Chiara Villa

Circulating Placental Growth Factor as a Prognostic Biomarker in High-Risk Glioblastoma Patients

Filippo Gagliardi, Francesca Roncelli, Silvia Snider, Pierfrancesco De Domenico et autres

Background/Objectives: Angiogenesis in glioblastoma (GBM) is a multifactorial process, and blood–brain barrier disruption enables the detection of circulating mediators. The clinical relevance of circulating placental growth factor (PlGF) in GBM remains unclear. This study aimed to investigate the role of PlGF in …

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Impact of flow cytometry-based sorting on microRNA signature of extracellular vesicles derived from mesenchymal stromal cells: a proof-of-concept study

Enrico Ragni, Monica Romanò, Michela Taiana, Daniela Boselli et autres

Aim: This proof-of-concept study aimed to evaluate the impact of fluorescence-based sorting on the microRNA (miRNA) molecular profile of extracellular vesicles (EVs) derived from adipose-derived mesenchymal stromal cells (ASCs) (ASC-EVs), with a focus on osteoarthritis (OA) as a model disease. Methods: ASCs …

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Unravelling Plasma Extracellular Vesicle Diversity With Optimised Spectral Flow Cytometry

Daniela Boselli, Francesca Clemente, Simona Di Terlizzi, Christina Pagiatakis et autres

ABSTRACT Extracellular vesicles (EVs) are crucial for intercellular communication and are found in various biological fluids. The identification and immunophenotyping of such small particles continue to pose significant challenges. Here, we have developed a workflow for the optimisation of a next‐generation panel …

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Setting a Successful Sorting for Extracellular Vesicle Isolation

Monica Romanò, Daniela Boselli, Enrico Ragni, Laura de Girolamo et autres

Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) contain a set of microRNAs with regenerative and anti-inflammatory roles. Therefore, purified MSC-EVs are envisioned as a next-generation therapeutic option for a wide array of diseases. In this protocol, we report the strategy …

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Correction: Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

Daniela Boselli, Maddalena Panigada, Simona Di Terlizzi, Monica Romanò et autres

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Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

Daniela Boselli, Maddalena Panigada, Simona Di Terlizzi, Monica Romanò et autres

BACKGROUND: Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA …

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Time- and Cost-effective Production of Untagged Recombinant Mva by Flow Virometry and Direct Virus Sorting

Boselli Daniela, Maddalena Panigada, Simona Di Terlizzi, Monica Romanò et autres

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Workflow for high-dimensional flow cytometry analysis of T cells from tumor metastases

Cristina Faccani, Gianluca Rotta, Francesca Clemente, Maya Fedeli et autres

We describe a multi-step high-dimensional (HD) flow cytometry workflow for the deep phenotypic characterization of T cells infiltrating metastatic tumor lesions in the liver, particularly derived from colorectal cancer (CRC-LM). First, we applied a novel flow cytometer setting approach based on single …

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Progression of brain white matter hyperintensities in asymptomatic patients with carotid atherosclerotic plaques and no indication for revascularization

Enrico Ammirati, Francesco Moroni, Marco Magnoni, Maria A. Rocca et autres

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Carotid artery plaque uptake of 11C-PK11195 inversely correlates with circulating monocytes and classical CD14++CD16− monocytes expressing HLA-DR

Enrico Ammirati, Francesco Moroni, Marco Magnoni, Elena Busnardo et autres

We explored the relation between blood concentrations of monocyte/lymphocyte subsets and carotid artery plaque macrophage content, measured by positron emission tomography (PET) with 11C-PK11195. In 9 patients with carotid plaques we performed 11C-PK11195-PET/computed tomography angiography imaging and measurement of absolute concentrations and …

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Transcriptome Analysis Identified Significant Differences in Gene Expression Variability Between WM and IgM-MGUS BM B Cell Clones

Alessandra Trojani, Milena Lodola, Alessandra Tedeschi, Antonino Greco et autres

Abstract In this study we focused on determining the transcriptome differences between Waldenström's Macroglobulinemia (WM) and IgM Monoclonal Gammopathy of Undetermined Significance (IgM-MGUS) by gene expression profiling (GEP) considering all the different transcript isoforms of genes that map the human transcriptome (coding …

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Circulating CD14+ and CD14highCD16− classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery

Enrico Ammirati, Francesco Moroni, Marco Magnoni, Simona Di Terlizzi et autres

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