Iestyn Pope

Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

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Reviewer #1 (Public review): Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Reviewer #2 (Public review): Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Reviewer #3 (Public review): Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Author response: Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

ca, gb (code pays fourni par la source)

Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Abstract Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6 -deficient (G ata6 -KO mye ) mice, significant …

ca, gb (code pays fourni par la source)

Correlative Extinction and Single Fluorophore Bleaching Microscopy for Ligand Quantification on Gold Nanoparticles

Nicole Slesiona, Lukas Payne, Iestyn Pope, Paola Borri et autres

Abstract Nanoparticles (NPs) are promising therapeutic delivery agents, with the number and manner of presentation of cell‐binding ligands on the NP affecting the eventual fate of the therapeutic. Whenever NPs are conjugated with biomolecules, a heterogeneous population of decorated NPs will be …

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Background-free four-wave mixing microscopy of small gold nanoparticles inside a multi-cellular organ

Iestyn Pope, Nuno G.C. Ferreira, Peter Kille, W. Langbein et autres

The ability to detect small metallic nanoparticles by optical microscopy inside environmentally relevant species may have a wide impact for ecotoxicology studies. Here, we demonstrate four-wave mixing microscopy on individual small gold nanoparticles inside the hepatopancreas of Oniscus Asellus, a terrestrial isopod, …

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Correlative light-electron microscopy using small gold nanoparticles as single probes

Iestyn Pope, Hugh Tanner, Francesco Masia, Lukas Payne et autres

Correlative light-electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe. Individual gold nanoparticles bound to the epidermal growth factor …

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Correlative extinction and single fluorophore bleaching microscopy for ligand quantification on gold nanoparticles

Nicole Slesiona, Lukas Payne, Iestyn Pope, Paola Borri et autres

Nanoparticles (NPs) are promising therapeutic delivery agents, yet it is increasingly apparent that the number and manner of presentation of cell binding ligands on the NP can affect the eventual fate of the therapeutic. Whenever NPs are conjugated with biomolecules, a heterogenous …

Correlative light electron microscopy using small gold nanoparticles as single probes

Iestyn Pope, Hugh Tanner, Francesco Masia, Lukas Payne et autres

Correlative light electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe. Individual gold nanoparticles bound to the epidermal growth …