Nicolas Doisne

Cardiac toxicity of both chemical warfare nerve agent and organophosphate pesticide: Electrophysiological and non-cholinergic metabolic alterations in an in vitro model

Quentin Raffenne, Ilyana Siafi-Mili, Fabien Chantegreil, Léonie Briand et autres

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Gene Therapy with the N-terminal Fragment of Na v 1.5 for Cardiac Channelopathies: A Novel Transcomplementation Mechanism Potentiating the Cardiac Sodium Current

Marie Gizon, M Ferrand, Vincent Fontaine, Nathalie Mougenot et autres

ABSTRACT BACKGROUND Cardiac channelopathies, caused by mutations in ion-channel genes, can lead to sudden cardiac death (SCD) via ventricular arrhythmias. Brugada syndrome (BrS) is a rare inherited channelopathy characterized by a unique ECG pattern and a high incidence of ventricular fibrillation leading …

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Impacts of a high-fat diet on the metabolic profile and the phenotype of atrial myocardium in mice

Nadine Suffee, Elodie Baptista, Jérôme Piquereau, Maharajah Ponnaiah et autres

AIMS: Obesity, diabetes, and metabolic syndromes are risk factors of atrial fibrillation (AF). We tested the hypothesis that metabolic disorders have a direct impact on the atria favouring the formation of the substrate of AF. METHODS AND RESULTS: Untargeted metabolomic and lipidomic …

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In vivo Dominant-Negative Effect of an SCN5A Brugada Syndrome Variant

Nicolas Doisne, Marta Grauso, Nathalie Mougenot, Michel Clergue et autres

Loss-of-function mutations in the cardiac Na+ channel α-subunit Nav1.5, encoded by SCN5A, cause Brugada syndrome (BrS), a hereditary disease characterized by sudden cardiac death due to ventricular fibrillation. We previously evidenced in vitro the dominant-negative effect of the BrS Nav1.5-R104W variant, inducing …

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High Fat Diet Stimulates Beta-Oxidation, Alters Electrical Properties and Induces Adipogenicity of Atria in Obese Mice

Nadine Suffee, Elodie Baptista, Jérôme Piquereau, Maharajah Ponnaiah et autres

SUMMARY Metabolic disorders such as obesity are risk factors of atrial fibrillation, not only by sharing comorbidities but likely through their direct impact on atria, notably its adipogenicity. Here, we submitted mice that lack cardiac adipose tissue to a high fat diet …

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High fat diet induces change in mitochondria respiration, activation of K-ATP channel and action potential shortening in mice

Elodie Baptista, Nadine Suffee, Nicolas Doisne, Jérôme Piquereau et autres

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Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS

Marcin Bączyk, Najwa Ouali Alami, Nicolas Delestrée, Clémence Martinot et autres

Excessive excitation is hypothesized to cause motoneuron (MN) degeneration in amyotrophic lateral sclerosis (ALS), but actual proof of hyperexcitation in vivo is missing, and trials based on this concept have failed. We demonstrate, by in vivo single-MN electrophysiology, that, contrary to expectations, …

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Microtubule polymerization state and clathrin-dependent internalization regulate dynamics of cardiac potassium channel

Dario Melgari, Camille Barbier, Gilles Dilanian, Catherine Rücker‐Martin et autres

Ion channel trafficking powerfully influences cardiac electrical activity as it regulates the number of available channels at the plasma membrane. Studies have largely focused on identifying the molecular determinants of the trafficking of the atria-specific KV1.5 channel, the molecular basis of the …

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Distinct calcium/calmodulin-dependent serine protein kinase domains control cardiac sodium channel membrane expression and focal adhesion anchoring

Adeline Beuriot, Catherine A. Eichel, Gilles Dilanian, Florent Louault et autres

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Distinct CASK domains control cardiac sodium channel membrane expression and focal adhesion anchoring

Adeline Beuriot, Catherine A. Eichel, Gilles Dilanian, Florent Louault et autres

ABSTRACT Membrane-associated guanylate kinase (MAGUK) proteins function as adaptor proteins to mediate the recruitment and scaffolding of ion channels in the plasma membrane in various cell types. In the heart, the protein CASK (Calcium/CAlmodulin-dependent Serine protein Kinase) negatively regulates the main cardiac …

fr (code pays fourni par la source)

Microtubule polymerization state and clathrin-dependent internalization regulate dynamics of cardiac potassium channel

Dario Melgari, Camille Barbier, Gilles Dilanian, Catherine Rücker‐Martin et autres

ABSTRACT Ion channel trafficking powerfully influences cardiac electrical activity as it regulates the number of available channels at the plasma membrane. Studies have largely focused on identifying the molecular determinants of the trafficking of the atria-specific K V 1.5 channel, the molecular …

fr (code pays fourni par la source)

P3483Diet governs metabolic and electrical properties of the atrial myocardium in mice

Elodie Baptista, Nicolas Doisne, Jérôme Piquereau, Farid Ichou et autres

Abstract Background Obesity is associated with an increased risk of atrial fibrillation. This epidemiologic observation may not only be due to shared co-morbidities but also from direct impacts of metabolic disorders on myocardium Here, we examined the impact of high fat diet …

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