1226) mostly identified on plasmids in high-risk Escherichia coli clones. Three of them (OXA-1181, OXA-1205, and OXA-1207) …

fr, us (code pays fourni par la source)

Evidence of transmission and dissemination of diverse bla NDM-5 -producing Escherichia coli clones between refugee and host communities and their environment: a multicenter cross-sectional study

Souad Fayad, Dina Daaboul, Issmat I. Kassem, Aula Abbara et autres

ABSTRACT The global spread of carbapenemase-producing Escherichia coli (CP-Ec) poses a significant public health threat, with particularly severe consequences for vulnerable populations in resource-limited settings. To address this, we conducted in-depth genetic analyses and examined the relatedness of CP-Ec isolates recovered from …

fr, lb, us, ge, gb, sy (code pays fourni par la source)

Exploring Malaysian biodiversity in the fight against antibiotic resistance

Saoussen Oueslati, Cécile Apel, Thierry Naas, Marc Litaudon et autres

fr (code pays fourni par la source)

Comparison of Two Lateral Flow Immunochromatographic Assays for Rapid Detection of KPC, NDM, IMP, VIM and OXA-48 Carbapenemases in Gram-Negatives

Saoussen Oueslati, Nahed Al Laham, Réva Nermont, Hervé Volland et autres

The spread of carbapenemase-producing Gram-negative bacteria poses a significant clinical challenge due to their association with severe Difficult-to-Treat nosocomial infections, as available therapies are drastically reduced. Rapid and accurate detection of carbapenemase-producing Gram-negative bacteria is critical for effective patient management, guiding appropriate …

es, fr, ps (code pays fourni par la source)