Hiroyuki D. Sakai

A Tripartite Co-culture System Reveals Defensive Mutualism Between a Nanobdellati Symbiont and Its Host

Hiroyuki D. Sakai, Sabine Schwarzer, Satoshi Nakagawa, Milad Reyhani et autres

Archaeal symbioses remain among the least understood cellular interactions. Ultra-small Nanobdellati (initially called DPANN) archaea rely on larger hosts for survival, yet their effects on host eco-physiology and their interactions with infecting viruses have not yet been examined experimentally. Here, we established …

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Caldiplasma sukawensis gen. nov., sp. nov., and Cuniculiplasma thermophilum sp. nov., mesophilic, acidophilic, cell–wall–less, archaea of the order Thermoplasmatales, isolated from extremely acidic hot spring

Yuya Tsukamoto, Hiroyuki D. Sakai, Yoshinori Takano, Toshiki Koga et autres

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Microcaldota

Hiroyuki D. Sakai, Takashi Itoh, Shingo Kato, Naswandi Nur et autres

Abstract Mi.cro.cal.do'ta. N.L. masc. n. Microcaldus , type genus of the class Microcaldia; L. neut. pl. n. suff. ‐ota , ending to denote a phylum; N.L. neut. pl. n. Microcaldota , the phylum of the class Microcaldia . The phylum Microcaldota , …

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Microcaldus

Hiroyuki D. Sakai, Takashi Itoh, Shingo Kato, Matthew D. Johnson et autres

Abstract Mi.cro.cal'dus. Gr. masc. adj. mikros , small (may keep ‐o); L. masc. adj. caldus , hot; N.L. masc. n. Microcaldus , a thermophilic small‐size microorganism. The genus Microcaldus currently includes a single species, Microcaldus variisymbioticus . It is an obligately symbiotic …

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Complete genome sequence of strain KD-1 T , the type strain of Sulfurisphaera javensis , isolated from an Indonesian hot spring

Hiroyuki D. Sakai, Naswandi Nur, Antonius Suwanto, Norio Kurosawa

ABSTRACT Sulfurisphaera javensis KD-1 T , originally isolated from an Indonesian acidic hot spring, is a hyperthermophilic, acidophilic, and facultatively anaerobic archaeon. Here, we report the 2.6 Mb complete genome sequence of KD-1 T . Genes related to sulfur metabolism were found …

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N -linked protein glycosylation in Nanobdellati (formerly DPANN) archaea and their hosts

Satoshi Nakagawa, Hiroyuki D. Sakai, Shigeru Shimamura, Yoshiki Takamatsu et autres

ABSTRACT Members of the kingdom Nanobdellati , previously known as DPANN archaea, are characterized by ultrasmall cell sizes and reduced genomes. They primarily thrive through ectosymbiotic interactions with specific hosts in diverse environments. Recent successful cultivations have emphasized the importance of adhesion …

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Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system

Matthew D. Johnson, Doulin C. Shepherd, Hiroyuki D. Sakai, Manasi Mudaliyar et autres

DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for proliferation. The structural and molecular details of …

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Novel cell-to-cell interactions revealed by cryotomography of a DPANN coculture system

Matthew D. Johnson, Doulin C. Shepherd, Hiroyuki D. Sakai, Manasi Mudaliyar et autres

Abstract DPANN is a widespread and highly diverse group of archaea characterised by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for their survival and proliferation. Despite …

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A large attachment organelle mediates interaction between a novel Nanobdellota archaeon YN1 and its host

Matthew D. Johnson, Hiroyuki D. Sakai, Bindusmita Paul, Takuro Nunorura et autres

DPANN archaea are an enigmatic superphylum that are difficult to isolate and culture in the laboratory due to their specific culture conditions and apparent ectosymbiotic lifestyle. Here we successfully isolated and cultivated a co-culture system of a novel Nanobdellota archaeon YN1 and …

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Efficacy and safety of nemolizumab and topical corticosteroids for prurigo nodularis: results from a randomized double-blind placebo-controlled phase II/III clinical study in patients aged ≥ 13 years

Hiroo Yokozeki, Hiroyuki Murota, Takayo Matsumura, Hiroshi Komazaki et autres

BACKGROUND: Prurigo nodularis (PN), a chronic inflammatory skin condition, adversely affects the quality of life of affected individuals. Current treatment options for PN in Japan are limited. OBJECTIVES: To evaluate the optimal dose, efficacy and safety of long-term treatment with nemolizumab in …

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Characterization of protein glycosylation in an Asgard archaeon

Satoshi Nakagawa, Hiroyuki Imachi, Shigeru Shimamura, Saeko Yanaka et autres

Archaeal cells are typically enveloped by glycosylated S-layer proteins. Archaeal protein glycosylation provides valuable insights not only into their adaptation to their niches but also into their evolutionary trajectory. Notably, thermophilic Thermoproteota modify proteins with N-glycans that include two GlcNAc units at …

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Large attachment organelle mediates interaction between Nanobdellota archaeon YN1 and its host

Matthew D. Johnson, Hiroyuki D. Sakai, Bindusmita Paul, Takuro Nunoura et autres

DPANN archaea are an enigmatic superphylum that are difficult to isolate and culture in the laboratory due to their specific culture conditions and apparent ectosymbiotic lifestyle. Here, we successfully isolated and cultivated a coculture system of a novel Nanobdellota archaeon YN1 and …

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