Accès ouvert
2026
article
OpenAlex
D. Regan, Ozan Aksakal, John J.K. McLarnon, Athena Zitti et autres
Abstract Stimulated Raman scattering (SRS) microscopy offers great potential to surpass fluorescent-based approaches, owing to the sharp line width of Raman vibrations amenable to supermultiplex cell imaging, but currently lacks one crucial component: genetically encodable tags equivalent to fluorescent proteins. Here, we …
gb
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Athena Zitti, Ozan Aksakal, Danoo Vitsupakorn, Pierre J. Rizkallah et autres
The red fluorescent protein mCherry is one of the most widely used fluorescent proteins in biology. Here, we have changed the chromophore chemistry by converting the thioether group of M66 to a thiol group through mutation to cysteine. The new variant, termed …
gb
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Ozan Aksakal, Danoo Vitsupakorn, P.J. Rizkallah, James A. Platts et autres
Abstract The red fluorescent protein mCherry is one of the most utilised fluorescent proteins in biology. Here, we have changed the chromophore chemistry by converting the thioether group of M66 to a thiol group through mutation to cysteine. The new variant termed …
gb
(code pays fourni par la source)
2023
conference-paper
OpenAlex
Owen C. Evans, Vijay Pal Singh, Ozan Aksakal, D. R. Jones et autres
Ideal bioimaging probes in fluorescence microscopy are bright, biocompatible, offer a large signal-to-noise ratio against autofluorescence, and can be specifically attached to target biomolecules of interest. Fluorescent proteins have been engineered towards this goal. However, a limitation of fluorescent proteins is the …
gb
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Husam Sabah Auhim, Bella L. Grigorenko, Tessa K. Harris, Ozan Aksakal et autres
Fluorescent proteins (FPs) have revolutionised the life sciences, but the chromophore maturation mechanism is still not fully understood. Here we photochemically trap maturation at a crucial stage and structurally characterise the intermediate.
iq, gb, ru, us, ca
(code pays fourni par la source)