Accès ouvert
2025
article
OpenAlex
Jacob S. Jordan, Conner C. Harper, Fan Zhang, Esther Kofman et autres
Protein aggregation is a factor in a multitude of neurodegenerative diseases and aggregates of protein-based biotherapeutics can cause toxicity in vivo and adverse patient outcomes. Monoclonal antibody (M)–fluorophore (F) complexes with four different antibody sequences and masses of ∼680 kDa were analyzed …
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2024
article
OpenAlex
Akash M. Patel, Aarron T. Willingham, Alan C. Cheng, Daniela M. Tomazela et autres
Toll-like receptors 7 and 8 are involved in modulating the adaptive and innate immune responses, and their activation has shown promise as a therapeutic strategy in the field of immuno-oncology. While systemic exposure to TLR7/8 agonists can result in poor tolerance, combination …
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Accès ouvert
2024
article
OpenAlex
Jacob S. Jordan, Conner C. Harper, Fan Zhang, Esther Kofman et autres
Aggregation of protein-based therapeutics can occur during development, production, or storage and can lead to loss of efficacy and potential toxicity. Native mass spectrometry of a covalently linked pentameric monoclonal antibody complex with a mass of ∼800 kDa reveals several distinct conformations, …
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Accès ouvert
2023
article
OpenAlex
Tian Xu, Fan Zhang, Daoyang Chen, Liangliang Sun et autres
Production of site-specific cysteine-engineered antibody-drug conjugates (ADCs) in mammalian cells may produce developability challenges, fragments, and heterogenous molecules, leading to potential product critical quality attributes in later development stages. Liquid phase chromatography with mass spectrometry (LC-MS) is widely used to evaluate antibody …
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2023
article
OpenAlex
Yi Yang, Fan Zhang, Yutian Gan, Hui‐Min Zhang et autres
Characterization of antibody charge heterogeneity is an important task for antibody drug development. Recently, a correlation between acidic charge heterogeneity and metal-catalyzed oxidation has been observed for antibody drugs. However, to date, the acidic variants induced by metal-catalyzed oxidation have not been …
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