Yangyang Chang

Advances in aptamer-based sensors for environmental small-molecule pollutant detection

Chengchen Li, Yanan Wang, Rui Zhang, Ying Zhang et autres

DNAzyme-Based Core–Shell Hydrogel Microneedle Arrays for In Situ Live Bacterial Detection on Surfaces

Wei Xue, Tao Sheng, Yangyang Chang, Xiaozheng Li et autres

Surface bacterial contamination poses significant risks to public health and food safety, yet conventional detection methods often require complex sample processing and laboratory equipment. Here, we report simple and sensitive DNAzyme-based core–shell hydrogel microneedle arrays (DzCS-HMA) for in situ live bacterial detection …

cn (code pays fourni par la source)

Unlocking the hidden DNAzyme repertoire through aptamer-gated exonuclease signal amplification

Shusen Xiao, Kai Niu, Yangyang Chang, Zijie Zhang et autres

We present an aptamer-gated exonuclease signal amplification (AGESA) strategy decoupling target recognition from DNAzyme activation to overcome background limitations, enabling selective detection of platelet-derived growth factor BB in urine and unlocking the DNAzyme repertoire for protein biomarkers.

cn (code pays fourni par la source)

DNAzyme-Based Point-of-Care Diagnosis of Aggressive Periodontitis

Li Xia, Shusen Xiao, Zijie Zhang, Dan Xue et autres

(AA), a key pathogen in aggressive periodontitis, remains a critical challenge in point-of-care diagnostics. Here, we report the development of a portable lateral flow device (LFD) using an RNA-cleaving DNAzyme (RCD-AA5) as a highly specific molecular recognition element. Selected via systematic evolution …

cn (code pays fourni par la source)

Research and application of ceramic identification and traceability methods based on SIFT and blockchain

Yangyang Chang, Jingfang Hu, Xien Cheng

To address the challenges in ceramic artwork authentication and provenance—such as reduced matching precision caused by edge noise and outliers and the high storage cost of blockchain systems—we propose a hybrid SIFT (Scale Invariant Feature Transform)-Canny-Adaptive Locally-Affine Matching (AdaLAM) algorithm integrated with …

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Site‐Specific Recognition of Inosine‐Modified RNA by Deoxyribozyme with Distant Cleavage Site

Shusen Xiao, Yangyang Chang, Xiaoqian Li, Li Xia et autres

Abstract We report an RNA‐cleaving DNAzyme (RCD), RCD‐I4T3, discovered through in vitro selection, that can specifically recognize an inosine‐modified site in RNA and cleave at a remote site (eight nucleotides downstream of the inosine). RCD‐I4T3 represents the first DNAzyme with spatially separated …

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Site‐Specific Recognition of Inosine‐Modified RNA by Deoxyribozyme with Distant Cleavage Site

Shusen Xiao, Yangyang Chang, Xiaoqian Li, Li Xia et autres

Abstract We report an RNA‐cleaving DNAzyme (RCD), RCD‐I4T3, discovered through in vitro selection, that can specifically recognize an inosine‐modified site in RNA and cleave at a remote site (eight nucleotides downstream of the inosine). RCD‐I4T3 represents the first DNAzyme with spatially separated …

cn (code pays fourni par la source)

Click DNA ligation with deoxyribozyme

Yangyang Chang, Liang Yu, Haodong Song, Qiang Zhang et autres

Deoxyribozymes that ligate DNA will expand the reaction scope of DNA catalysis and are useful in the construction of DNA nanostructures. Herein, we report the first efforts to isolate a novel class of DNA ligase deoxyribozymes from a random sequence DNA pool …

cn, Éthiopie (code pays fourni par la source)

Integrating Fluorogenic DNAzymes with Photonic Crystals for Bacterial Detection on a Microfluidic Biochip

Rui Zhang, Chen Ying An, Jiuxing Li, Xiaozheng Li et autres

Conventional bacterial detection relies on culture-based methods and nucleic acid amplification. These multistep procedures increase complexity, processing time, and cost, limiting their utility in point-of-care (POC) diagnostics. To address this, we developed fluorogenic DNAzymes (FDz) for rapid bacterial detection on a photonic-crystal-assisted …

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Engineering Acidic Deoxyribozyme with Strongly Cooperative H+ Binding for Subcellular Imaging

Q. W. Meng, Qiang Zhang, Rui Zhang, Zijie Zhang et autres

Engineering deoxyribozymes under extreme conditions will expand their repertoires for unique applications. Although all known deoxyribozyme-based biosensors were derived to function under or near physiological conditions, no prior study has been conducted to purposely build them to absolutely function under atypical conditions. …

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Arrest of CRISPR-Cas12a by Nonspecific Single-Stranded DNA for Biosensing

Yue Liu, Yunping Wu, Yi Liu, Qiang Zhang et autres

CRISPR-Cas technologies have emerged as powerful biosensing tools for the sensitive and specific detection of non-nucleic acid targets. However, existing biosensing strategies suffer from poor compatibility across diverse targets due to the complicated engineering of crRNA and DNA activator required for the …

cn, pl (code pays fourni par la source)

Contributors

Zoha Babaei Afrapoli, Arafeh Bigdeli, Jijuan Cao, Yangyang Chang et autres