Joel D. Boerckel

Cytoskeletal disassembly by optogenetic control of RhoA signaling termination

Erin E. Berlew, Jude Barakat, Paula Camacho, Joel D. Boerckel

ABSTRACT Cellular morphodynamics require adaptive cytoskeletal remodeling, mediated by precisely coordinated activation and termination of RhoA GTPase signaling. RhoA activation is well-studied, but the kinetics and molecular basis of signaling termination remain poorly understood. We engineered an optogenetic toolbox on the single-component …

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Oxygels : Tunable Phosphorescent Hydrogels for Cherenkov‐Excited Luminescence Imaging (CELI) of Oxygen

Simin Belali, Marien Iliza Ochoa Mendoza, Matthew S. Reed, Danylo Mykhalchuk et autres

We present a family of phosphorescent hydrogels, termed Oxygels, for local deep-tissue oxygen sensing by Cherenkov-excited luminescence imaging (CELI). Oxygels comprise dendritic phosphorescent probes covalently bound within a polyethyleneglycol (PEG) matrix held together by an optically neutral dendritic cross-linker. Covalent attachment of …

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Osteocyte Perilacunar/canalicular Remodeling (PLR) Drives Spatially Heterogeneous Lacunar Remodeling During and After Lactation

Xiaoyu Xu, Yilu Zhou, Wonsae Lee, Srijani Datta et autres

Background: Pregnancy and lactation impose substantial demands on maternal calcium homeostasis, leading to pronounced skeletal remodeling during lactation followed by recovery after weaning. Although bone mass is largely returned at the tissue level after weaning, it remains unclear whether osteocyte-level remodeling exhibits …

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Degeneration-Inspired Architectural States Defined by Voronoi Point Spacing and Surface-Mediated Rescue of Osteogenic Dysfunction in 3D-Printed Scaffolds

J.F. Carpenter, Pratheesh Kanakarajan Vijaya Kumari, Christopher J. Panebianco, Joel D. Boerckel et autres

Osteoporotic bone degeneration involves progressive deterioration of trabecular microarchitecture, yet most scaffold-based bone tissue engineering studies evaluate osteogenesis in structurally favorable architectures that poorly represent compromised bone environments. Here, we establish a degeneration-inspired Voronoi scaffold platform in which point spacing serves as …

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Plasma-Enabled Multiscale Coupling of Architecture and Biointerfaces Drives Osteogenesis in 3D-Printed Gyroid Scaffolds

Pratheesh Kanakarajan Vijaya Kumari, J.F. Carpenter, Barnett Cleon, Christopher J. Panebianco et autres

Engineering functional bone scaffolds can be enhanced by integrating biologically instructive nanoscale surface features (e.g., nanotopography and nanoroughness), micro-scale geometric cues (e.g., curvature and porosity), and macro-scale mechanical properties (e.g., bulk stiffness); however, these length scales are often optimized independently. Here, we …

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A Single Instance of Joint Overloading Results: in Persistent Changes to the Synovial Cell Landscape

Kevin G. Burt, Vu Nguyen, Yasaman Moharrer, Joel D. Boerckel et autres

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Stress vesicles link epidermal mechanotransduction to stem cell differentiation

Sixia Huang, Paola Kuri, Jonathan Zou, Adriana Blanco et autres

The skin exhibits extraordinary plasticity, enabling it to adapt to mechanical changes in the environment. While transient deformations are accommodated without lasting structural effects, sustained mechanical stress induces durable tissue changes. To investigate if these responses are mediated by shifts in epidermal …

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CRISPR-mediated conditional mutagenesis of Smad1/5/8 reveals BMP/GDF signaling restricts postnatal bone overgrowth

Rimma Levina, Alexander J. Weitzel, Alexander Y. Liu, Benjamin E. Low et autres

Abstract The BMP/GDF branch of TGF-β signaling regulates diverse aspects of skeletal biology, from skeletal development to maintenance and repair. However, the complexity, redundancy, and pleiotropy of BMP/GDF signaling have hamstrung a genetic dissection of its activities in different cell types over …

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Maternal Exercise Rescues Fetal Akinesia‐Impaired Joint and Bone Development

Christopher J. Panebianco, Yuming Huang, Nidal Khatib, Devin C. Gottlieb et autres

ABSTRACT Fetal movements exert mechanical forces that shape the developing skeleton. Conditions that impair fetal movement can cause skeletal defects, but interventions are limited. Here, we show that maternal wheel running exercise regulates fetal skeletal development in mice. In wild‐type fetuses, maternal …

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Poroelastic mechanical loading disrupts cytoskeletal symmetry in 3D architected scaffolds

Kailin Chen, Mistica Lozano Perez, Erin E. Berlew, Tianbai Wang et autres

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Erythroid precursors regulate local oxygen tension and repair outcomes in the bone marrow niche

Annemarie Lang, Joseph M. Collins, Madhura P. Nijsure, Simin Belali et autres

Oxygen tension dynamically regulates stem cell fate and tissue regeneration, yet how local oxygen availability is controlled within the bone marrow niche remains poorly understood. While bone marrow injury, such as by bone fracture, disrupts marrow vasculature, the consequences for local oxygen …

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Mechanotransductive feedback control of endothelial cell motility and vascular morphogenesis

Devon E. Mason, Paula Camacho, Megan E. Goeckel, Brendan Tobin et autres

Vascular morphogenesis requires persistent endothelial cell motility that is responsive to diverse and dynamic mechanical stimuli. Here, we interrogated the mechanotransductive feedback dynamics that govern endothelial cell motility and vascular morphogenesis. We show that the transcriptional regulators, YAP and TAZ, are activated …

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